Research Article

 

Quercetin Mitigates Vanadium-Pentoxide-Induced Oxidative Stress and Reproductive-Hormone Disruption in Male Rats

 

Tamer S Imam1, Walaa M. Elhady1, Rasha Thabet Metwaly Alam*2, Haiam A Mohammed3

1Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Sharkia, Egypt; 2Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Sharkia, Egypt; 3Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Sharkia, Egypt.

 

Received | June 25, 2019; Accepted | August 19, 2019; Published | April 15, 2020

*Correspondence | Rasha Thabet Metwaly Alam, Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Sharkia, Egypt; Email: [email protected]

Citation | Imam TS, M Elhady W, Alam RTM, Mohammed HA (2020). Quercetin mitigates vanadium-pentoxide-induced oxidative stress and reproductive-hormone disruption in male rats. Adv. Anim. Vet. Sci. 8(5): 490-498.

DOI | http://dx.doi.org/10.17582/journal.aavs/2020/8.5.490.498

ISSN (Online) | 2307-8316; ISSN (Print) | 2309-3331

Copyright © 2020 Alam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 

INTRODUCTION

 

Vanadium pentoxide (VP; V2O5) is coming from an industrial perspective, the most important stable salt of vanadium, it is used as an oxidizing agent, a catalyst, and vanadium alloys production (Budavari et al., 1996; Rivedal et al., 1990; Rojas et al., 1996). Vanadium-containing compounds derived from combustion of fuel products contribute to air pollution. Vanadium compounds also have a number of medicinal uses, including a vaginal contraceptive (D’ Cruz et al., 1998), antitumor (Olopade et al., 2009), and anti-diabetic (Hu et al., 2010) agents. Moreover, vanadium compounds have also been shown to produce neurotoxicity in rats (Garcia et al., 2005), enhance the formation of micronucleus (Zhang et al., 1993), and cause DNA damage in human leukocytes (Rojas et al., 1996). However, VP impairs the function of the cardiovascular system, increases respiratory disorders, and induces alterations in immune responses in mice (Pinon-Zarate et al., 2008). Additionally, VP leads to necrosis of testicular tissues, destruction of seminiferous tubules, and reduction in spermatogonia in guinea pigs (Uche et al., 2008).

 

Quercetin, a flavonoid which is present in many vegetables and fruits, it has properties that may reduce the risk of infection (Davis et al., 2009), lipid peroxidation and inflammation (Aguirre et al., 2011), as well as helping to protect cells from injury, and to increase antioxidant capacity (Camargo et al., 2011). The antioxidant properties of quercetin make it a hepato-protective agent (Vidhya and Indira, 2009), and it has immunostimulatory and anti-inflammatory properties, including enhancement of immunoglobulin production and reduction of the certain cytokines release (Alam et al., 2016; Li et al., 2016).

 

This work was aimed to explore, the protective effects of quercetin against VP toxicity with respect to oxidative stress, sperm parameters, immunohistochemical expression of actin and the histopathology of testicular tissue.

 

MATERIALS AND METHODS

 

Chemicals

Analytical grade Vanadium oxide (V2O5, 223794, orange powder), was purchased from Sigma (USA, St Saint Louis, MO). Quercetin was prepared daily (dissolved in saline) and purchased from Sigma.

 

Experimental Animals

Healthy male adult Wistar albino rats (6 weeks old, n = 40, 150–200 g BW), were obtained from laboratory house of animal (Faculty of Veterinary Medicine, Zagazig University). Animals were kept in cages from metal, at 45–55% relative humidity and 25°C, with a light–dark cycle (12 h). Basal diet of rodent and water were supplied ad libitum throughout the experiment. Rats underwent a 2-week adaptation period prior to experimentation. Management and housing of the animals, also the experimental design were approved by the ethics committee of research (Faculty of Veterinary Medicine, Zagazig University, Egypt) and performed as stipulated in the Guide for Use and Care of Animals Laboratory Guidelines of the National Health Institutes. Efforts were done to reduce suffering of the animal.

 

Rats were equally classified into four groups, then given the indicated treatment agents by gavage daily, five times each week, for 65 days. Control group administered distilled water (0.5 ml) per treatment. The quercetin group received quercetin at 50 mg/kg BW (Ding et al., 2012). The VP group received VP which dissolved in water (distilled) at a dose of 2.5 mg/kg (one-quarter of the previously determined oral LD50 of 10 mg/kg [Lewis, 2000]). The quercetin+VP group received quercetin followed by VP as the group II/III doses.

 

After 65 days, under light ether anesthesia, rats were euthanized (cervical dislocation). Blood samples (2 ml) were collected from each rat into gelatin-containing vacuum test tubes without anticoagulant for serum separation. The testes were dissected from each rat for further investigation of antioxidant and oxidative-stress markers and histopathology.

 

Biochemical Analysis

Samples of blood were collected from rats, left for 30 min (room temperature) to enable clotting, and were centrifuged at 322 × g for 20 min for serum separation, which was stored (−20°C) until further use. Total testosterone levels were determined with ELISA kit of rat (China, Cusabio; Wuhan). Serum luteinizing hormone levels was assessed with a rat ELISA kit (USA, MyBioSource; San Diego, CA), and the levels of lactate dehydrogenase were measured according to Buhl and Jackson (1978). Levels of acid phosphatase (ACP) were measured with kits obtained from Quimica Clinica Aplicada Company (Spain, Amposta) according to a previously described method (Moss, 1984). The assay of ACP depends on the p-nitrophenyl-phosphate hydrolysis in acidic medium, with spectrophotometric quantification of liberated p-nitrophenol by absorbance at 420 nm.

 

Sperm Analysis

Sperm motility percent: Cauda epididymis was removed from the testis from each rat, placed in 2 ml normal saline at a sterile Petri dish (37°C), and created a small opening for facilitating the sperm passage to obtain the epididymal suspension (Eleiwa et al., 2018). A drop of the prepared suspension was examined under a light microscope for evaluation of spermatozoon motility; 200 spermatozoa were analzed in five microscopic fields, for assessment the percentage of motility (Slott et al., 1991).

 

Sperm-cell concentration: The semen suspension was diluted 1:4 in formol saline (containing a little drops of formalin 40%, for killing the spermatozoa); then the prepared suspension was well mixed and used to count the sperm (Robb et al., 1978).

 

Sperm abnormalities: The eosin–nigrosin stain (two drops) were mixed with each formalin-treated semen suspension on a microscope slide, and a good film was made, dried in the air, and examined. Each sample, 200 spermatozoa were assessed randomly in different fields for the abnormalities (head and/or tail), and the total abnormality percentage was recorded (Filler, 1993).

 

Antioxidant enzymes and oxidative-stress markers: One testis was preserved in liquid nitrogen from each rat for enzymatic assessment. Testicular tissue (1 g) was homogenized with ice-cold phosphate buffer (10 ml, 7.4 pH) by an electrical homogenizer, and centrifuged for 15 min at 322 × g (4°C). The supernatant was collected and preserved (−80°C) until use. Levels of testicular reduced glutathione (GSH) were estimated according to Beutler et al. (1963), catalase was determined according to Aebi (1984), and the concentration of malondialdehyde was assessed according to Mihara and Uchiyama (1978).

 

Immunohistochemistry: Tissue sections (5 µm) from the testis were placed on slides coated with poly-L-lysine, then dewaxed (56°C for 30 min) and placed 5 min in xylene. All sections were hydrated in 96% ethanol and water (distilled). The expression of actin, smooth muscle Ab-1 (α-SMA) was determined by staining with a mouse monoclonal antibody (Catalog # MS-113-P0, Thermo Fisher Scientific, Fremont, CA, USA) according to Skalli et al. (1986). After binding of the secondary antibody, positive immunostaining was indicated by the formation of a brown precipitate at the site of binding under light microscopy.

 

Histopathological Examination

Samples from the testicular tissue were fixed in formalin, then dehydrated in ethanol (70%), then cleared in xylene, and finally embedded in paraffin. Sections (5 µm thick) were cut, mounted on slides, then rehydrated, and stained with H&E (hematoxylin and eosin) and examined microscopically (Suvarna et al., 2013).

 

Statistical Analysis

The data were represented by means ± SE for each group, and variations among different groups were analyzed by one-way ANOVA analysis of variance followed by Duncan’s multiple ranges post hoc test. A significant differences were considered at P <0.05.

 

RESULTS

 

Effects Of Treatment With Quercetin And Vp On Serum Biochemical Parameters

Compared with control group, serum total testosterone levels were not significantly different in rats administered quercetin, but were lower (P <0.05) in rats administrated VP (Table 1). The serum total testosterone levels were significantly (P <0.05) increased in the quercetin+VP group when compared with VP intoxicated group, although levels in the combined group were still lower than in the controls. Compared with control rats, serum LH was not vary significantly in the quercetin-administered group, but it was lower (P <0.05) in VP-treated rats. LH levels in rats co-treated with quercetin and VP were higher (P <0.05) than VP group. Serum LDH levels were reduced (P <0.05) in quercetin treated rats and significantly increased in the VP intoxicated rats when compared to control. LDH levels were significantly lower in the co-treated group than VP treated group (Table 1).

 

Effects of Treatment with Quercetin and Vp on Sperm Parameters

In relation to sperm quality, compared with the control rats, there was a non-significant change in sperm motility in the quercetin-treated group, but motility was lower (P <0.05) in the VP-treated rats. Motility in the combined (quercetin+VP) group was significantly (P <0.05) increased than the VP group, and showed a non-significant change in levels in the control (Table 2). Compared to control, the sperm count was significantly (P <0.05) increased in quercetin-treated rats and significantly (P <0.05) lower in VP-treated rats. The count was significantly (P <0.05) increased in the quercetin+VP co-treated rats than in VP-treated group. Compared with control, the rate of sperm abnormality was significantly higher in the VP group, whereas the rate was lower (P <0.05) in the co-treated group than VP-treated group (Figure 1).

 

Table 1: Effect of quercetin and vanadium pentoxide (VP) treatment on serum biochemical parameters in rats.

 

Parameter	Group
	Control	Quercetin	VP	Quercetin +VP
Total testosterone (ng/dl)	a352.76 ± 3.28	a357.23 ± 0.91	c188.47± 0.75	b230.34 ± 1.48
LH (mIU/ml)	a1.30 ± 0.032	a1.26 ± 0.034	c0.16 ± 0.004	b0.62 ± 0.005
LDH (U/l)	c152.40 ± 3.48	d129.80 ± 0.37	a1546.2 ± 2.73	b874.00 ± 13.84

Values shown are means ± SE (n = 5). Means within the same row labelled with different superscripts are significantly different (p ≤0.05).

Control treatment was 0.5 ml/kg distilled water; quercetin was 50 mg/kg quercetin; VP was 2.5 mg/kg VP; and quercetin+VP was 50 mg/kg quercetin + 2.5 mg/kg VP.

 

Table 2: Effects of quercetin and vanadium pentoxide (VP) treatment on sperm motility %, count and abnormality % in male albino rats.

 

Parameter	Group
	Control	Quercetin	VP	Quercetin +VP
Sperm motility %	a85.00 ± 1.58	a84.00 ± 1.87	b69.00 ± 1.00	a84.00 ± 1.00
Sperm count (mL× 125×104)	b73.20 ± 0.96	a85.00 ± 1.09	d40.60 ± 0.51	c68.00 ± 1.22
Sperm abnormalities %	c9.60 ± 0.20	c10.01 ± 0.26	a23.86 ± 0.84	b16.96 ± 0.41

 

Values shown are means ± SE (n = 5). Means within the same row labelled with different superscripts are significantly different (p ≤0.05).

Control treatment was 0.5 ml/kg distilled water; quercetin was 50 mg/kg quercetin; VP was 2.5 mg/kg VP; and quercetin+VP was 50 mg/kg quercetin + 2.5 mg/kg VP.

 

Effects of Treatment with Quercetin and Vp on Antioxidant/Oxidative Stress Markers in Testicular Tissue

Quercetin administration did not significantly affect testi-

 

 

Table 3: Effect of quercetin and vanadium pentoxide (VP) treatment on antioxidant/oxidative-stress markers of testicular tissue in rats.

 

Parameter	Group
	Control	Quercetin	VP	Quercetin +VP
Acid phosphatase (U/g)	c85.98 ± 0.98	c86.01 ± 0.75	a133.07 ± 0.59	b111.56 ± 0.23
Glutathione (mmol/g)	a9.87 ± 0.021	a10.10 ± 0.038	c4.04 ± 0.08	b6.96 ± 0.27
Catalase (U/g)	b23.62 ± 0.53	a27.64 ± 0.74	d16.87 ± 0.28	c21.72 ± 0.34
Malondialdehyde (nmol/g)	c3.8 ± 0.09	d1.5 ± 0.13	a21.45 ± 0.33	b6.06 ± 0.09

 

Values shown are means ± SE (n = 5). Means within the same row labelled with different superscripts are significantly different (p ≤0.05).

Control treatment was 0.5 ml/kg distilled water; quercetin was 50 mg/kg quercetin; VP was 2.5 mg/kg VP; and quercetin+VP was 50 mg/kg quercetin + 2.5 mg/kg VP (2.5 mg/kg).

 

cular ACP, whereas VP toxicity provoked a significant (P <0.05) elevation in ACP compared with control group, and the ACP level in the quercetin+VP group was significantly lower (P <0.05) than VP group (but still higher than control group). The reduced glutathione concentration was lower (P <0.05) in the VP-intoxicated group when compared with control, and significantly (P <0.05) increased in quercetin+VP co-treated rats than VP-treated rats (but still lower than in the control group). Testicular catalase activity was significantly (P <0.05) increased in quercetin-treated group and decreased in VP-treated group than in controls, and also higher in quercetin+VP co-treated rats than in VP-treated rats (but still lower than in controls). Lipid peroxidation (malondialdehyde) in testicular tissue was lower in quercetin-treated rats and increased (P <0.05) in VP-treated rats than in controls, and significantly (P <0.05) lower in the co-treated group compared to VP-treated group (but still higher than in controls). Table (3).

 

Effects of Quercetin and Vp Treatment on Actin Immunostaining in Testicular Tissue

Immunohistochemical examination of testicular tissue revealed alterations in the myoid cells through α-SMA immunostaining. The control and quercetin-treated groups showed strong α-SMA immunostaining which represented by brown expression, whereas VP-treated rats showed weak α-SMA immunostaining, and the quercetin+VP co-treated rats showed moderate α-SMA immunostaining (Figure 2).

 

 

Effects of Treatment with Quercetin and Vp on Testicular Histopathology

Histopathological evaluation of testicular tissues revealed normal histological architectures of closely packed seminiferous tubules lined with seminiferous epithelial cells and interstitial tissue containing Leydig cells in the control and quercetin-treated group (Figure 3). In the VP-treated group, severe germinal epithelial atrophy was observed, and interstitial tissue was replaced with homogenous eosinophilic material and vacuoles. In the quercetin+VP co-treatment group, less histopathological disruption occurred than in the VP-treated group, and a sufficient number of spermatocytes and mild vacuolation of some spermatogonial cells was observed.

 

 

DISCUSSION

 

In the present work, rats intoxicated with VP had significant reduction in the serum total testosterone levels than control rats. This may be attributed to VP accumulation in testicular tissues, leading to disruption of cell function, a decrease in the synthesis of testosterone, or inhibition of androgenic enzyme activity in the testes. VP has previously been shown to reduce the growth of accessory sex organs and to decrease androgenesis in rats (Dehghani et al., 2002), that may be related to a reduction in the levels of serum LH. Results from previous studies have also recorded the toxic effects of vanadium in reducing the serum testosterone levels (Chandra et al., 2010; Vijaya Bharathi et al., 2015). These results have now demonstrated that co-treatment of quercetin can, to some extent, counteract the effects of VP treatment on testosterone levels; it is able to increase the levels of serum testosterone in rats (Ma et al., 2004).

 

LH, which is required for normal spermatogenesis in puberty (Russell et al., 1987), also, is the main regulator of the activities of androgenic enzyme in the testis (Shaw et al., 1979), and is responsible for maintaining normal testosterone levels (Ellis et al., 1982). We found that oral VP administration significantly reduced serum LH levels relative to those in controls, which may be attributed to stimulation of adrenocortical hormones that consequently suppresses LH secretion from the pituitary gland (D’Agostino et al., 1990). Although quercetin alone did not affect LH levels, simultaneous administration of quercetin and VP elevated serum LH relative to VP alone, possibly via direct stimulation of other sex organs to increase the secretion of LH, or by reduction of the accumulation of VP in tissues, thereby counteracting the effect of VP on the pituitary gland, and maintaining the level of LH.

 

LDH is an intracellular enzyme that is another index for VP toxicity. Our results have shown that serum activity of LDH is elevated in rats intoxicated with VP, which may be attributed to the interaction of accumulated vanadium with LDH and a coenzyme system (Kanthasamy et al., 1988; Shrivastava et al., 2007). Additionally, the activity of intracellular LDH significantly increased by VP treatment (Song et al., 1998), and this activity has been shown to be associated with damage in Sertoli and germ cells (Pant and Srivastava, 2003), and LDH rapid release to the circulation after membrane damage. Our results showed that quercetin alone reduced LDH activity, and that administration of quercetin with VP reduced LDH activity compared with VP alone, suggesting the prevention of testicular injury, possibly by reduction of LDH release from cells (Zou et al., 2010; Kumar et al., 2016) through minimizing the oxidative stress and maintaining the cellular integrity.

 

VP toxicity leads to clinical problems such as impairment of spermatogenic activity and reduction of male fertility. Our results indicated that VP intoxication affects spermatogenesis, resulting in a decrease in the sperm motility percentage and count, with an elevation the sperm abnormality percentage, which may be attributed to accumulation of VP and/or its metabolites in the testes. This accumulation of VP has been shown to cause reductions in expression of gamma-tubulin in testicular tissue, which may affect the spermatogenic process and cell division in the testes (Mussali-Galante et al., 2005). Chronic vanadium toxicity might also interfere with the production of testosterone, thereby decreasing sperm count and motility in association with excessive reactive oxygen species and lipid peroxidation production in the testes (Jain et al., 2007). Moreover, vanadium toxicity may increase levels of sperm-cell abnormalities (Cooper, 2007; Uche et al., 2008; Vijaya Bharathi et al., 2015). By contrast, quercetin has an androgenic effect, and it can ameliorate the toxic effect of VP in the co-treated rats by improving sperm quality (concentration, viability, and motility), which may be attributed to stimulation of epidydimal and testicular function (Taepongsorat et al., 2008) and stimulation of the secretion of testosterone from the hypothalamic–pituitary–gonadal axis (Ma et al., 2004).

ACP, a testicular enzyme that is located in the lysosomes of Leydig cells, is a sensitive marker of VP toxic effects on spermatogenesis in the male reproductive system. We observed elevated testicular ACP activity in VP-treated rats, indicating degeneration of the testes, which may be a consequence of a low level of testosterone. Vanadium toxicity has previously been shown to increase the activity of ACP measured in guinea pig testes (Uche et al., 2008). Elevation of ACP by VP was mitigated by quercetin, which may be due to protection of testicular tissue from degeneration by toxic agents.

 

We found that VP toxicity significantly reduced testicular glutathione and catalase levels, and elevated malondialdehyde levels, possibly through accumulation of VP and/or its metabolites in the testes, leading to damage of cellular enzymes (Donaldson et al., 1985) and an increase in the release of inflammatory cytokines and chemokine from cells (Cooper, 2007), that exhaust antioxidant enzymes to overcome the oxidative stress, resulting in injury and degeneration of testicular tissue. Our results suggest that elevation of malondialdehyde levels may be related to the failure of cellular antioxidant defenses, such as glutathione and catalase. Similar findings have been made previously by Chandra et al. (2007) and Vijaya Bharathi et al. (2015). We found that quercetin provided some protection from these effects of VP, with co-treatment leading to increased cellular glutathione and catalase levels and decreased malondialdehyde in testicular tissues, compared with VP alone. Quercetin has previously been identified as a highly effective antioxidant agent (Azab et al., 2013), and it may act as a free-radical scavenging and ion-chelating agent (Joshi et al., 2011), thereby elevating cellular antioxidant-enzyme levels. Furthermore, quercetin is able to reduce lipid peroxidation of the cell membrane and inflammatory reactions in injured tissues (Aguirre et al., 2011; Alam et al., 2016; Li et al., 2016). Antioxidant nature of quercetin also explains its ability to mitigate testicular damage induced by VP, which is attributed to free radical reactions which induce oxidative stress that cause DNA damage and breakage (Sakurai et al., 1995; Shi et al., 1996; Aherne and O,Brien, 2000).

 

Actin is one of the main cytoskeletal proteins which regulate the process of spermatogenesis in the testes. This protein is involved in cell division, organization, and morphology, as well as the integrity of hematotesticular barrier. We found that VP, which produces changes in the normal function of the testes that lead to infertility, decreased myoid-cell actin immunostaining in the testicular tissue. Similar results have previously been obtained in mice (Rodríguez-Lara et al., 2016). Vanadium compounds induce alterations in actin, by rotein tyrosine phosphatases inhibition (Bennett et al., 1993; Ramos et al., 2011), and can also affect actin polymerization. Our results agree with those of Rodrı´guez-Lara et al. (2016). Damage to the actin cytoskeleton could explain the toxic effects of VP associated with impairment of fertility. Concurrent treatment with quercetin might have reduced the damaging effect of VP on actin by virtue of the antioxidant effects of quercetin, and might also have reduced the accumulation of VP in testicular tissue, protecting the cell membrane from oxidative stress.

 

All of our results indicating the testicular toxicity of VP were corroborated by the histopathology of the testes, which showed cystic dilatation of the seminiferous tubules, severe interstitial edema, spermatogenesis arrest with few spermatocytes and vacuolation of the spermatogonial cells and Leydig cells. Similarly, the protective effect of quercetin in relation to VP toxicity was evident in the reduction of histopathological disruption in the presence of quercetin.

 

Taken together, our study indicates that VP induces disturbances in male androgenic activities and testicular functions in rats, but that concurrent treatment with quercetin can reduce the induction of oxidative damage by VP and maintain normal steroidogenesis. Our results suggest the feasibility of using this natural antioxidant to mitigate the VP deleterious effects in male reproductive organs.

 

Acknowledgments

 

The authors wish to thank Dr. Nesma Ebrahim, lecturer of Histology, Zagazig University for technical assistance in processing the tissue for immunohistochemistry and histopathological examination.

 

Conflicts of Interest

 

The authors declare no conflicts of interest.

 

Author Contributions

 

Imam T. S. and W. M. Elhady performed the majority of the experiments and contributed to the data analysis and R.T.M. Alam wrote the manuscript, designed, supervised the study and checked the final manuscript.

 

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