Advances in Animal and Veterinary Sciences

Research Article
Adv. Anim. Vet. Sci. 3 (3S): 1 - 8. Special Issue-3 (Poultry diseases and the strategies for their control and prevention)

Immunomodulatory and Therapeutic Prospective of a Protein Supplement with Vitamins and Selenium (Multimune) against Chicken Infectious Anaemia in Broiler Chicks

Gopal Krishan1, Santosh Kumar Shukla2, Prakash Bhatt3, Rajesh Kumar4*, Ruchi Tiwari5, Yashpal Singh Malik6, Kuldeep Dhama7

1Department of Animal Husbandry, Himachal Pradesh, India; 2Department of Veterinary Medicine; 3Veterinary Clinics; 4Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Udham Singh Nagar, Uttarakhand - 263 145, India; 5Department of Veterinary Microbiology, College of Veterinary Sciences, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishwa Vidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh – 281001, India; 6Division of Biological Standardization; 7Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute (IVRI), Izatnagar, Uttar Pradesh – 243 122, India.

*Correspondence | Rajesh Kumar, Govind Ballabh Pant University of Agriculture and Technology, Uttarakhand, India; Email:

Chicken infectious anaemia virus (CIAV) is a well-established cause of generalized lymphoid atrophy accumulating severe immunosuppression subsequent to infection. In this study, we assessed the role of a protein supplement with vitamins and selenium (Multimune) against the CIAV infection in broiler chicks. Experiment was performed on 60 day-old broiler chicks grouped into 3 groups of 20 birds in each and all the birds were vaccinated against Newcastle Disease (ND) and infectious bursal disease (IBD). The chicks of group I served as healthy control and other 2 group chicks (group II and III) were exposed to CIAV (104.5 TCID50/0.1 ml). The Multimune was fed for 3 weeks to chicks of group III @1 gm / 10 birds. Successively, all the birds were examined for ND antibody titres, various haematological and biochemical parameters, organ: body weight ratios, and mean live body weight at weekly interval until 35th day of the study. The chicks of group II showed significant decline in the count of erythroid and myeloid cells, mean live body weight and organ: body weight ratios of lymphoid organs, and ND antibody titres, whereas upturn was observed in enzyme activities and uric acid values. In contrast, the immunosuppression was less severe in (group III in comparison to group II chicks. The haematological and biochemical parameters, mean live body weight and organ body weight ratios were significantly (P<0.05) higher in Multimune supplemented chicks. Present study highlights the use of Multimune as an effective immunomodulating agent in immune suppressed CIAV affected birds with reduced virus pathogenicity, enhanced immune responses and safeguards the virus prompted ill effects on bird’s growth performances.

Keywords | Chicken infectious anemia virus, Immunosuppression, Immunomodulator, Multimune, Chicken

Pakistan Journal of Zoology


Vol. 55, Iss. 4, Pages 1501-2000


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