Effect of Iron Chloride on Peroxidase Activity in Kidney and Liver of Labeo rohita
Rimsha Nawaz1, Muhammad Javed1, Sidra Abbas1* and Safina Kousar2
Present research work was conducted to evaluate the effects of iron chloride on peroxidase enzyme activity in the fish, Labeo rohita. For this purpose, four groups of Labeo rohita (one-year old) were exposed to different treatments as 96-h LC50, 2/3rd, 1/4th and 1/5th of LC50 concentrations of iron chloride for 30 days in the glass aquaria with three replications for each treatment. After 30-day exposure of iron chloride, the fish organs viz. kidney and liver were isolated and the peroxidase enzyme activity was analyzed. Activity of peroxidase was assessed by measuring the conversion of guaiacol to tetraguaiacol, spectrophotometrically, at a wavelength of 470 nm. The peroxidase activity was measured in the selected organs exposed to various sub-lethal concentrations of iron chloride. The results revealed that peroxidase activity was increased significantly in both kidney and liver after exposure to iron chloride in all the treatments as compared to the control group. It was found that enzyme peroxidase had activity of 0.364±0.004 and 0.588±0.004 UmL-1 in the metal stressed fish kidney and liver, respectively while in control fish, the same for both the organs were observed as 0.085±0.002 and 0.112±0.002 UmL-1, respectively.