Studies were carried out on thymol as a controlling agent -1 for bee mites. In this study thymol powder (5mg hive ) was compared with formic acid 65 % and fluvalinate (strip) as standard control measure against Varroa mite. The mite mortality rate was recorded in honeybee colonies of Apis mellifera during 2011-12 at research farm of Beekeeping and Hill Fruit Pests Research Station, Rawalpindi. Analysis of variance for different treatments indicated (F=3.64, F>P=0.15) significantly different effect among treatments as well as for the control of mite. The average efficacy of thymol was 73.72% as compared to 69.21% by fluvalinate, 72.23% by formic acid and 13.1% in control. The reduction in the effectiveness of fluvalinate may be attributed to the development of resistance against fluvalinate strip.