Cancer is a life-threatening disease which is emerging as a major problem in the field of medicine. Different methods are being used to eradicate cancer including chemotherapy, radiotherapy, immunotoxins, and drug therapy but these treatments cause various side effects to the cancer patients, as they not only target cancer cells but also cause harm to normal body cells. At present some live bacteria, bacterial products, and bacterial proteins are used to treat cancer. One of the amazing bacterial protein azurin can work as an anti-cancerous agent which only target cancerous cells and do not cause any harm to healthy cells and thus minimize the risk of adverse side effects. The interaction of azurin with P53 (tumor suppressor protein), Eph/ephrin receptor and Cadherin enhances the activity of azurin by inducing apoptosis in cancerous cells, disrupting signaling among cancerous cells and inhibiting the growth of cancerous cells as well as suppressing the activity of tumor cells. This review article focuses on the activity of the bacterial protein azurin as an anti-cancer agent and also the properties of azurin which makes it one of the preferable anti-cancerous agent over the other cancer treatments like chemotherapy and radiotherapy.