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Short Communication · Volume 3, Issue 2, 17 May 2016 · Open Access

Genetic Characterization of Pigeon Paramyxovirus Type 1 in Egypt

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Affiliations & Notes ⌄
  1. 1Department of Virology, Faculty of Veterinary Medicine, Cairo University, Egypt
  2. 2Department of Virology, Faculty of Veterinary Medicine, University of Sadat City, Egypt
  3. 3Central Laboratory for Evaluation of Veterinary Biologics, Egypt
  4. 4The Pirbright Institute, Ash Road, Pirbright, Woking, GU24 0NF, United Kingdom
Article Info ⌄
Journal:
Hosts and Viruses
Published:
17 May 2016
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From the article

  • Abstract | This study reports the first pathotypic and genetic characterizations of the pigeon paramyxovirus type I (PPMV-1) from pigeon in Egypt during 2014.
  • Pathotyping, based on the mean death time (86 hours) in eggs and the intracerebral pathogenicity index (1.2), and the amino acid motif, (112KRQKR116), at the cleavage site of the fusion (F) protein indicated the emergence of PPMV-1 in Egypt.
  • Phylogenetic analysis of the F and haemagglutinin-neuraminidase (HN) genes indicated that the isolate clustered with the PPMV-1 strains recently reported from Israel within subgenotype VIb.
  • Phylogeny indicated that the isolate, Pigeon/Egypt/VRLCU/2014, clustered with the APMV-1 strains within genotype VI of class II (Figure 2).

Selected from the article's own text.

Abstract

This study reports the first pathotypic and genetic characterizations of the pigeon paramyxovirus type I (PPMV-1) from pigeon in Egypt during 2014. Pathotyping, based on the mean death time (86 hours) in eggs and the intracerebral pathogenicity index (1.2), and the amino acid motif, (112KRQKR116), at the cleavage site of the fusion (F) protein indicated the emergence of PPMV-1 in Egypt. Phylogenetic analysis of the F and haemagglutinin-neuraminidase (HN) genes indicated that the isolate clustered with the PPMV-1 strains recently reported from Israel within subgenotype VIb. Our findings report the emergence of PPMV-1 in Egyptian pigeons and the potential role these wild birds can play in the transmission and evolution dynamics of PPMV-1.

Acknowledgments

This work was financed by the Ministry of Higher Education (Cultural Affairs & Missions Sector), Egypt.

Conflict of Interests

The authors declare that they have no conflict of interest.

Authors’ Contribution

M.A. Rohaim., R.F. El Naggar and N. Leblanc drafted and revised the manuscript. A.M. Helal and H.A. Hussein attended to the case and performed clinical sampling and follow-up. All authors contributed to the writing of the manuscript and approved it for submission.

Figures

Figure 1
Figure 1.

Predicted 3D structures for F and HN proteins of PPMV-1 Pigeon/Egypt/VRLCU/2014 showing amino acids residues at cleavage site, fusion peptide, HR and transmembrane domain of F protein, and neutralizing epitopes and transmembrane domain of HN protein

Figure 2
Figure 2.

Phylogenetic tree  

References

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Genetic Characterization of Pigeon Paramyxovirus Type 1 in Egypt · ResearchersLinks