Pinar Oztopcu-Vatan1, Gokhan Kus2, Emine Inan3, Melek Gunindi Korkut3, Selda Kabadere4,* and Ruhi Uyar4
...d induces apoptosis in a hepatoma cell line (H4IIE). We determined in vitro survival rate with MTT assay, the morphological changes with an inverted microscope, apoptosis using flow cytometry and statistical significance with one way analysis of variance followed by Tukey’s multiple comparison test. IC50 values of licofelone were determined as 171 µM for 24 h and 140 µM for 48 h applications. Used as a positive control, 250 ...
Mahmoud Abdelhady Metwally1*, Mona Abdelftah Ali1, Farouk Abdelmohdy1, Mohamed Kassab1, Tarek Kamal Abouzed2 and Khalil Fathy Abou-Easa1
...edium (BMMSCs-CM) on the hepatoma cell line HepG2 and described the underlying molecular mechanisms involved. We isolated BMMSCs and identified them using flowcytometry and culture characteristics, then prepared BMMSCs-CM. HepG2 cells were treated with various concentrations of BMMSCs-CM for up to 72 h. The methylthiazolyldiphenyl-tetrazolium (MTT) assay showed decreased proliferation, while flowcytometry showed increased apoptosis and cell cycle arrest in the...
El-Tabakh, SAA l ; Abdel Wahab, KSE; Badr, AF and Helal, IG
...tal infection of a human hepatoma cell line (HepG2) at a multiplicity of infection (MOL) of one genome copy per 103 cells. Stock HepG2 cell cultures were grown in Dulbecco modified minimum essential medium (DMMM) as growth medium with 10% fetal calf serum and antibiotic mixture (IOOU penicillin and 100 mgm streptomycin/ml) and subcultured at 3-4 days. HepG2 cells were maintained throughout the experiment in Ham F-12 medium maintenance medium with 2% fetal calf...
