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Short Communication · Volume 3, Issue 11, Pages 613–616, 21 October 2015 · Open Access

Effect of Intrauterine Administration of Lipopolysaccharide (LPS) on the Transcriptional Profile of IL2, TNFα and IFNγ in Mice

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Journal:
Advances in Animal and Veterinary Sciences
Published:
21 October 2015
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  • Effect of Intrauterine Administration of Lipopolysaccharide (LPS) on the Transcriptional Profile of IL2, TNFα and IFNγ in Mice.
  • Abstract | The effect of intrauterine (IU) infusion of lipopolysaccharide (LPS) on some of the proinflammatory cytokine was studied in a mice model.
  • Female mice of nine week age (n=24) were divided into two equal groups; one group received LPS (E. coli O127:B8) 25μg in 100 μl PBS intrauterine while the other received PBS 100μl as vehicle.
  • Immunokinetic study was done 12 and 24hrs post-LPS treatment by sacrificing 6 mice from each group at each time and relative expression of cytokines (IL2, TNFα and IFNγ) by real time PCR in the uterine tissue.

Selected from the article's own text.

Abstract

Tumynak Loyi1, Harendra Kumar1*, Sukdeb Nandi2, Manas Kumar Patra1, Rafiqul Islam1, Narayanan Krishnaswamy1

1Division of Animal Reproduction; 2Centre of Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, 243 122, U.P. India.

*Correspondence | Harendra Kumar, Indian Veterinary Research Institute, India; Email: hkumar1960@gmail.com

Abstract
The effect of intrauterine (IU) infusion of lipopolysaccharide (LPS) on some of the proinflammatory cytokine was studied in a mice model. Female mice of nine week age (n=24) were divided into two equal groups; one group received LPS (E. coli O127:B8) 25μg in 100 μl PBS intrauterine while the other received PBS 100μl as vehicle. Immunokinetic study was done 12 and 24hrs post-LPS treatment by sacrificing 6 mice from each group at each time and relative expression of cytokines (IL2, TNFα and IFNγ) by real time PCR in the uterine tissue. Apparent up-regulation of IL2 cytokine mRNA expression at 12h (2-fold) and 24h (37-fold, P<0.05) was recorded in LPS-infused mice. Similarly, the expression of TNFα mRNA was elevated by 6 and 35-fold at 12 and 24h post infusion, respectively. The increase in the expression of IFNγ was found only at 12h (1.8-fold, P<0.05) in LPS infused mice. It is concluded that intrauterine administration of LPS in the mice elevated the expression of proinflammatory cytokines.

Keywords | Endometritis, Cytokine, Lipopolysaccharide, Mice, Intrauterine

Editor | Kuldeep Dhama, Indian Veterinary Research Institute, Uttar Pradesh, India.
Received | June 07, 2015; Revised | September 08, 2015; Accepted | September 11, 2015; Published | October 21, 2015

Citation | Loyi T, Kumar H, Nandi S, Patra MK, Islam R, Krishnaswamy N (2015). Effect of intrauterine administration of lipopolysaccharide (LPS) on the transcriptional profile of IL2, TNFα and IFNγ in mice. Adv. Anim. Vet. Sci. 3(11): 613-616.

ISSN (Online) | 2307-8316; ISSN (Print) | 2309-3331

Copyright © 2015 Loyi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

* Significance at 95% level (P<0.05)

LPS is the main pathogenic ligand of E. coli and a strong activator of innate immune response . Following infection, an immune response is initiated by cell surface Toll-like receptor-4 (TLR4), CD14 and MD2 ; , that detect pathogens and in turn trigger signaling pathways that activate molecules (transcription factors) that controls the induction of a proinflammatory immune response through activation of genes encoding cytokines (IFNγ, IL2 and TNFα) and chemokines . It is suggested that LPS can trigger TNFα release by interferon-primed macrophages and induce IFNγ production, resulting in an additive effect on the production of TNFα. In addition, a higher endometrial expression of TLR4 in clinical endometritis was reported in buffaloes . It is reported that treatment of bovine granulose cells in culture with LPS adversely affected oocyte maturation and increased IL6 transcripts and steroidogenic capacity through TLR4 pathway .

The elevated levels of TNFα and IFNγ in our study are in agreement with the reports of Nandi et al. (2010) who reported that bacterial LPS in the bloodstream induced production of inflammatory cytokines such as TNFα, IL1β and IFNγ. TNF is secreted from a variety of gestational cells upon stimulation with bacteria or lipopolysaccharides in vitro including placental fragments, amnion, and chorio-decidua . Herath et al. (2006b) also described the increased expression of TNFα in the cultured endometrial epithelial and stromal cells stimulated in vitro with LPS. However, Yang et al. (2009) did not observe a classical predominance of Th1 cytokine in the plasma of mouse of pre-term labour following intrauterine infusion of LPS.

Upregulation of IL2, IFNγ and TNFα transcripts in the uterine tissue of mice following LPS infusion in the present study indicates uterine inflammation. Intravaginal inoculation of mice with specific bovine uterine isolates of E. coli will further support the present observation. The efficacy of novel intrauterine drugs for the treatment of endometritis in the bovine may be tested using this model.

Acknowledgments

The authors are grateful to the Director, ICAR - Indian Veterinary Research Institute, Izatnagar for providing the facility to carry out the research work.

Conflict of interest

There is no conflict of interest.

AUTHORS CONTRIBUTION

The work was carried out by Tumnyak Loyi as part of her PhD thesis under the guidance of Harendra Kumar and Sukdeb Nandi. Manas Kumar Patra and Rafiqul Islam assisted in designing of experiment, sample collection, and analysis of result. Krishnaswamy Narayanan assisted in statistical analysis and preparation of the manuscript.

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Effect of Intrauterine Administration of Lipopolysaccharide (LPS) on the Transcriptional Profile of IL2, TNF&#945; and IFN&#947; in Mice · ResearchersLinks