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Proteomic Analysis by Shotgun LC-MS/MS of Cyst Wall Protein Components From the Protozoan Parasite Cryptocaryon irritans, the Causative Agent of White Spot Disease in Marine Fish

Proteomic Analysis by Shotgun LC-MS/MS of Cyst Wall Protein Components From the Protozoan Parasite Cryptocaryon irritans, the Causative Agent of White Spot Disease in Marine Fish

Honghua Wang1,2, Summia Perveen1,2, Shucheng Shao1,2, Kun Wang1,2 and Fei Yin1,2,*

1Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Collaborative Innovation Centre for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, People’s Republic of China
2School of Marine Sciences, Ningbo University, 169 South Qixing Road, Ningbo 315832, People’s Republic of China

*      Corresponding author: feige895@gmail.com

 

Fig. 1.

Cyst wall of Cryptocaryon irritans viewed by an optical microscope (A, B), SEM (C), and TEM (D). A, a holistic view of empty cyst walls; B, partial view of empty cyst walls; C, cyst wall stripped off in SEM sample preparation, showing the multi-layer structure of the thick cyst wall; D, sectional view of the cyst wall. ECWs represent empty cyst wall. Arrows indicate the inner surface of cyst wall, and Arrowheads indicate the outer surface of the cyst wall. Scale bars = 500 μm (A), 10 μm (B), 10 μm (C), and 1 μm (D).

Fig. 2.

Distributions of molecular mass (MW) for cyst wall of Cryptocaryon irritans by the shotgun LC-MS/MS approach.

Fig. 3.

Distributions of isoelectric point (pI) for cyst wall of Cryptocaryon irritans by the shotgun LC-MS/MS approach.

Fig. 4.

GO categories of Cryptocaryon irritans cyst wall proteins. These terms were summarized into 2167 sub-categories under 3 GO terms. Bar charts represented the top 20 sub-categories of the biological process (BP) category, cellular component (CC) category, and molecular function (MF) category, respectively. Grey bars showed the number of proteins, the black bars indicated the percentage of proteins.

Fig. 5.

KEGG categories of Cryptocaryon irritans cyst wall proteins. A total of 2,190 proteins were annotated by KEGG and classified into 6 categories with 47 subclasses. The upper cases represent the title of 6 categories. A, metabolism; B, genetic information processing; C, environmental information processing; D, cellular processes; E, organismal systems; F, human diseases.

Fig. 4

Proteomic Analysis by Shotgun LC-MS/MS of Cyst Wall Protein Components From the Protozoan Parasite Cryptocaryon irritans, the Causative Agent of White Spot Disease in Marine Fish

Pakistan Journal of Zoology

June

Vol. 52, Iss. 3, Pages 825-1224

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