Search for Rickettsia

Preliminary Development of an Enzyme-Linked Fluorescent DNA Aptamer-Magnetic Bead Sandwich Assay for Sensitive Detection of Rickettsia Cells

John G. Bruno¹*, Chien-Chung Chao^2,3, Zhiwen Zhang³, Wei-Mei Ching^2,3, Taylor Phillips¹, Allison Edge¹, Jeffery C. Sivils¹

Feasibility of Anti-Idiotypic Aptamers to Detect Rickettsia Exposure

John G. Bruno

...ere capable of emulating rickettsial antigens to enable sensitive detection of rickettsia exposure in humans who have seroconverted. Such anti-idiotypic aptamer antigens might also serve as rickettsial vaccines, if fully developed. The top five highest affinity R. rickettsii and top five R. typhi anti-Fab aptamers as assessed by enzyme-linked aptamer sorbent assay (ELASA) screening were sh...

Prevalence of Anaplasma marginale in Tropical Area of Khyber Pakhtunkhwa, Pakistan

Saira Gul^1,2, Sohail Ahmed²*, Tahir Usman¹*, Khalid Khan³, Sultan Ayaz¹, Saleha Gul⁴and Nawab Ali⁵

...se caused by a rickettsial parasite Anaplasma marginale. This study investigated the prevalence of A. marginale in cattle on different commercial farms in subtropical conditions of Khyber Pakhtunkhwa (KP). A total of 120 cattle from different farms were randomly sampled and tested for the presence of A. marginale between May to August 2016. The study revealed that 15.0%, 20.8% and 29.1% of sampl...

Investigation of Ehrlichia canis and Anaplasma platys from Dogs in Thailand, with Molecular Characterization and Haematological Profiles

Supawadee Piratae1*, Noraphat Khiewkham2, Nattawut Maungmungkun2, Chanakan Tippornwong2, Tossapol Seerintra2, Sirikanda Thanasuwan3, Luyen Thi Phung4

...and Anaplasma platys are rickettsial pathogens that cause infections in the blood and have adverse health effects in animals, especially dogs and other canids. PCR targeting the 16S rRNA gene was performed to determine the prevalence of ehrlichiosis and anaplasmosis in 127 dogs collected from Rayong province, Thailand. To confirm the identification of these two pathogens, PCR was performed to detect the citrate synthase (gltA) gene followed by sequencing. In a...

First Molecular Evidence of Anaplasma marginale Infection in Naturally Infected Cattle in Myanmar with Severe Hemolytic Anemia

Babi Kyi Soe¹*, Toe Win Naing², Su Lai Yee Mon¹, Nay Chi Nway³, Hiroshi Sato⁴

...e obligate intracellular rickettsial vector-borne pathogens that impact on livestock farmers with major economic constraints and eventually threaten human health in cases of zoonotic species involvement. Therefore, our study aimed to identify Anaplasma spp. infection in a bovine host using both microscopy and molecular techniques. A total of 59 samples were collected from Mingaladon township, Yangon region, and the presence of infection was assessed using a Gi...

Molecular Identification of Ixodid Tick Species and their Screening for Selected Protozoan Pathogens Collected from Large Ruminants of Azad Kashmir, Pakistan

Anisa Mushtaq¹, Murtaz ul Hasan¹*, Asim Shamim², Muhammad Ali Abdullah Shah¹, Muhmamad Arif Zafar³, Abdul Asim Farooq⁴, Aayesha Riaz¹, Muhammad Kamran¹ and Saif ur Rehman¹

... like viruses, bacteria, rickettsia and protozoa and cause mortality in humans and animals. This study was focused on morphological and molecular identification of ixodid ticks, using morphological keys and an internal transcribed spacer (ITS-2) Deoxyribonucleic acid (DNA). Moreover, the presence of Babesia and Theileria species was also investigated in ticks using 18S rRNA gene. Identification of ticks collected from 384 cattle and 384 buffaloes screened reve...

Molecular Identification and Prevalence of Blood Parasites and Rickettsia in Stray Dogs in DKI Jakarta Province

Wirokartiko Satyawardana^1,4, Umi Cahyaningsih²*, Fadjar Satrija², Safika Safika³, Arifin Budiman Nugraha²

...tify blood parasites and rickettsia in stray dogs in DKI Jakarta. It included 50 stray dogs that were statistically representative of the entire population used detect disease sampling technique from various cities across the province, where blood samples were collected and subjected to polymerase chain reaction (PCR) analysis, targeting parasites’ 18S and 16S RNA. PCR analysis showed that 19 samples were tested positive for blood parasites and