The chemical mutagens have become important tool to enhance agronomic fraits of banana crop. It is being used to produce virus resistance in various susceptible banana crop to improve it yield and quality traits against BBTV and BMV viruses. There are several mutagens available for banana crop improvement and each mutagen has its important role as positive or negative effects on banana plants. The chemical mutagens such as create mutations in the genome of plants. Selection of plant mutants is based on morphological and ISSR-PCR markers. The DNA based marker is reliable and reproducible for mutant selection for BBTV and BMV resistance banana plants used in the study. Explants from shoot apical meristem were cultured on MS-medium supplemented with different concentrations of 2, 4-D (2, 4 and 6 mg/l) 6-Benzylaminopurine (6, 7 and 8 mg/l), and Sodium Aide ranging from (1, 2 and 3 mg/l) for induction of mutation. In vitro screened plants after rooting were hardened and acclimatized in the glass house and were shifted into the field. Field screening was carried out against two different isolates of (BBTV and BMV) by using syringe method of inoculation. It was observed that, only two banana plants of the concentration 6 mg/l from 2, 4-D was found to be resistant against BMV isolate. In the same time, it is not BBTV resistance banana plants