Molecular detection of DNA component 6 (DNA-N) of banana bunchy top virus isolated from Egypt

Banana bunchy top virus (BBTV) is considered the most serious disease

affecting banana in Egypt.BBTV was isolated from infected banana(Musa

acuminata cv Grandnain)obtained from EL-Behira, Egypt

byPentalonianigronervosa .Polymerase chain reaction (PCR) was used to detect

BBTV in infected plants and banana aphid (P.nigronervosa) using specific primer

of DNA-1 for BBTV. The results showed that amplified PCR product with the

expected size 476 bp for both infected banana and aphid. PCR was used to detect

component 6 of BBTV-DNA using specific primer at expected size 813 bp.The

isolated component was cloned into PCR™-4-TOPO vector (3.956~kb) and were

transformed using minipreperation method. Component-6 was sequenced to

determine the phylogenetic with other published isolates. Phylogenetic tree showed

the identity percentage of Egyptian isolate group of BBTV-component- 6 with

component 6 of Indian, Taiwanese and Pakistani isolates group was 97%, whereas

it was 84% in case ofChinese isolate group.

Abstract