An epidemic study was conducted on weed plants for the primary investigation of natural enemies which may became promising biocontrol agents. A total of 7 weed plants include Commelina benghalensis, Cyperus rotundus, Crotalaria verrucosa, Digera muricata, Sida cordifolia, Ipomoea pestigridis and Trianthema portulacastrum were selected for in vitro studies. Fungal isolates namely Ascochyta cypericola, Bipolaris sp., Chaetomium globosum, Colletotrichum capsici, Colletotrichum sp., Curvularia lunata, Curvularia tuberculata, Fusarium oxysporum, Helminthosporium sp. and Gibbago trianthemae were isolated by inoculation of parasitized weed propagules like infected leaves, stems, root and floral parts on different fungal growth media. The diagnostic characteristic features of each isolate were critically studied using relevant literature. The pathogenicity of the isolates was examined to select biocontrol agent against host weed.