Search for Laccase

Dual Role of Multicopper Oxidase of Klebsiella pneumoniae as a Copper Homeostatic Element and a Novel Alkaline Laccase with Potential Application in Green Chemistry

Soumble Zulfiqar¹, Khuram Shehzad¹, Sana Tahir¹, Khalid A. Al-Ghanim² and Abdul Rauf Shakoori^1,2,3,*

...ve been shown to possess laccase activity. In this study, we have cloned and over-expressed the CueO of locally isolated Klebsiella pneumoniae KW strain. The CueO protein was purified to homogeneity by nickel affinity chromatography. Enzyme assays of CueO protein with phenolic substrates revealed its laccase activity. The kinetic studies showed Km value of 0.2µM, kKcat 0.68 S^-1 and Kcat/km 1.2S

Optimization of Cultural Conditions for the Treatment of Pulp and Paper Industrial Effluent by Pleurotus ostreatus (L.)

Azeem Haider¹, Muhammad Mohsin Alam²*, Azhar Ali Khan³ and Muhammad Asif Zulfiqar³

...iz; xylanase, CMCase and laccase production by Pleurotus ostreatus were optimized in shake flask cultures. The results indicated that the optimum fermentation medium in shake flask studies contained a carbon (glucose 4%), nitrogen (0.5% ammonium chloride), inoculation level (three disks, 0.5 cm in diameter), temperature (30 °C), incubation period (at 7 days) and initial pH 5.0. Under these culture conditions, the maximum level of xylanase activity (9.29 Um...

An Overview of Production and Industrial Exploitation of Bacterial Laccases

Fehmina Ashraf¹*, Muhammad Irfan², Hafiz Abdullah Shakir¹, Shaukat Ali³, Muhammad Khan¹

...al and textile etc. Both laccases and mediators have been used in different process like delignification of pulp. Laccases from bacterial species are used in dye decolouration and biobleaching processes. This review article describes the whole overview of laccases.

Emergence of Cryptococcus spp. in Donkeys in Egypt: A Potential Public Health Concern

Rahma Mohamed, Sara Nader, Dalia Hamza and Maha A. Sabry*

...ates demonstrated higher laccase (LAC1) genes among the identified virulence factors. While capsular associated protein (CAP59) gene identified alone or associated with LAC1 gene in the other 2 C. gattii isolates. This study underlines a potential association of those fungi with human disease in Egypt. In order to strengthen existing therapeutic and control approaches, further analyses of the main risk factors and the other virulenc...

Purification and Characterization of Laccase Enzyme from Locally Isolated Aspergillus flavus Strain

Syeda Rubab Zaidi* and Safdar Ali Mirza

...tion and optimization of laccase enzyme production from locally isolated and screened potential fungal strain of Aspergillus flavus was monitored. During this study culture medium and different parameters such as incubation period and inoculum size were optimized and inducers were employed for enhanced production of laccase. Partial purification was accomplished through Ammonium-Sulphate precipitation and for further purific...

Optimization of cultural conditions for enhanced production of laccase by Aspergillus flavus Maf 0139

MADIHA AFTAB¹, ARIFA TAHIR*¹, TAYYABA ASIM¹ & IRFANA MARYAM²

...echnological process for laccase production has gained great attention. Present study is concerned with the enhanced production of laccase by Aspergillus flavus Maf 0139. Five different cultural media were tested and best one was selected for laccase production. Different cultural conditions such as inoculum size, pH, temperature, incubation period, moisture content and inducer were studie...

Biologia (Lahore)

Featuring

Quantification of Sodium from Food Sources by Using Various Analytical Techniques

Nauman Jamil Khan, Rabia Tariq, Hina Saleem and Muhammad Waheed Mushtaq

Biologia (Lahore), Vol.69, Iss. 2, Pages 66-75
Studies on the Occurrence of Prostate Cancer in Gujrat, Punjab, Pakistan and its Association with Testosterone Level

Kanwal Nisa, Sadia Roshan, Shazia Shamas, Raheela Atta Mustafa, Shamaila Irum, Kalsoom Sughra and Memoona Iqbal

Biologia (Lahore), Vol.69, Iss. 2, Pages 54-60
Prevalence of Gastro-Intestinal Parasites in Pigeons of District Jhang-Pakistan

Muhammad Fiaz Qamar, Tahir Hussain, Iram Liaqat, Madiha Kiran, Abdur Rahman Ansari, Faiza Yasmeen and Tahira Batool

Biologia (Lahore), Vol.69, Iss. 2, Pages 48-53
Phytochemical Screening and In Vitro Antioxidant Activity of Extracts of Ipomoea purpurea Leaves from Iran

Fatemeh Beheshti, Maliheh Safavi, Mohammad Reza Akbari Eidgahi, Parviz Kokhaei, Mehdi Vazirian and Ali Akbar Shabani

Biologia (Lahore), Vol.69, Iss. 2, Pages 32-39