Search for Fugu

Molecular Cloning and Expression of Taste Receptor Gene T2R1 of Obscure Puffer, Takifugu fasciatus

Yijin He¹, Song Ma², Bo Liu^1,*, Ting Xue¹, Qunlan Zhou¹, Wu Jin¹ and Kui Chen^2,*

...">Obscure puffer, Takifugu fasciatus is a unique species of China which is distributed in the estuary of the Yangtze River, and belongs to the same genus with Red fin puffer T. rubripes which has been used as one of human genome model animal since mid-1990s. In this experiment, we cloned a pair of DNA fragment sequence including complete ORF (921bp) of taste receptor candidate gene T2R1 of T. fasciatus and studied the differential e...

The Effect of Different Preparation Methods on the Flavor of Farmed Takifugu obscurus

Zhuanxia Chen¹, Ningping Tao^1,*, Xiao Qin¹ and Dayong Liu²

...nal. The taste of Takifugu obscurus under different conditions were analyzed by sensory evaluation and electronic tongue technique. The characteristic of the taste substances were tested, such as free amino acids, nucleotides, trimethylamine oxide and betaine. Results showed that umami was the most prominent flavor. Compared with liver, IMP is the main flavor nucleotide in meat. However, the contents of the umami amino acids and betaine in liver are muc...

Studies on Bacterial Diversity and Vibrio harveyi Distribution Associated with Diseased fugu (Takifugu rubripes) in Northeastern China

Qiang Li^1,2, Guo Qiao², Li Wang¹, Jipeng Zhang¹, Ruijun Li¹, Ping Ni¹, Yi Guo¹ and Shigen Ye^1,*

...associated with diseased fugu (Takifugu rubripes) in northeastern China from January to December in 2014. The main clinical signs included fin ulceration, skin darkness, hepatohemia and intestinal hydrops. Totally, 104 diseased live fish were collected and 70 strains isolated from naturally diseased T. rubripes. Most isolates were obtained in May, September and December. The isolates were identified through 16S...

In Silico Analysis of Cu-Zn Superoxide Dismutase and Mn Superoxide Dismutase Genes in Fugu (Takifugu rubripes)

Mehtap Bayir*

...and sod2 genes in fugu—a model organism—were determined. Maximum amino acid similarity identity was found between putative fugu Sod1 and Sod2 proteins and their orthologs from teleost fish and tetrapods. Phylogenetic clustering was seen between sod genes in fugu and their orthologs. Finally, highly conserved gene synteny was determined between

Characterization of CLCN5 and Expression Profile under Low-Salinity Stress in Takifugu rubripes

Lin Ye, Jie-Lan Jiang*, Jia Xu, Rui-Ting Liu, Yin-Qiu Tian, Qing-Qing Zang, Jia-Yi Cao and Ming-Guang Mao*

...CN5) was cloned from Takifugu rubripes using the reverse transcription PCR (RT-PCR) technique. The results showed that TrCLCN5 cDNA contained a complete open reading frame of 2505 bp continuously encoding 834 amino acid residues. Sequence alignments revealed that the TrCLCN5 cDNA is highly homologous (96.2% and 96.1%) with that of Takifugu bimaculatus and Takifugu flavidus. SignalP and TMH...