Antifungal potential of biochar produced from wood was evaluated against Fusarium oxysporum Schlecht. Methanol was used to prepare biochar extract of which various concentrations were experienced in-vitro against tested fungal strain. All the applied concentrations of methanol extract i.e. 1%, 2%, 3%, 4%, 5%, 6%, 7% and 8% effectively inhibited F. oxysporum growth. However, 7% and 8% concentrations were proved to be most efficient in retarding the test fungus growth upto 15%. Partitioning of biochar methanolic extract was done using some organic solvents such as n-hexane, chloroform, ethyl acetate and n-butanol in increasing the polarity order. These secluded fractions were further tested to evaluate their in vitro antifungal activity along with a reference fungicide (Mancozeb). Data obtained after 7 days of incubation depicts that maximum antifungal activity was observed in fungicide with 89% inhibition in the radial diameter of the test fungus. n-hexane and chloroform also effectively retarded the growth of F. oxysporum, whereas n-butanol and ethyl acetate fractions gave minimum activity . So it can be concluded that wood biochar has the potential to control F. oxysporum.