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Acute Effect of Epidermal Growth Factor on Phosphate Diffusion across Intestinal Mucosa of Hens using the Ussing Chamber System

Acute Effect of Epidermal Growth Factor on Phosphate Diffusion across Intestinal Mucosa of Hens using the Ussing Chamber System

Xiaopeng Tang1,2, Rejun Fang3,4,*, Guochang Pan1,2 and Kangning Xiong1,2,*

1School of Karst Science, Guizhou Normal University, Guiyang, China
2State Key Laboratory Cultivation for Karst Mountain Ecology Environment of Guizhou Province, Guiyang, China
3College of Animal Science and Technology, Hunan Agricultural University, Changsha, China
4Hunan Co-Innovation Center of Animal Production Safety, Changsha, China

*      Corresponding author: fangrj63@126.com; xiongkn@163.com

 

Fig. 1.

The structure of Ussing chamber system. A, the panorama of the Ussing chamber; B, electronic circuitry (VCC-MC6); C, diffusion chamber (6 channels); D, diffusion pool and receiving room; E, inserts (P2313); F, electrodes.

Fig. 2.

The LDH activity of isolated small intestine. A, LDH activity of duodenum; B, LDH activity of jejunum; C, LDH activity of ileum. Data are represent as mean ± SD, n=4. a, b and c, means within error bars with different letters differ significantly (P < 0.05).

Fig. 3.

Effect of epidermal growth factor on transepithelial resistance (TEER) of isolated small intestine. A, duodenum; B, jejunum; C, ileum. Data are represent as mean ± SD, n=4. * means P < 0.05.

Fig. 4.

Effect of epidermal growth factor on Pi absorption rate of isolated small intestine. A, Pi absorption rate of duodenum; B, Pi absorption rate of jejunum; C, Pi absorption rate of ileum. Data are represent as mean ± SD, n=4. * means P < 0.05.

Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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