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Expression of Ldh-c (Sperm-Specific Lactate Dehydrogenase Gene) in Skeletal Muscle of Plateau Pika, Ochotona curzoniae, and its Effect on Anaerobic Glycolysis

Expression of Ldh-c (Sperm-Specific Lactate Dehydrogenase Gene) in Skeletal Muscle of Plateau Pika, Ochotona curzoniae, and its Effect on Anaerobic Glycolysis

Yang Wang1, Zhide Cheng2, Mengjie Tang3, Haixia Zhou1, Xiaolu Yuan4,Muhammad Aqeel Ashraf5, Shuting Mao1 and Jing Wang1,*

1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Zhengzhou University, Jianshe East 1 Road, Zhengzhou 450000, Henan, China.
2Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan, Hubei, China.
3Hunan Provincial Cancer Hospital, The Affiliated Tumor Hospital of Xiangya Medical School of Central South University, Changsha, Hunan, China.
4Deptt. Pathology and Pathophysiology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei, China
5Faculty of Science and Natural Resources, University Malaysia, Sabah 88400 Kota Kinabalu Sabah, Malaysia 

Yang Wang and Zhide Cheng contributed equally to this study.

*      Corresponding author: yangwangmd@163.com

Fig 1

The plateau pika and its inhabitation environment on Qinghai-Tibetan Plateau. A, Qinghai-Tibetan Plateau; B, Plateau pika on plateau.

Fig 2

Chemical and molecular structure of pyruvate and N-isopropyl oxamate.

Fig 4

Comparison of LDH, LD, ATP and their inhibition rates of plateau pika skeletal muscle with treatment. A, LDH activities in the skeletal muscle of plateau pika with treatment; B, LD contents in the skeletal muscle of plateau pika with treatment; C, ATP levels in the skeletal muscle of plateau pika with treatment; D, Inhibition rates of LDH, LD and ATP in the skeletal muscle with treatment. All data were expressed as mean±SD; sample size was 8 for each group. **p<0.01.

Fig 5

Amino sequence comparison of LDHA (A), LDHB (B) and LDHC (C) of amino homologous tree of Rattus norvegicus, Mus musculus, Bos taurus, Homo sapiens and Ochotona curzoniae. D, Amino homologous comparison of pika LDHA, LDHB and LDHC.

Fig 3

The expression level of Ldh-c in plateau pika skeletal muscle. A, The expression level of Ldh-c mRNA in plateau pika skeletal muscle. M: marker; B, The expression level of LDH-C protein in plateau pika skeletal muscle. Mean ± SD, n = 8.

Pakistan Journal of Zoology (Associated Journals)

April

Vol. 49, Iss. 2, Pages 425-759

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