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Short Communication · Volume 3, Issue 7, Pages 372–376, 3 June 2015 · Open Access

Evaluation of Silver Nanoparticle Mediated Reduction of Neovascularisation (Angiogenesis) in Chicken Model

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Journal:
Advances in Animal and Veterinary Sciences
Published:
3 June 2015
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From the article

  • In the present study, silver nanoparticles were manufactured by green synthesis using Azadirachta indica (neem) leaves and its effect on process of neovascularisation was evaluated.
  • Silver nanoparticles prepared through bioconversion in the study, successfully reduced angiogenesis in embryonated chicken model and this anti-angiogenic property of Ag nanoparticles, can be explored as a potential therapeutic against pathological angiogenesis and solid tumors by targeting the vasculature.
  • Keywords | Anti-angiogenesis effect, Silver nanoparticles, Azadirachta indica, Green synthesis, Chorioallantoic membrane, Chicken model, Vasculature targeted therapy.
  • Synthesis of green silver nanoparticles using plant extracts is a very simple and cost-effective way.

Selected from the article's own text.

Abstract

Rekha Khandia1*, Ashok Munjal1, Raj Sonam Bangrey1, Reena Mehra1, Kuldeep Dhama2, Naresh Chandra Sharma1

1Department of Biochemistry and Genetics, Barkatullah University, Bhopal, Madhya Pradesh – 462026; 2Division of Pathology, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Prades-243122, India.

*Correspondence | Rekha Khandia, Barkatullah University, Bhopal, Madhya Pradesh, India; Email: rekha.morchhale@gmail.com

Abstract
Neovascularisation is a key event during the growth of solid tumor and the process is called angiogenesis. Tumor cells demands higher oxygen and their glucose requirement is also higher than normal cells, and during the growth of solid tumor new blood vessels also arise rapidly to fulfil the raised requirement of nutrients. Hence targeting the formation of new blood vessel formation leading to deprived nutrition and oxygen supply will lead to inhibited growth of tumor. In the present study, silver nanoparticles were manufactured by green synthesis using Azadirachta indica (neem) leaves and its effect on process of neovascularisation was evaluated. Bioconversion from silver nitrate solution to silver nanoparticles included addition of AgNO3 (1mM) to fresh leaf broth of Neem and the conversion was allowed to take place for 4 hrs. with continuous agitation at 80 rpm. Formation of silver (Ag) nanoparticle was visualised by change in colour from pale yellow to dark brown. Nanoparticles when subjected to developing embryonated eggs of chicken were found to reduce the number of viable blood vessels with reduction in number of vessel branch points in chorioallantoic membrane leading to the death of embryo. Silver nanoparticles prepared through bioconversion in the study, successfully reduced angiogenesis in embryonated chicken model and this anti-angiogenic property of Ag nanoparticles, can be explored as a potential therapeutic against pathological angiogenesis and solid tumors by targeting the vasculature.

Keywords | Anti-angiogenesis effect, Silver nanoparticles, Azadirachta indica, Green synthesis, Chorioallantoic membrane, Chicken model, Vasculature targeted therapy

Conflicts of Interest

The authors declare that no conflict of interest.

ACKNOWLEDGEMENTS

All the authors of the manuscript thank and acknowledge their respective Universities and Institutes.

Author’s contribution

Rekha Khandia and Ashok Munjal designed study and interpreted the results. Raj Sonam Bangrey and Reena Mehra collected review and updated article. Kuldeep Dhama and Naresh Chandra Sharma reviewed and editted the manuscript.

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