Study of Antiviral Potential of Cholistani Plants against New Castle Disease Virus
Study of Antiviral Potential of Cholistani Plants against New Castle Disease Virus
Mirza Imran Shahzad1, Hina Ashraf2,*, Muhammad Arshad3, Sabeeha Parveen4, Amna Aslam4, Nargis Naz4, Zahid Kamran1, Sumbul Gohar Khalid
ABSTRACT
Present study is based on evaluation of antiviral potential of methanolic extracts of eleven selected Cholistani plants against New Castle Disease Virus (NDV)-LaSota strain. All of these plants were reported for their different pharmacological activities but their antiviral potentials were not known before. The methanolic extracts were made and concentrated by rotary evaporator and finally dissolved in distilled water before taking their antiviral trials in 7-11 days old chicken embryonated eggs. The viral loads were determined through heamagglutination (HA) test. The methanolic extract of each plant was found effective against NDV but in varying order. The active extracts were further used in different concentrations and IC50 of each extract was calculated. The extract of Achyranthes aspera was found most effective with IC50 3.125μg and the extract of Oxystelma esculentum, was found least effective with IC50 60μg in the series. The current study has established the fact that Cholistani plants are rich source of anti-NDV agents and can be used in purified or in crude form.
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