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Determination of Polymorphisms in the GDF5 and EPS8 Genes by HRM Analysis in Holstein Cattle

Determination of Polymorphisms in the GDF5 and EPS8 Genes by HRM Analysis in Holstein Cattle

Zeynep Sonmez1 and Memis Ozdemir2,*

1Department of Animal Science, Faculty of Agriculture, Igdir University, 76000 Igdir, Turkey
2Department of Animal Science, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey

*      Corresponding author: ozdemirm@atauni.edu.tr

ABSTRACT

The aim of this study was to determine new polymorphisms in GDF5 (growth differentiation factor 5 (CDMP-1)) and EPS8 (epidermal growth factor receptor substrate 8). After the PCR analysis of the identified gene regions of 72 head Holstein cattle, samples were separated by the high-resolution melting analysis (HRMA), and sequence analysis was applied to some randomly selected samples. New polymorphisms were identified in the determined genes and gene regions as a result of the sequence analysis. In the 1st exon of the EPS8 gene, 9 polymorphic regions were identified as g.94554132C>T, g.94554252G>C, g.94554348T>G, g.94554354C>G, g.94554372C>G, g.94554389A>G, 94554392C>G, 94554399A>G, and 94554439C>G. Within the region examined in the 2nd exon of the EPS8 gene, only the g.94555920T>G polymorphism was identified. In the 1st exon of the GDF5 gene, the g.65340723G>A and g.65340727T>C polymorphisms were identified. In the 2nd exon of the GDF5 gene, the g.65340902A>G polymorphism was identified. No polymorphism was found in the examined 2nd intron region of the GDF5 gene. As a result, new polymorphic regions were detected on EPS8 and GDF5 genes by HRMA, genotype and allele gene frequencies of polymorphic regions were determined. Hardy-Weinberg equilibrium test generally shown that the distribution of genotype frequencies in polymorphic regions is in genetic equilibrium.
 

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Pakistan Journal of Zoology

October

Vol. 53, Iss. 5, Pages 1603-2000

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