Habib-ur-Rehman1, Masood Ahmed Siddiqui1,*, Abdul Qayyum1, Arifa Bano1 and Naeem Rashid2
...ullulanase also known as amylopullulanase hydrolyzes α-1,6 glycosidic bonds in pullulan and α-1,6 as well as α-1,4 glycosidic bonds in starch. Most of the previously reported type II pullulanases are metal ion dependent and hard to purify, to homogeneity, due to low level of expression or the proteolytic machinery of the host. Herein we report expression in Escherichia coli and purification of metal ion independent type II pullulanase ...
