cDNA Cloning and Expression of Cyclophilin A (LvCypA) in White Leg Shrimp, Litopenaeus vannamei
Faiz Muhammad,1, 2,*, Zhang Zhi-Feng,1, Shao Ming-Yu1 and Muhammad Shafi3
Sequences of nucleic acid and deduced amino acid from the white leg shrimp L. vannamei Cyclophilin A. The polyadenylation signal sequence is underlined, the asterisk shows the stop codon. The red box is showing the Cyp- type peptidyl-prolyl cis- transisomerase signature. The black boxes are showing the glycosylation sites.
ClustalX aligment and comaprison of the amino acid sequences deduced from Lv-CypA cDNA with some similar protein sequences of cyclophilin A. The others simialr proteins are predicted and obtained from GenBank as follows: Penaeus monodon (ABV90639.1), Eriocheir sinensis (AEC48729.1), Chlamys farreri (AAR11779.1), Artemia franciscan (ABN13586.1), Bombyx mori (NP_001037301.1), Ictalurus punctatus (NP_001187167.1), Beauveria bassiana (AAN39296.1), Danio rerio (CAX13413.1), Homo sapiens (AAU13906.1). Residuces in black background indicate higher level of amino acid similarity. The four red boxes are showing the beta strands while the blue box is showing the existance of cis-trans isomerase.
A phylogenetic tree constructed with the neighbor- joining method. The tree is based on an alignment corresponding to full length amino acid sequences, using ClustalX and megAlign. The numbers shown at the branches denote bootstrap majority consensus values of 1000 replicates. The GenBank accession numbers are same as given in Figure 2.
Expression of LvCypA in different tissues of shrimp. The abundance of LvCypA mRNA was normalized with β-actin. Vertical bars representing the mean value error. HP, hepatopancreas; M, muscle; G, gill; LO, lymphoid organ.