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Effect of hcp1 of the Type VI Secretion System on Biological Characteristics of F18 Shiga Toxin-Producing Escherichia coli

Effect of hcp1 of the Type VI Secretion System on Biological Characteristics of F18 Shiga Toxin-Producing Escherichia coli

Yang Yang1,2, Hong Zhou3, Ji Shao1,2, Xinyi Zhang1,2, Pengpeng Xia1,2, Mingxu Zhou4,5, Qiangde Duan1,2 and Guoqiang Zhu1,2*

1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
2Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
3School of Basic Medical Science, Southwest Medical University, Luzhou 646000, China
4Institute of Veterinary Immunology and Engineering, National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
5Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China
 
Yang Yang and Hong Zhou contributed equally to this work.
 
* Corresponding author: yzgqzhu@yzu.edu.cn

Fig. 1.
Results of growth curve (A) and motility assay (B).
Notes: A, there was no significant difference in growth rate among the three strains. B, there was no significant difference in biofilm formation ability among the three strains.
Fig. 2.

The motility, adherence and colonization assays of bacterial. A, Flagella related motility assays. A1: F18ab; A2: F18abΔhcp1; A3: F18abΔhcp1/phcp1. B, Adherence ability towards IPEC-J2 cell line. C, Invasion ability towards IPEC-J2 cell line. D, Intestinal colonization test.  * Significant difference, p<0.05.

Fig. 3.
Vero cell cytotoxicity assay.
* Significant difference, p<0.05.
Fig. 4.
Transcriptional levels of virulence related genes.
*: The gene transcriptional levels compared with F18ab, P<0.05

Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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