Mohamad Nor Roslindawani1, Adilah Shahridon Syafiqah1, Faez Firdaus Abdullah Jesse1, Abdul Wahid Effendy2, Mohammad Zamri-Saad1*

1Universiti Putra Malaysia; 2Universiti Malaysia Terengganu, Malaysia.

*Correspondence | Mohammad Zamri-Saad, Universiti Putra Malaysia; Email: mzamri@upm.edu.my

Abstract
Caseous lymphadenitis (CLA) is a chronic contagious disease of sheep and goats worldwide. It is caused by a bacterium known as Corynebacterium pseudotuberculosis and vaccination is the most suitable method of control. This study measures the humoral and cell-mediated immune responses following vaccination with a recombinant CLA vaccine with palm-based oil as adjuvant. Twenty-five adult rats were divided into 5 groups. Group 1 was vaccinated intramuscularly with recombinant CLA vaccine without adjuvant, groups 2, 3 and 4 were vaccinated with the same vaccine containing 3%, 5% and 7% of palm-based oil, respectively while groups 5 was injected with PBS. The immunoglobulin and cell-mediated immune status were measured for a period of 10 weeks. Rats vaccinated with recombinant vaccine without adjuvant showed slightly high but short response of antibody, CD4+ and CD8+. Rats vaccinated with the recombinant vaccine containing 3% palm oil adjuvant showed significantly (p<0.05) highest and lasting antibody levels and percentages of CD4+ and CD8+ cells. Hence, it is concluded that recombinant CLA vaccine with 3% palm oil adjuvant has potential to be developed as a CLA vaccine.

Keywords | Caseous lymphadenitis, Palm oil, Recombinant vaccine

Editor | Asghar Ali Kamboh, Sindh Agriculture University, Tandojam, Pakistan.

Received | November 17, 2015; Revised | December 10, 2015; Accepted | December 12, 2015; Published | January 08, 2016

Citation | Roslindawani MN, Syafiqah AS, Jesse FFA, Effendy AW, Zamri-Saad M (2016). Recombinant caseous lymphadenitis vaccine with palm oil as adjuvant enhances the humoral and cell-mediated immune responses in rat model. J. Anim. Health Prod. 4(1): 22-25

ISSN | 2308–2801

Copyright © 2016 Roslindawani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.