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A Prl/RsaI Polymorphism in Exon 3 and 4 of Prolactin Gene in Dairy Cattle

A Prl/RsaI Polymorphism in Exon 3 and 4 of Prolactin Gene in Dairy Cattle

Memis Ozdemir

Department of Animal Science, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey

*      Corresponding author: ozdemirm@atauni.edu.tr 

 

ABSTRACT
Prolactin is a quantitative trait locus and is a potential genetic marker that can be used in the improvement of production traits in dairy cattle, because prolactin has an important regulatory role in the development of the mammary glands, the secretion of milk and the expression of milk protein genes. It has been seen many studies about Prl/RsaI polymorphism found in the exon 3 or exon 4 region of bovine prolactin gene in the literature. The aim of this study was to determine whether the DNA sequence of the Prl gene exon 3 or exon 4 in cattle breeds has the RsaI polymorphic digestion site. As a result of the study, it has been seen that the Prl/RsaI specificpolymorphic site is on exon 4 in Bos taurus cattle breeds, but have been not detected the RsaI restriction enzyme digestion site in Prl gene exon 3 region reported by many literature and, these results also have been approved by blasted at the NCBI Genbank.

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Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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