Effect of 25(OH)D3 Supplementation in Sows’ Diets on Heart Development in Neonatal Piglets
Qingyue Han1, Ying Li1, Jichang Deng1, Kunxuan Huang1, Yanyang Yang1, Quanwei Li1, Zhuowei Zhang1, Na Qiao1, Yanju Ji2, Khalid Mehmood3, Sarfaraz Ali Fazlani4, Hui Zhang1 and Zhaoxin Tang1*
Performance and cardiac index analysis of different treatment groups. Daily weight gains of different groups piglets in day 7, 14, 21 (A); Average daily weight gain (B); Effects of 25(OH)D3 and Ca2+ supplementation in sows’ diets on cardiac index (C).
Effect of Vit D3 on histological structure of heart piglets. Note development of myocardial fiber A, control; B, low calcium treatment; C, Vit D3 treatment; D, Vit D3 + low calcium treatment. Stain: Hematoxilyn & Eosin.
Quantitative RT-PCR analysis of cardiac insulin-like growth factor (IGF). The mRNA expressions levels of IGF, IGF1R, IGF2 and IGFBP3 genes in the control, low calcium group (LCa), 25(OH)D3 group (VD) and low calcium + 25(OH)D3 group (LCa+VD) groups in the cardiac from piglets as determined by qRT-PCR. The data represent the mean ± SD.
Effect of 25(OH) D3 and Ca2+ on PCNA and ki67 expression in neonatal piglets. *P< 0.05; expression levels were normalized to the levels of the geometric mean of GAPDH gene expression (mean ± SD).