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Evaluation of Different IgY Preparation Methods and Storage Stability as Potential Animal Feed Supplement

Evaluation of Different IgY Preparation Methods and Storage Stability as Potential Animal Feed Supplement

Shikun Ge1, 2, Yanli Yang3, Brindha Chelliappan4, 5, Antonysamy Michael5, Fagang Zhong1 and Xiaoying Zhang1, 2, 3, 4*

1State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang, 832000, China
2Centre of Molecular and Environmental Biology, University of Minho, Department of Biology, Campus de Gualtar, 4710-057 Braga, Portugal
3College of Veterinary Medicine, Xinjiang Agricultural University, Xinjiang, 830052, China
4College of Veterinary Medicine, Northwest A & F University, Post Box 19, Xinong Rd. 22, Yangling, Shaanxi Province 712100, China
5PSG College of Arts and Science, Bharathiar University, Coimbatore, Tamil Nadu, 641014, India

*      Corresponding author: zhang.xy@nwsuaf.edu.cn; zhang@bio.uminho.pt

ABSTRACT

Egg yolk can be used as feed additives to improve the immune status of animals and as passive therapy for veterinary diseases, and the egg yolk has proved to be a promising source of antibodies. However, the extraction methods and storage stability of IgY antibodies from egg yolk for feed supplements remain major problems in mass production. Based on Based on IgY antibody titer, IgY thermostability, IgY acid and alkali resistance, resistance to trypsin and pepsin, four different IgY purification methods (PEG extraction, water dilution method, the effect of liquid egg yolk and egg yolk powder preparations) on the activity of IgY antibodies was investigated. The results showed that IgY was stable below 70 oC, and the activity was significantly lost when it exceeded 70 °C; IgY was relatively stable in the range of pH 4-10, and the activity was significantly lost beyond this range; IgY had certain resistance to pepsin and trypsin. Among all four purification methods, the IgY antibody titer in liquid egg yolk and egg yolk powder was significantly higher than the other two methods. Moreover, egg yolk powder can prevent bacterial contamination and inactivation due to the formation of sediment. This study provide basic data for IgY application in animal feed supplements.
 

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Pakistan Journal of Zoology

December

Vol. 52, Iss. 6, Pages 2027-2426

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