Articles
12 open-access articles
Research ArticleEvaluation of Novel Anti-VEGF Molecules in the Animal Model of Human Lung Cancer
Sanjukta Chakrabarti, Colin J. Barrow, Rupinder K. Kanwar, Venkata Ramana, Rakesh N. Veedu, Jagat R. Kanwar
Abnormal angiogenesis or the formation of aberrant vasculature in humans, may lead to various pathological conditions including tumor development. The most important factor implicated in the angiogenic processes is vascular endothelial growth factor (VEGF) pro…
4 Nov 2017
ReviewFeasibility of Anti-Idiotypic Aptamers to Detect Rickettsia Exposure
John G. Bruno
Twenty-seven DNA aptamers were developed against the Fab fragments of murine antibodies developed to bind Rickettsia rickettsii and Rickettsia typhi. A feasibility study was conducted to generate anti-idiotypic aptamers against Fab fragments of these antibodie…
5 Jun 2017
Research ArticleEvaluation of the Ability of Anti-Lipopolysaccharide Aptamers to Discriminate Various Pathogenic E. coli Bacteria
John G. Bruno
Lengthy 200 base DNA aptamers were developed against extracted lipopolysaccharides (LPS) of E. coli O157 and the so called “Big six” non-O157 Shiga-toxin producing E. coli (STEC) serovars (O26, O45, O103, O111, O121 and O145). These aptamers were a…
23 Jan 2017
ReviewRecent Advances in Aptamer Based Biosensors for Detection of Antibiotic Residues
Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand
Antibiotics are widely used as bacteriostatic or bactericidal agents either to kill or inhibit the growth of microorganisms. Their abuse results in various side effects on the human health, environment and agriculture. The occurrence of bacterial “suprai…
22 Nov 2016
ReviewThe Point behind Translation of Aptamers for Point of Care Diagnostics
Tarun Kumar Sharma, John G. Bruno, William C. Cho
Owing to the high affinity and specificity in recent years aptamers, have emerged as competitive tools with the potential to replace antibodies in many diagnostic formats. The aptamer industry is continuously growing to create space in research and diagnostics…
22 Oct 2016
Research ArticleAptamer
John G. Bruno, Jeffery C. Sivils
Western blot-like analyses using DNA aptamers raised against the surfaces of general nonpathogenic Escherichia coli (ATCC No. 8739) as well as alpha and gamma intimins and Shiga-like toxin 2 (SLT-2) B subunit verified that the aptamers bound proteins of…
1 Sept 2016
ReviewWhole Cell SELEX Technology and its Applications
Neha Singh, Anjana Pandey
SELEX is an in vitro selection procedure to evolve single-stranded nucleic acids known as aptamers. Aptamers are known for their high selectivity and specificity and their ability to form 3D conformations. CELL SELEX is a modification of SELEX where inst…
30 Aug 2016
Research ArticlePreliminary Development of an Enzyme-Linked Fluorescent DNA Aptamer-Magnetic Bead Sandwich Assay for Sensitive Detection of Rickettsia Cells
John G. Bruno, Chien-Chung Chao, Zhiwen Zhang, Wei-Mei Ching, Taylor Phillips, Allison Edge et al.
Abstract | DNA aptamers were developed against Rickettsia typhi (R. typhi) whole cells and individual candidate aptamer sequences were ranked according to affinity by an ELISA-like microplate-screening assay (ELASA). Top three candidate aptamers were then pair…
2 May 2016
CommentaryAutomated Aptamer Development May Represent the Last and Best Line of Defense against Proverbial
John G. Bruno
This commentary addresses the perceived need for automated robotic development of aptamers or aptamer conjugates with associated mass production to provide rapid passive immunity for large human populations in the event of a major pandemic or infectiou…
2 May 2016- Commentary
Aptamers as Viral Replication Antagonists
Nicola J. Stonehouse
Aptamers are nucleic acids, selected in vitro to specifically bind target molecules. They can therefore be useful as molecular tools and also as novel therapeutic agents. This commentary describes RNA aptamers selected to bind to the RNA-dependent-RNA polymera…
13 Sept 2014 
ReviewAptamers as an Emerging Player in Biology
Aradhana Chopra, Ravi Shukla, Tarun Kumar Sharma
Aptamers are single stranded nucleic acid molecules that are considered as chemical rival of antibodies. They are generated through an in vitro evolution process called SELEX. These single stranded nucleic acid aptamers can acquire various secondary and tertia…
25 Aug 2014- Commentary
Aptamers and SELEX: Tools for the Development of Transformative Molecular Recognition Technology
Maureen McKeague, Maria C. DeRosa
Aptamers, single stranded nucleic acid-based molecular recognition elements, are generated through an in vitro evolution process called SELEX. The selection of aptamers with optimal specificity and high affinity remains a challenge, leading to the recent emerg…
25 Aug 2014