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Identification of Three Cyprinidae Family Members through Cytochrome Oxidase I

Identification of Three Cyprinidae Family Members through Cytochrome Oxidase I

Shahid Sherzada1,2,4,*, Muhammad Naeem Khan4, Masroor Ellahi Babar2, Muhammad Idrees3, Abdul Wajid2, Muhammad Nauman Sharif3, Muhammad Shahzad Iqba

1Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore
2Department of Science and Technology, Virtual University of Pakistan, Lahore
3Center of Excellence in Molecular Biology, University of the Punjab, Lahore
4Department of Zoology, University of the Punjab, Lahore

*     Corresponding author: shahid.sherzada@uvas.edu.pk

 

ABSTRACT
Fishes belonging to family Cyprinidae are important for aquaculture and also for food and economy of Pakistan. Among members of Cyprinidae family, Indian major carp species (Labeo rohita, Cirrhinus mrigala, Catla catla)are very suitable fish species for culture in Pakistan. However, the identification and phylogeny of these fish species are of much interest today. DNA barcoding is used as a bio-identification tool for the organism globally. The current study was also conducted for the fast and accurate identification of Indian major carp species. The fishes used in this study were captured from four geographically isolated districts of Punjab province. Blood samples of sixty (60) fishes were taken carefully from caudal veins of each captured fish species. Genomic DNA was extracted from blood and short segment COI gene (680bp) was amplified by PCR. The sequences were evaluated by using bioinformatics tools. The average K2P conspecific, congenric and confamilial genetic distances were 0.22%, 6.71% and 12.33%, respectively. Findings of the present study revealed that DNA barcoding using COI gene is an efficient molecular technique for the exact identification of typical fish species.

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Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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