Efficiency of entomopathogenic nematodes (Rhabditida) against Saccharococcus sacchari (Cockerell) (Homoptera: Pseudococcidae) under laboratory conditions
Efficiency of entomopathogenic nematodes (Rhabditida) against Saccharococcus sacchari (Cockerell) (Homoptera: Pseudococcidae) under laboratory conditions
Ahmed S.M.H. EL Roby
ABSTRACT
The objective of this study was to study the efficacy of four indigenous and one exotic isolated entomopathogenic
nematodes (EPNs) against sugarcane mealybug Saccharococcus sacchari under laboratory conditions (27 ± 2 °C,
RH 65 ± 10%). The EPNs used in the study are Steinernema sp. (strain: AT4), Steinernema sp. (strain: EMB),
Heterorhabditis bacteriophora (strain: EKB20), Heterorhabditis sp. (strain: EIK) and Steinernema glasri (strain:
New Jersey). The bioassays were carried out on Petri dishes containing parts of pumpkin infested with adult females
of S. sacchari, and were sprayed with EPN juveniles. Results showed that H. bacteriophora strain (EKB20) and
Heterorhabditis sp (EIK) were more efficient with higher pathogenicity and virulence in the laboratory than the
other strains and gave the highest corrected mortality percentage in the infestation (from 68.59% to 70.83%). The
LC50 were 25.42 and 45.5 IJs/ insect for the two strains, respectively.
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June 2018
Pakistan Journal of Nematology, Vol. 36, Iss. 1, Pages 1-115
