Issue · 2004
2004
29 articles · All issues →
Research ArticleOccurrence of Rose Colour Break Tobamovirus in Egypt
Amal Abo El-Ela Ahmed, Eman A.H. Khatab, M.S. Shafie
Rose colour break virus was detected in naturally infected rose growing in Orman Garden for ornamental plants, Giza Governorate and it was found to be widely spread in rose fields. The virus was transmitted mechanically and by grafting (chipbudding) but not by…
5 Jan 2024- Research Article
Detection and Characterization of Apple Chlorotic Leaf Spot Virus (ACLSV) Isolate from peach orchards in Egypt
A.A.Farrag, I.A.M. Ibrahim, H.M. Mazyad
Apple Chlorotic Leaf Spot virus (ACLSV) was isolated from symptomless peach trees and then identified with a specific antiserum (Sanofi comp) using Double Antibody Sandwich ELISA (DAS-ELISA) and Dot Immunobinding Assay (DIBA). Survey was carried out during 200…
5 Jan 2024 - Research Article
Virus Elimination from Infected Garlic Plants Using Different Techniques
S.A. Sidaros, R.A. Omar, S.A. El-Kewey l, Samaa Abd El-Khalik
Production of garlic virus free plant was attempted by means of chemotherapy, meristem-tip culture, and thermotherapy techniques. Three different meristem sizes (5, 3 and 1 mm long) were excised from three garlic cultivars (Chinese. Italian and Balady). Merist…
5 Jan 2024 - Research Article
Physiological and Histopathological Changes in Potato Virus X and Potato virus Y- Infected Potato Plants
Hanaa H.A.Gomaa, A. F. Moustafal, Kh.A.El-Dougdoug, A.A. Abou-Zeid, S. Y.M. Mahmoud
Potato viruses X and Y were used for study the effect of the two viruses on the metabolism of potato plants. It was found that detectable decrease or dry matter, total nitrogen, tuber Starch, number of starch granules, and total protein. The nucleic acid conte…
5 Jan 2024 - Research Article
Standardization and Optimization of a Long-Distance PCR Assay for Foot-and-Mouth Disease Virus
M.E. Shawky, A.M. Daoud
Taq DNA polymerase with different concentrations of other thermostable DNA polymerases with 3' to 5' exonuclease activity (PFU and Vent) was used in the amplification of large fragments (2 Kb, 3 CD genes) of foot and mouth disease virus (FMDV). Such long-dista…
5 Jan 2024 - Research Article
Alterations of Immunologic Responses of Calves Experimentally Infected with Virulent Either Type I or 2 Non-Cytopathogenic Strains of Bovine Viral Diarrhea Virus
Ahmed Abd El-Samie H. Ali
Bovine viral diarrhea virus (BVDV) is a worldwide pathogen infecting cattle. Eleven Holstein calves between 6-8 months of age that were seronegative to BVDV were divided into three groups (n=4 for NY-1, n=4 for 890 and n=3 control). Fibroblast cells were prepa…
5 Jan 2024 - Research Article
Persistence of Maternal Antibodies in Calves Born to Dams Naturally Infected with Bovine Ephemeral Fever Virus
S. A. Khalil, M. El-Sayed, M. M. El-Fayomy, H. Y. Hassan, A. Zaghawa
Bovine ephemeral fever virus (BEFV) is an arthropod born rhabdovirus which causes disabling febrile infection among cattle and water buffalo. The disease is characterized by inflammation of mesodermal tissue as achieved by muscular shivering, stiffness, lamene…
5 Jan 2024 - Research Article
Preparation and Evaluation of Anti-Rabbit IgY Peroxidase and FITC Conjugates
N. A. Sherif, Lamiaa M. Omar, S. M. Shafei, Elham A. El-Ebiary
In this investigation, a trial for preparation of anti-rabbit immunoglobulin-Y IgY peroxidase and fluorescein isothiocyanate (FITC) conjugates was conducted. Rabbit IgG was separated and injected into specific pathogen free (SPF) laying hens, from which their…
5 Jan 2024 - Research Article
Isolation, Antigenic and Molecular Characterization of Bovine Viral Diarrhea Virus Field Strains from Apparently Health Buffaloes in Egypt
Nahed A. Mohamed l, H. A. Hussein, Fathia M. Mohamed, M. A. Shalaby
In the present study, we report the isolation of BVDV field strains from apparently health buffaloes. One hundred and thirty-two samples including buffy coat and organs were collected from apparently health Screening buffaloes in Cairo, Mansoura and Suez gover…
5 Jan 2024 - Research Article
Population Fluctuations of Aphids in Relation to Virus Disease Incidence in Egyptian Faba Bean Fields
Mohga M. El-Tahlawey, A. Mandour, AE Aboulata
Infection induced by both Faba bean necrotic virus (FBNYV) and mosaic causing viruses were inspected during Oct. 2003 to Apr. 2004 on Faba bean fields grown in Qalyubia governorate. Population density of seven different aphid species on faba bean was arranged…
5 Jan 2024 - Research Article
Occurrence and Spread of Sugar Beet Rhizomania Disease Caused by Beet Necrotic Yellow Vein Benyvirus in Some Governorates of Egypt
S.Y.M. Mahmoud, M. Hashem
Beet necrotic yellow vein Benyvirus (BNYVV) is the important soilborne virus disease in the production areas of sugar beet. In this study. field survey was carried out in sugar beet growing areas belonging to Kafr-ElSheikh, El-Beheira, El-Dakahlia, El-Gharbia.…
5 Jan 2024 - Research Article
Faba bean necrotic yellows virus (FBNYV) in Egypt: Characterization and Virus-Vector Relationship
A. E. Aboul-Ata, Mohga, M. El-Tahlawey, M. A. Amer, A.M. Mandour
Faba bean necrotic yellows virus (FBNYV) has been isolated and identified as an isolate of FBNYV from Egypt. FBNYV characterization was proved according to the following Criteria: Symptomatology, insect transmission (Aphis craccivora), serological tests (DAS-E…
5 Jan 2024 - Research Article
Isolation and Characterization of Peach Rosette Mosaic Virus (PRMV) in Egypt
A. A. Kheder, I. A. M. Ibrahim, H. M. Mazyadl
Peach rosette mosaic virus (PRMV) was detected from naturally infected commercial peach trees growing under field conditions at El Dakahlia Governorate, using DAS-ELISA. The detected virus is recorded for the first time in Egypt. Virus identification was perfo…
5 Jan 2024 - Research Article
A New Whitefly Transmitted Geminivirus Infecting Tomato Plants in Egypt
A-New-Whitefly-Transmitted-Geminivirus
Whitefly transmitted Geminiviruses cause severe damage to different crops in Egypt. Geminiviruses often occur in mixed infections, and it is difficult to diagnose such mixtures by biological properties. Two types of yellow leaf curl symptoms were collected fro…
5 Jan 2024 - Research Article
Induction of Salicylic Acid in Cucumber against ZYMV Potyvirus by some Nutrient Chemicals
M.A. Abo-EInasr l, Kh.A. EL-Dougdougl, M.H. El-Kattan, L.A. Salem
Systemic acquired resistance (SAR) could be induced in cucumber plants using different concentrations of seven nutrient chemicals against Zucchini yellow mosaic Potyvirus (ZYMV). These chemicals were. Potassium sulfate, Magnesium sulfate, Ammonium sulfate, Che…
5 Jan 2024 - Research Article
The Influence of Gibberellic Acid (GA) and Indole Acetic Acid (IAA) on The Production of Local Lesions on Nicotiana sylvestris Plants Infected with Tomato Mosaic Tobamovirus (ToMV)
E.F. Mohamed, I.M. Sabra
Tomato mosaic disease caused by Tomato mosaic (ToMV) is widespread wherever tomato is grown ToMV affects plants and yields. Virus was isolated and identified on the base of symptomatology on tomato plants, host range, diagnostic hosts, physical ptoperties, mod…
5 Jan 2024 - Research Article
An Inhibitory Activity of Propolis Extract against Faba Bean Mottle Bromovirus (BBMV)
E.F. Mohamedl, A.A. Owayss
A Bromovirus was isolated from naturally infected faba bean plants in Fayoum Governorate. The virus was identified as Faba bean mottle Virus (BBMV) according to symptomatology on Faba bean plants, host range, diagnostic hosts, virus stability and ELISA detecti…
5 Jan 2024 - Research Article
Histo-cytopathological Effects of Cucumber Mosaic Cucumovirus on Squash leaves
M. Osman, Kh. El-Dougdoug, E. T. Abd El-Salam, R.M. Taha, R.M. El-Hamidl
An isolate of Cucumber mosaic cucumovirus (CMV) infected cucumber plants was detected by DAS-ELISA and confinned by inoculation of Chenopodium amaranticolor. CMV-isolate was propagated on squash plants var. Eskandarani. After 15 days. systemic symptoms appeare…
5 Jan 2024 - Research Article
Interaction Between Rhizobium Meliloti and Its Specific Viruses in Clover Plants
B. A. Othman, Kh. A. El-Dougdoug, M. H. Abdel Ghaffar, T. F. El-Arabi
Four phages singed as RM1, RM2, RM3, and RM4 specific for Rhizobium meliloti (R meliloti) were isolated from tested rhizosphere soil samples collected from Qalyubia and North Sinai Governorates based on plaque morphology, The extinction spectra of purified pre…
5 Jan 2024 - Research Article
Production of FMDV ELISA Kit for Antibodies Detection in Animals Using Native Strain with Production of Reference Hyper-immune Serum in Guinea Pigs
M. E. Shawky, A. M. Daoud
The objective of the present study was to evaluate an enzyme linked immunosorbent assay (ELISA) based kit for detecting foot and mouth disease (FMD) antibodies using a native FMDV strain (O1. Aga 93). Results were compared to those of serum neutralization test…
5 Jan 2024 - Research Article
Molecular Characterization of Hyper Variable Region of the Classical and Very Virulent Egyptian Isolates of IBDV
Seham A. El-Zeedy, Dalia A. Abd El-Moaty, H. A. Hussein, M. A. Shalaby
Many recent outbreaks of Infectious bursal disease in commercial chicken flocks worldwide are due to the spread of very virulent IBDV. Two Egyptian isolates Of IBDV isolated from Giza (Giza/2000) and Qalyubia (Kal/2001) governorates were characterized by RT/PC…
5 Jan 2024 - Research Article
Phylogenetic Characterization of Some Bovine viral diarrhea viruses in Egypt
A. A. El-Kholy, S. Vilcek, A. M. Daoud
Sixty-seven buffy coat specimens, obtained from clinically suspected cattle at farms located in Belbees. El-Sharquia, were screened for BVDV. Only 9/67 of the tested specimens (13%) were BVDV positive by reverse transcription-polymerase chain reaction (RT-PCR)…
5 Jan 2024 - Research Article
Cytopathogenicity of Local Field Strains of Bovine Viral Diarrhea Virus Isolated from Apparently Health Buffaloes in Egypt
H. A. Hussein, N.A. Mohamed, F.M. Mohamed, M.A. Shalaby
The growth behavior of BVDV field strains isolated from apparently health buffaloes in cell culture was studied. The isolated viruses were classified into 3 groups based on the onset and severity of cytopathic effect (CPE). The cytopathogenicity of the isolate…
5 Jan 2024 - Research Article
Antigenic Characterization and Pathogenicity Study of Recent Field Isolates of Infectious Bursal Disease Viruses
H.A. SULTANl, H.A. HUSSEIN, F.F. EL-KHAYAT
Epidemiological investigation of severe outbreaks of infectious bursal disease (IBD) among vaccinated chicken flocks during the period 2002-2003 revealed that all IBDV outbreaks occurred at 18-33 days of age. 70% (21/30) of these outbreaks characterized…
5 Jan 2024 - Research Article
Detection of BVDV Associated with Mortalities in Camels
M.R. Abd-El Wahab l, H.A. Hussein, T.M. Asfourl, M.A. Shalaby
BVDV was detected in tissues collected from dead camel cases using antigen capture ELISA and fluorescent antibody assay. Histopathology examination of different organs revealed changes similar to those reported with BVDV infection. The existence of BVDV in ser…
5 Jan 2024 - Research Article
Bovine Leukosis in Holstein Cattle in Saudi Arabia as Evidenced by Presence of Specific Antibodies
Musaad A. Al-Dubaib
In the present study, serum samples obtained from Holstein cattle, native cattle, sheep, goats, and camels in Al-Qassim, and surrounding regions were tested for bovine leukemia virus (BLV) antibodies for different animal species. Agar gel immunodiffusion was c…
5 Jan 2024 - Research Article
Attenuated Rift Valley Fever Virus (NMP18) Chemically for Vaccine Development
M.S. Wassel, Elham A. El-Ebiary, Soliman, Y.A.l, El-Sayed, M.M
Serial treatment of RVF virus ZH501 with nitrous acid and 5-flourouracil has been employed to derive an attenuated strain of Rift valley Fever virus (NMP18) for use as alive virus vaccine besides studying its safety, potency, and the duration of immunity in pr…
5 Jan 2024 - Research Article
Preparation and Evaluation of Anti Rota IgG Conjugated with Fluorescein
Arwa H. El -Naggar, Nirmeen G. Shafiek, M. S. Wassel
Hyper immune serum against bovine Rota was prepared in guinea pigs through its inoculation with complete and incomplete Freund's adjuvant and without adjuvant in successive doses into susceptible guinea pigs. Serum neutralization (SNT), ELISA and indirect fluo…
5 Jan 2024 - Research Article
Infection with the Non cytopathic BVDV-2 Strain 890 Prevents Replication of Superinfecting Cytopathic BVDV-I RNA in BT and MDOK Cells
Ausama A. Yousif, Sandy M. Watkins, Lyle J. Braunl, David J. Hurley, Christopher C. L. Chase
Reports of nonhomologous recombination-events between persistently infecting and vaccinal strains of Bovine virus diarrhea virus (BVDV) indicate that mixed pestivirus infections occur in vivo and that bovine cells support replication of RNA from BVDV-I and BVD…
5 Jan 2024