Screening of Okra Cultivars for Resistance against Helicoverpa armigera
Screening of Okra Cultivars for Resistance against Helicoverpa armigera
Ajmal Khan Kassi1,*, Humayun Javed1 and Tariq Mukhtar2
1Department of Entomology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi
2Department of Plant Pathology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi
Fruit borer, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is one of the most economically important and widespread pests of okra and causes worldwide yield losses. The pest is mainly controlled by chemicals which cause deleterious effects to human, livestock and environment. To dispense with the detrimental consequences of chemicals, use of resistant cultivars can prove one of the effective and reliable alternative approaches for the management of this pest. As the information regarding resistant cultivars is lacking, therefore, the objective of the present study was to identify resistant sources from among the available commercial okra cultivars. Okra cultivars showed significant variations from comparative susceptibility to comparative resistance. On the basis of fruit and shoot infestation caused by H. armigera, Manchali, RK-516, OH-152 and Tarnab Nargis were found comparatively susceptible, Rama Krishna, NS-810, Baharti Kaspori, Lady Finger-1 and Lady Finger-2 showed intermediate resistant reaction while KT-458, Bhindi Punjab Selection, Arka Anamika and Bhindi Sabazpari proved to be comparatively resistant. Maximum fruit and shoot infestations were recorded on comparatively susceptible cultivars while the infestations were the minimum on comparatively resistant cultivars. As comparatively resistant cultivars viz. KT-458, Bhindi Punjab Selection, Arka Anamika and Bhindi Sabazpari suffered less damage and therefore, are recommended for cultivation to avoid damage by Helicoverpa armigera.
Received 11 August 2017
Revised 16 September 2017
Accepted 27 September 2017
Available online 27 December 2017
AKK, HJ and TM designed the study, executed experimental work and analyzed the data. HJ supervised the work. TM helped in preparation of the manuscript.
American boll worm, Resistance, Susceptibility, Fruit infestation, Shoot infestation.
* Corresponding author: email@example.com
0030-9923/2018/0001-0091 $ 9.00/0
Copyright 2018 Zoological Society of Pakistan
Okra (Abelmoschus esculentus) is one of the important summer vegetables grown in many countries of the world (). Being a rich source of many nutrients, it is used in curries and taken as stewed and soups. It contains 25 calories, 2 g dietary fiber, 1.5 g protein, 5.8 g carbohydrates, 460 IU vitamin A, 13 mg vitamin C, 36.5 μg folic acid, 50 mg calcium, 0.4 mg iron, 25 mg potassium and 46 mg magnesium per half cup of okra. In Pakistan, it is widely cultivated (; ) and its area is increasing every year. Pakistan contributes 2% share in the total production of the world. The area under cultivation in Pakistan is about 14.5 thousand hectares with an annual production of 109.3 thousand tons ().
The successful production of okra is affected by many biotic factors including fungi (; ), viruses (, , , ), nematodes (; ; , ,), bacteria (; ,) and particularly the insect pests. The fruit borer, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is one of the most economically important and widespread pests of okra and causes worldwide infestation to okra and other crops and vegetables (; ;; ). The females have strong preference for oviposition on okra plants (). The larval stage directly causes damage to flowers, buds and fruits and incurs heavy yield losses in crops. Heavy yield losses have been reported by many researchers on different crops and vegetables by this pest (; ; ; ). Annual losses of about US$ 2 billion have been reported in semiarid tropics, even though US$ 500 million worth pesticides are applied to control the pest ().
The borer is mainly controlled by using synthetic pesticides in Pakistan. The use of pesticides is not often without associated harmful effects and the most commonly confronted are resurgence of secondary pests, pesticide pollution, elimination of beneficial insect fauna, pest resistance to specific insecticides and various human health problems. To eliminate these side effects, alternative approaches must be adopted to reduce dependency on chemical pesticides. One of the effective and reliable approaches for controlling the pest is the use of resistant cultivars. The resistant cultivars can minimize yield losses and can also be used as a constituent of integrated pest management programs along with other control strategies (; ). As the information regarding resistant cultivars is lacking, therefore, the objective of present research was to identify resistant sources among the available commercial okra cultivars.
Tarnab Nargis, RK-516, OH-152, Manchali
Lady Finger-1, NS-810, Baharti Kaspori, Lady Finger-2, Rama Krishna
KT-458, Bindi Sabazpari, Arka Anamika, Bhindi Punjab Selection
Materials and methods
The plant material tested for resistance to H. armigera consisted of thirteen cultivars collected from National Agricultural Research Centre, Islamabad and authorized seed dealers of Rawalpindi. These included Baharti Kaspori, Rama Krishna, Tarnab Nargis, Bhindi Sabazpari, KT-458, Lady Finger-1, Manchali, OH-152, NS-810, Arka Anamika, Bhindi Punjab Selection, Lady Finger-2 and RK-516. The experiment was conducted at the University Research Farm Koont of Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan located at 32°56’0” North and 72°52’ 0” East. Randomized Complete Block Design was used to evaluate okra cultivars. Plot measuring 40′×10′ was used for each cultivar with three replications. The overnight-presoaked seeds were sown in each plot with plant to plant and row to row distances of 60 and 75 cm, respectively. Irrigation and all other agronomic practices were applied as per requirement.
Fruit and shoot infestations were recorded on ten randomly selected plants from each experimental unit. From each of the ten specified okra plants, healthy and damaged fruits and shoots were observed and shoot and fruit infestation were recorded as described by and . The cultivars were categorized as comparatively susceptible (shoot infestation >14% and fruit infestation >10%), intermediate resistant (shoot infestation <14% and fruit infestation <8%) and comparatively resistant (shoot infestation <10% and fruit infestation <5%). All the data regarding fruit and shoot infestation percentages were subjected to statistical analysis by Costat package and their means were compared using DMR Test at 5% level of probability ().
Results and Discussion
On the bases of fruit and shoot infestations caused by H. armigera larvae, Manchali, RK-516, OH-152 and Tarnab Nargis were found comparatively susceptible, Rama Krishna, NS-810, Baharti Kaspori, Lady Finger-1 and Lady Finger-2 showed intermediate resistant reaction while KT-458, Bhindi Punjab Selection, Arka Anamika and Bhindi Sabazpari appeared as comparatively resistant (). Fruit and shoot infestations on each variety have been shown in and . It is clear from the and that maximum fruit and shoot infestations were recorded on comparatively susceptible cultivars while the infestations were the minimum on comparatively resistant cultivars. Many researchers have evaluated different okra varieties under varying climatic conditions in different okra producing countries. In the present studies okra cultivars showed variable infestations of H. armigera on fruit and shoot. The minimum fruit infestation was observed on Arka Anamika (3.3%) fallowed by Bhindi Punjab Selection (4.1%) and Bhindi Sabazpari (4.8%). The findings are to some extent similar to those observed by , and who have reported low fruit infestation on Sabzpari. The cultivar Rama Krishna with 5.5% fruit infestation showed intermediate resistance against H. armigera. The findings are not similar to the results observed by on Rama Krishna with 9.4% fruit infestation. This variation in infestation might be due to differences in temperature, humidity and rainfall. Arka Anamika and Bhindi Punjab Selection were found comparatively resistant cultivars with 3.3 and 4.1% fruit infestations, respectively. The findings are in accordance with those of who also found that Punjab Selection was moderately resistant against H. armigera.
Earlier many researchers have studied resistance in many okra cultivars and reported role of different physico-morphic characters in imparting resistance against the insect pests (, ). Similarly, density of hairs on crown leaves () and leaf hairiness () had a significant effect on the selection of oviposition sites by Earias vittella (Fab.). Conversely, several workers have reported that okra cultivars showing greater fruit infestation had more hair density and soft skin (). had also reported that fruit damage by E. vittella larvae was higher in open pollinated varieties than hybrids.
Okra cultivars showed significant variations in fruit and shoot infestations from comparative susceptibility to comparative resistance. Four cultivars viz. KT-458, Bhindi Punjab Selection, Arka Anamika and Bhindi Sabazpari appeared as comparatively resistant and suffered less damage and hence are recommended for cultivation to avoid damage by Helicoverpa armigera. The resistant cultivars can minimize yield losses and can also be used as a component in integrated pest management programs along with other control strategies.
Statement of conflict of interest
The authors declare that there is no conflict of interests regarding the publication of this article.
Afzal, M., Mukhtar, K.M., Tahir, H.M., Ibrar-ul-haq, Babar, M.H. and Sherawat, S.M., 2015. Screening of different okra genotypes against fruit borer (Earias spp.) (Lepidoptera: Noctuidae) on okra crop. Pakistan J. Zool., 47: 1631-1635.
Ashfaq, M., Khan, M.A., Mukhtar, T. and Sahi, S.T., 2014b. Role of mineral metabolism and some physiological factors in resistance against urdbean leaf crinkle virus in blackgram genotypes. Int. J. Agric. Biol., 16: 189-194.
Ashfaq, M., Saleem, A., Waqas, M. and Mukhtar, T., 2017. Natural occurrence and host range studies of Cucumber Mosaic Virus (CMV) infecting ornamental species in Rawalpindi-Islamabad area of Pakistan. Philipp. Agric. Scient., 100: 55-61.
Aziz, M.A., Hasan, M., Ali, A. and Iqbal, A., 2012. Comparative efficacy of different strategies for management of spotted bollworms, Earias spp. on okra, Abelmoschus esculentus (L.) Moench. Pakistan J. Zool., 44: 1203-1208.
Hussain, M.A., Mukhtar, T. and Kayani, M.Z., 2014. Characterization of susceptibility and resistance responses to root-knot nematode (Meloidogyne incognita) infection in okra germplasm. Pak. J. agric. Sci., 51: 319-324.
Iqbal, U. and Mukhtar, T., 2014. Morphological and pathogenic variability among Macrophomina phaseolina isolates associated with mungbean (Vigna radiata L.) Wilczek from Pakistan. Scient. World J., 2014: Article ID 950175.
Iqbal, U., Mukhtar, T. and Iqbal, S.M., 2014. In vitro and in vivo evaluation of antifungal activities of some antagonistic plants against charcoal rot causing fungus, Macrophomina phaseolina. Pak. J. agric. Sci., 51:689-694.
Jallow, M.F.A., Cunningham, J.P. and Zalucki, M.P., 2004. Intra-specific variation for host plant use in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae): implications for management. Crop Prot., 23: 955-964.
Jallow, M.F.A., Maatsumura, M. and Suzuki, Y., 2001. Oviposition preference and reproductive performance of Japanese Helicoverpa armigera (Huebner) (Lepidoptera: Noctuidae). Appl. Ent. Zool., 36: 419-426.
Javed, H., Mukhtar, T., Javed, K. and Mohsin, A., 2017. Management of eggplant shoot and fruit borer (Leucinodes orbonalis Guenee) by integrating different non-chemical approaches. Pak. J. agric. Sci., 54: 65-70.
Khan, A.R., Javed, N., Sahi, S.T., Mukhtar, T., Khan, S.A. and Ashraf, W., 2017. Glomus mosseae (Gerd & Trappe) and neemex reduce invasion and development of Meloidogyne incognita. Pakistan J. Zool., 49: 841-847.
Mironidis, G.K. and Savopoulou-Soultani, M., 2008. Development, survivorship and reproduction of Helicoverpa armigera (Lepidoptera: Noctuidae) under constant and alternating temperatures. Environ. Ent., 37: 16-28.
Rahoo, A.M., Mukhtar, T., Gowen, S.R., Rahoo, R.K. and Abro, S.I., 2017. Reproductive potential and host searching ability of entomopathogenic nematode, Steinernema feltiae. Pakistan J. Zool., 49: 229-234.
Shahbaz, M.U., Mukhtar, T., Haque, M.I. and Begum, N., 2015. Biochemical and serological characterization of Ralstonia solanacearum associated with chilli seeds from Pakistan. Int. J. Agric. Biol., 17: 31-40.
Shahzaman, S., Inam-ul-Haq, M., Mukhtar, T. and Naeem, M., 2015. Isolation, identification of antagonistic rhizobacterial strains obtained from chickpea (Cicer arietinum L.) field and their in-vitro evaluation against fungal root pathogens. Pak. J. Bot., 47: 1553-1558.
Tariq-Khan, M., Munir, A., Mukhtar, T., Hallmann, J. and Heuer, H., 2017. Distribution of root-knot nematode species and their virulence on vegetables in northern temperate agro-ecosystems of the Pakistani-administered territories of Azad Jammu and Kashmir. J. Pl. Dis. Prot., 124: 201-212.
Yu, F.L., Wu, G., Liu, T.J., Zhai, B.P. and Chen, F.J., 2008. Effects of irrigation on the performance of cotton bollworm, Helicoverpa armigera (Hübner) during different pupal stages. Int. J. Pest Manage., 54: 137-142.