Ameliorative Effect of Alpha Lipoic Acid on Manganese-Induced Testicular Dysfunction
Ameliorative Effect of Alpha Lipoic Acid on Manganese-Induced Testicular Dysfunction
Wassan Mhammed Husain1* and Raghad Raad Hwidyii2 and Ali Mosa Rashid Al-Yasari3
Effect of Mn, ALA, and Mn +ALA on testosterone concentration (A), LH (B), and FSH (C) concentration (mIU/ml) in rats at on day 0,28, and 56) the experiment. Values represented as M ±SE, n = 6 rats. ns: (P>0.05), ** (P<0.05), ***(P<0.001), ****(P<0.0001).
The effect of Mn, ALA, and Mn +ALA on MDA (A), serum glutathione peroxidase (B) and total antioxidant capacity (TAC) (C) (nmol/ml) in rats on days 0, 28, and 56 days of experiment. Values represented as M ±SE, n = 6 rats. ns: (P>0.05), ** (P<0.05), ***(P<0.001), **** (P<0.0001).
Effect of Mn, ALA and Mn +ALA on sperm count (A), sperm motility (B), sperm abnormality (C) and dead sperm (%) (D) in adult male rats at 56 day of experiment. Values represented as M ±SE, n = 6 rats. ns: (P>0.05), ** (P<0.05), *** (P<0.001), **** (P<0.0001).
Histopathological section of testicular tissue, (A) section of testes from control group, showed normal structure and germinal cell. (B) section of testes from rats treated with (Mn), ( ) demonstrate the severe atrophic necrosis. (C) section of testes from rats treated with (ALA) showed ( ) a seminiferous tubule are filled with sperms, and the phases of spermatogenesis are visible. (D) section of testes from rats treated with (Mn + ALA) showed regular structure of testes and the seminiferous tubule filled with sperm () and milled congestion of blood vessel () (100 X).
