Vinca major L. is an evergreen perennial weed of family Apocynaceae, growing naturally in hilly areas of Hazara region of Khyber Pakhtunkhwa, Pakistan. Flowers of this weed were collected during June 2021, shade dried and extracted in pure methanol for two weeks. After filtration, the extract was analyzed by GC-MS for the identification of possible bioactive compounds. α-Amyrin was the major compound (32.49%) followed by lup-20(29)-en-3-ol, acetate, (3β)- (25.72%). Moderately abundant compounds included γ-sitosterol (8.78%), β-amyrone (7.25%), cyclohexane, 1,3,5-triphenyl- (7.01%), olean-12-en-3-ol, acetate, (3β)- (5.68%), and n-hexadecanoic acid (3.18%). Some of the identified compounds have various important biological properties including anti-inflammatory, antimicrobial, anticancer, antimalarial, antioxidant, antidiabetic, antitumor, etc.