Advances in Animal and Veterinary Sciences
Microsatellite Polymorphisms and its Relationship with Calving Interval and Gestation Period in Bali Cattle
I Ketut Puja1*, I Nyoman Sulabda2, I Nengah Wandia3
1Veterinary Genetics and Reproduction Technology Laboratory, Faculty of Veterinary Medicine, Udayana University; 2Veterinary Physiology Laboratory, Faculty of Veterinary Medicine, Udayana University; 3Veterinary Anatomy Laboratory, Faculty of Veterinary Medicine, Udayana University, Indonesia.Abstract | The present study was conducted to detect polymorphisms of microsatellites and a possible relationship with calving interval and gestation period in bali cattle. The genotypes for ILSTS026, BMS1987, BMS2646, INRA072, and BMS1248 loci that involved in reproduction traits were asses by polymerase chain reaction in bali cattle. Blood samples were obtained from 42 bali cattle. The result describedthat all markers were amplified successfully in bali cattle microsatellite loci. For the five microsatellites loci analyzed were detectedto be polymorphic. The number of 29 alleles were foundin all samples, with the variation of alleles varied from 3 to 8. The fragment size was 105 bp to 300 bp. The PIC varied between 0.5025 to 0.859. For this marker, the Expected and Observed heterozygosity in this study diversebetween 0.5981 (locus BMS2646) to 0.9048 (BMS1987 and INRA072) and 0.5981 (BMS2646 to 0.8943 (BM1248), respectively. The microsatellite loci INRA072 had a positive association with gestation period while BM2646 had a positive association with calving interval.These data provide evidence that bali cattle breed have good genetic variability, which prospects for future selecting programs, especially marker assistant selection.
Keywords | Bali cattle, Genotype, PIC, Polymorphisms, Reproduction trait
