Articles

Purification, Serology and Prevalence of Broad Bean True Mosaic Comovirus (BBTMV)

Khatab, Eman A.H, Zein, Salwa N, Ahmed, Amal A

Broad bean true mosaic virus was purified from infected faba bean plants. The UV absorption spectrum had a maximum at 260 nm and a minimum at 240m. The ratios of Amax /A min and A 260/A 280 were 1.48 and 1.71, respectively. The yield of purified BBTMV preparat…

Innovation of Indoor Real-Time Polymerase Chain Reaction for Diagnosis of Camel Pox Virus in Clinical Field Samples Using Primer Site Belongs to Capripoxvirus

SHARAW11'2*S S. S. A, AL-HOFUFY, A. N, AL-BLOW, M. H

Real-time amplificationtechniques are currently used to determine the viral nucleic acid (NA) in clinical samples for diagnostic purposes. In disease management contexts, until now, a little have been described for the molecular detection of camel pox virus (C…

Evaluation of Tip Culture and Thermotherapy for Elimination of Carnation Latent Virus and Carnation Vein Mottle Virus from Carnation Plants

Ahmed, Amal A, Khatab, Eman A.H, Dawood, Rehab A et al.

Carnation latent virus (CLV) and Carnation Vein Mottle Virus ( CarVMV ) were of the most important virus affecting carnation plants .The two viruses were detected serologically by DAS-ELISA using specific antibodies . Shoots from plants infected with ( CLV &am…

Antiserum Production and Control Measures of Pepper Mottle Potyvirus using Different Applications

El.-Kadyl, M.A.S, Badr, A.B, zein, Saiwa N et al.

Pepper mottle virus (PepMoV) was purified with a high degree of purity and sufficient quantities for its antiserum production. The absorption spectrum of the purified virus had a min at 245nm and a max at 260 nm. The A260/280, A280/260 and Amax/min ratios were…

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Chemical Mutation Induction of In Vitro Cultured Shoot Tip of Banana cv. Grand Nain for Resistance Some Viruses Diseases

El-Sayed • l, Eman H, Mahfouze, Sherin A, Shaltout, A. D et al.

The chemical mutagens have become important tool to enhance agronomic fraits of banana crop. It is being used to produce virus resistance in various susceptible banana crop to improve it yield and quality traits against BBTV and BMV viruses. There are several…

Double Infections with Cucumber Mosaic Virus and Plum Pox Virus (SHARKA) in Apricot Trees

Ahmed, Amal A, Fath-Allah, Mervat M

Cucumber mosaic virus(CMV) and Plum pox virus(PPV) (Sharka) were isolated from naturally infected apricot (Prunus armeniaca cv. ElAmar) trees showing leaf mottling, marginal leaf necrosis deformation and abortive flowers grown at Kalubia and el-Fayoum Governor…

Characterization of Tobacco Mosaic Tobamovirus (TMV-S ) Isolated from Sunflower (Helianthus annuusl.) in Egypt

Zein Salwa N, Abd El-khalik, Samaa, Khatab, Eman A.A.H, Azzam et al.

This is the first record of Tobacco mosaic Tobamovirus (TMV-S) on sunflower in Egypt. It was originally isolated from naturally infected sunflower plants growing in Giza Res. Station, showing systemic mosaic and spots. Purified TMV-S migrated as a single zone…

Immune Response of Pregnant Mares and Their Foals for Inactivated Equine Influenza Vaccine

Ebied, Eman M, Salem, Zeinab T, Al-Imam, Hemmat S

This study comprises the immune response of pregnant mares and their foals to monovalent inactivated freeze-dried equine influenza (El) vaccine, reconstituted in diethylaminoethyle (DEAE) Dextran solution as an adjuvant. It was found that mares developed high…

Nucleotied Sequencing and Phylogenic Analysis of Fusion (F) Epitope for Egyptian Pestes Des Petit Ruminants Virus (PPRV) Predicting Unique Criteria Stated as Egypt 2009

Sharawil, S.S.A, Abd El-Rahim, I.H.A.•

Peste des petit ruminants virus (Abd El-Rahim2) was previously isolated and serologically identified from suspected outbreaks of PPR among sheep and goats in Qalyubia Province, Egypt at 2006. In this study six of this PPRV isolates were confirmed by reverse tr…

Comparative Epidemiology of Infection with Human Cytomegalovirus in Cairo and South London

Abdel Hamidl, Saiwa El-s, Abdel-Wahab l, Kouka S.E, salehl, Laila H et al.

Human cytomegalovirus (HCMV) infection induces HCMV IgM antibodies in acute and HCMV IgG antibodies in convalescent phases while HCMV IgG antibodies last for years. Testing for HCMV IgG antibodies as an age-related pattern of infection was studied in Cairo and…

An Egyptian Study of Mother to Child Transmission of Hepatitis C Virus

Abo Elmagd l, Enas K, Abdel-Wahab l, Kouka S, Alrasheedy l, Zeinab E et al.

This study aims to assess markers of hepatitis C Virus (HCV) including HCV antigen detection in peripheral blood mononuclear cell (PBMNC) lysates, antibodies to HCV core, NS3, NS4 and NS5 epitopes by enzyme linked immunoassay (ELISA) in mothers and their infan…

Comparison of Rapid Antigen Test with Reverse Transcription Polymerase Chain Reaction for Diagnosis of Respiratory Syncytial Virus Infection in Infants and Young Children

Abo-Elmagdl, Enas K, El-Mougy, Hala M. T, Abd El-Salam, Manal

Respiratory syncytial virus (RSV) is the most common cause of hospitalization for acute lower respiratory tract infection, specifically bronchiolitis, affecting infants and young children. An association between primary RSV infection and childhood asthma has b…

Incidence of Fig Leaf Mottle-Associated Virus and fig Mosaic Virus in Eastern Province of Saudi Arabia

Alhudaib, Khalid

Fig plant, Ficus carica L., is grown in Saudi Arabia and is being affected by fig plant mosaic diseases (Fig leaf mottle-associated virus, Fig mosaic virus). The main symptoms are chlorotic mottling, blotching and various types of leaf deformation. Samples wer…

Characterization of Celery Mosaic Virus Isolated from Some Apiaceae Plants

Ahmed, AmalA, Zein, Salwa N, Khatab, Eman A. H

Celery mosaic Potyviruses (CeMV) was detected and isolated from naturally infected celery plants showing mosaic symptoms suspected to be caused by viral infection, also, it was isolated from other hosts. The isolated virus was biologically purified from single…

Biological and Molecular Characterization of Potato Infecting Alfalfa Mosaic Virus in Egypt

El-Helaly, Sahar H, Ahmed, Amal A, Awad, M.A et al.

Alfalfa mosaic virus (AMV) was isolated from naturally infected potato (Solanum tuberosum, L. cv. Spunta) plants, showing bright yellow blotching or mottling «Calico» and aucuba symptoms suspected to be due to virus infection. Leaf samples were col…

Three Viroids Frequency Naturally Infecting Grapevine in Egypt

Nasr-Eldin, M. A, El-Dougdoug, K. A, Othman, B. Ahmedi et al.

Three viroids: Citrus exocorlis viroid (CEVd), Hop stunt viroid (HSVd), and Potato spindle tuber viroid (PSTVd) can be detected in naturally infected grapevine by biological indexing and molecular methods. In the present study, direct tissue-printing and dot-b…

Partially Nucleotide Sequence and Secondary Structure of Chrysanthemum Stunt Viroid Egyptian Isolate from Infected Chrysanthemum Plants

El-Dougdoug, Kh.A, Rezk, A.A, Dawoud, Rehab A et al.

Chrysanthemum stunt viroid Egyptian isolate (CSVd-EG) was isolated from infected Chrysanthemum plants. It is a member of Pospiviroidae. In order to study the structure of CSVd-EG, it was reverse transcribed in total RNA from infected leaves and then amplified…

Impact of Polyethylene Glycol on Newly Established Ovarian Cell Line (Sl-Omi) from Leaf Worm of Spodoptera Littoral's and on Viral Infection

Khamiss l, Omaima, El Helaly, Alexandra, Abol Ela, Said

New cell line from the Egyptian cotton leaf worm ( Spodoptera littoralis ) originated from the insect ovaries tissues was established, cloned and maintained as contentious cell line. Nine serial dilutions of Polyethylene glycol (PEG) were prepared from mother…

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