Research Article

Phenology and Maize Crop Stand in Response to Mulching and Nitrogen Management

Muhammad Ibrahim and Ahmad Khan*

Department of Agronomy, The University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.

Received | April 27, 2017; Accepted | August 10, 2017; Published | August 31, 2017

*Correspondence | Ahmad Khan, Department of Agronomy, The University of Agriculture, Peshawar, Pakistan; Email: [email protected]

Citation | Ibrahim, M. and A. Khan. 2017. Phenology and maize crop stand in response to mulching and nitrogen management. Sarhad Journal of Agriculture, 33(3): 426-434.

DOI | http://dx.doi.org/10.17582/journal.sja/2017/33.3.426.434

Keywords | Mulching, Manure, Phenology, Crop stand, Nutrients turn over

Introduction

Maize (Zea mays L.) is the third most important crop of Pakistan after wheat and rice and is the second in importance in Khyber Pakhtunkhwa after wheat (Asif et al., 2009). It is a multipurpose crop that provides food for human, feed for animals especially poultry and livestock and raw material for the industries. Average maize yield is low (1943 kgha-1) in Khyber Pakhtunkhwa as compared with the national average yield (4155 kgha-1) which itself is lower than major maize producing countries like US, China, Brazil, European union and India. The reasons of low maize productivity in Khyber Pakhtunkhwa and in the country is the low availability of water, weeds infestation and unavailability of sufficient fertilizer and their management in maize growing areas.

Crop stand is directly related to the seed quality (Khan and Khalil, 2005), however the optimum soil nutrients availability (Rahman et al., 2013) and seed bed condition (Zaman and Khan, 2016) also affect the crop stand. Non uniform crop stand will affect the phenological occurrences, which will affect the crop

fertilization and hence will increase the barren plants (Khan et al., 2015). Mulching is one of the management practices for increasing water conservation and efficiently controlling weeds infestation in field crops (Ram et al., 2013). Organic mulches perform additional functions like increasing soil organic matter content, enhance biological activity (Zhao et al., 2012), improve soil structure and increase plant nutrients after decomposition (Depar et al., 2016). Mulch have positives effects both on soil and plants i.e. provides a better soil environment, moderates soil temperature (Anikwe et al ., 2007),increases soil porosity (Shaxson and Barber, 2016), optimize water infiltration rate during intensive rain (Bu et al., 2013), control runoff and erosion, suppress weed growth, and conserve the soil moisture content (Depar et al., 2016).

Nitrogen is considered a key element affecting crop phenology (Khan et al., 2008) and is the basic building material of protein and nucleic acid and its reduction causes negative effects on crop growth (Ge et al., 2013). It is extremely linked with dark green colour of stem and leaves (Perini et al., 2016), vigorous growth (Pang et al., 2014), branching and leaf expansion (Saleem et al., 2009). Commercial form of N is readily available to the plants and had increased the yield of maize crop (Liu et al., 2016). However, it is expensive for small farmers (Kumar et al., 2014), and cause environmental pollution via leaching into ground water. Therefore, farmyard manure (FYM) can be considered as alternative source of N, which markedly increased plant’s productivity by providing essential plant nutrients (Kumar et al., 2015), especially the N, improving soil physical properties (Anand et al., 2014) and water holding capacity of the soil. The addition of FYM to the soil is used since centuries particularly in the developing countries. However, its nutrients concentration is far lower than commercially available nitrogenous fertilizers (Kannan et al., 2013). Due to low N content, it is applied in the bulk quantity (Storkey et al., 2016), which limits its application for farming purpose due to high transportation cost. Thus, the integration of FYM with commercial fertilization is the possible solution for the use of FYM in farming practices.

The mulching techniques had greater role for moisture conservation whereas N management had proven to improve soil fertility and crop production. However, research gap still exist to quantify their effects on maize crop stand and phenological occurrence. Therefore, the present field experiments were conducted to find out the effect of N management treatments under both plastic and residue mulching on crop stand and phenological occurrence of maize in agro-climatic conditions of Peshawar valley.

Materials and Methods

Experimental site

To quantify the effects of mulching and nitrogen management on maize crop stand, and phenological occurrences, field experiments were conducted at Agronomy Research Farm, The University of Agriculture Peshawar-Pakistan during 2013 and repeated in 2014. The experimental site has semiarid climatic condition, with average temperature of 18-33 °C (2013) and 15-34°C (2014), with mean monthly rainfall of ~ 60 mm during the experimental periods (Figure 1). The soil was deficient in organic matter, total N, and phosphorus, whereas the potash content in the soil was in optimum range (Table 1). The soil of the experimental site was silt loam, piedmont alluvium Ustochrepth based on USDA classification (Anonymous, 2007).

Table 1: Physico-chemical properties of soil samples of the experimental site and added farmyard manure as a source of nitrogen.

Physico-chemical properties	Units	Soil	FYM	Canola residue
Organic matter	%	0.67	16.8	42.6
Total nitrogen	%	0.042	0.87	1.17
Mineral nitrogen	mg kg-1	0.835	0.13	0.31
pH	-	8.23	6.8	6.5
EC	dS m-1	0.45	5.41	3.3
EDTA extractable P	g kg-1	0.11	3.62	3.81
EDTA extractable K	g kg-1	0.13	4.91	2.14
Sand	%	20.17	-	-
Silt	%	69.62	-	-
Clay	%	10.21	-	-
Textural Class		Silt loam	-	-

 

Materials and treatments

Farmyard manure (FYM) and canola residue (remaining part of the canola excluding seed i.e. stalk, leaves, pods) were obtained from the Dairy Farm, and Agronomy Research Farm, The University of Agriculture Peshawar, respectively and were analysed for physico-chemical properties (Table 1). The experimental treatments included mulching practices (no-mulch, canola residue mulch, transparent plastic mulch, and blue colour plastic mulch) and 250 kg N ha-1 sources (i.e. control, 100, 70, 50, 0% from FYM with the remaining from urea). The experiment was laid out in RCB design with split plot arrangements having four replications. Different mulching practices were allotted to main plots while different nitrogen management treatments to sub plots. The FYM application were made 30 days before sowing in the respective plots, whereas urea was applied in split application half at sowing and half after first irrigation. Sub plot size was 3.6 x 6 m having 8 rows 75 cm apart and 3.6 m long. Maize hybrid Pioneer 3025 was planted in ridges. The planting was made manually with plant to plant distance of 20 cm, using two seeds hill-1, and was thinned to single seedling after 15 days of emergence. Basal dose of P and K was applied to the crop at the rate of 120 kg ha-1 each. No herbicides were used during the experiment, however to control borer attack, Furadan (Carbofuran 3%) at the rate of 20 kg ha-1 was applied in the early stages of maize growth. A total of six irrigations (as flood irrigation) were made during critical growth stages (emergence, 3 leaves stages, knee height, tasseling/silking, grain development, and grain filling) both in 2013 and 2014.

Observations and measurements

Phenological stages were measured in term of days to emergence, tasseling, silking and physiological maturity. The days’ difference between sowing and the stages occurrence was calculated, and was considered as days to a particular stage. Emergence was considered when 80% plants were emerged. Data on tasseling and silking was taken, when 50% plants developed tassel and silk. However the yellowing of cobs in around 90% plants was taken as physiological maturity stage. Crop stand was considered in term of emergence m-2 (recorded in third week of July each year) and plants at harvest (after physiological maturity). After complete emergence and/or at physiological maturity stages, seedling/plants in two central rows were counted and were converted into m-2 or ha-1 accordingly.

Statistical analysis

General linear mixed model was used for statistical analysis of the data. The mulching and nitrogen management were considered as main and fixed factors, year as repetitive and also fixed factor whereas the replication was considered as random factor. The analysis was carried out using statistical software statistix 8.1 version (Analytical-Software, 2005), and the means were separated using LSD post hoc multiple comparison test at p < 0.05 (Jan et al., 2009).

Results and Discussion

Year effects on maize phenological stages

Phenological stages data (Table 2) showed that days to emergence was not affected by year, whereas days to tasseling, silking, and physiological maturity was significantly delayed in 2nd year as compared to 1st year. Over the years, the greater mineralization of added FYM (Rahman et al., 2013) might have increased the mineral N contents of the soil and thus might have increased the vegetative growth of the crop and thereby delayed phenological stages (Khan et al., 2008).

Mulch effects on maize phenological stages

Control plots took more days to emergence as compared to mulched plots. Specifically, days to emergence was enhanced by two days in colour plastic mulched plots over no mulch practices (Table 2). Maize emergence depends on internal food sources of the seed (Saharan et al., 2016), moisture availability (Waterworth et al., 2015), and soil optimum temperature (Imran et al., 2013). Plastic mulch had increased the water retention capacity of the soil (Fan et al., 2017) by reducing evaporation losses (Li et al., 2013b). Similarly, mulching plots have more optimum and uniform temperature (Li et al., 2013a) over no mulch plots. The greater water storage capability and improved available water, and uniform and optimum temperature might have resulted in enhanced emergence in colour plastic mulch plot over no mulch plots.

The tasseling in maize was delayed by about two days

Table 2: Phenological stages (days to emergence, tasseling, silking and physiological maturity) of maize crop as affected by mulching practices and N sources over years.

Treatments	Days to
	Emergence	Tasseling	Silking	Maturity
Years
2013	7	47	51	98
2014	6	48	53	101
Significance	NS	*	*	**
Mulching practices (M)
No-mulch	8 a	46 c	50 c	96 b
Canola residue	7 b	48 a	54 a	100 a
Transparent plastic	6 c	47 b	51 b	100 a
Colour plastic	6 c	48 a	53 a	101 a
LSD0.05	0.3	1.0	1.1	2.0
Nitrogen management (NM)†
Control (no-fertilization)	6 a	45 c	49 c	94 e
100% FYM	7 a	46 b	52 b	98 c
70% FYM	6 a	48 a	53 a	96 d
50% FYM	6 a	48 a	53 a	101 b
0% FYM	6 a	48 a	53 a	106 a
LSD0.05	NS	1.0	1.1	1.8
Interactions P-values
Y x M	0.0870	0.1350	0.0326	0.8410
Y x NM	0.9596	0.1379	0.0242	0.0008
M x NM	0.7185	0.0070	0.0395	0.0208
Y x M x NM	0.5064	0.0866	0.1233	0.0875

†: The remaining percentages were supplied from urea to provide 250 kg N ha-1. Means within each category followed by different letter (s) are significantly different using LSD test at p≤0.05.

in residue/colour plastic mulched plots (48.3 days) compared to no mulch treatments (45.9 days) over two years average data (Table 2). Among, the mulching treatment, transparent plastic mulched plots took significantly less days to tasseling than residue and colour plastic mulched treatments. From Table 2, it is evident that silking was delayed by about three days in residue mulched plots (53.5 days) over no mulch treatments (50.2 days). Likewise, the transparent plastic mulched plots took significantly lesser days to silking (51.3) than both colour plastic (52.7 days) and residue mulch plots (53.5 days). Physiological maturity (Table 2) was delayed by about five days in colour mulched plots over no mulch treatments. However, there were no significant differences among the mulch treatments for physiological maturity. Phenology of maize depends on the plant performance, vegetative growth (Zhang et al., 2014), nutrients availability (Zhang et al., 2013), moisture availability, soil conditions (Singh et al., 2015) and crop stand (Khan et al., 2008). The improved availability of nutrients (Bowles et al., 2014), moisture contents and regulated soil temperature (Gosh et al., 2006) might have increased the vegetative growth of plant in residue mulched plots (Wang et al., 2012) and thus might have delayed phenology.

Nitrogen management effects on maize phenological stages

Control plots took less days to tasseling compared to others treatments (Table 2). Increasing the organic proportion of nitrogen from FYM, days to tassel formation were enhanced from 48.9 days (50% N from FYM) to 46.4 days (100 % N from FYM). No significant differences were observed in days to tassel formation in plots having 50% N each from urea/FYM (48.9) and 100% N from urea (48.3). Days to silking (Table 2) were earlier in control plots as compared to the rest of N treatments. Maximum days to silking (53.3 days) were recorded in plots having 50% N each from urea/FYM. No significant differences were observed in plots having 100%N derived from FYM (51.9 days), 70% N from FYM (52.5 days) and 100% N from urea (52.5 days) averaged over years. It was observed that control plots (Table 2) took less days to physiological maturity as compared to others treatments. Plots received 100% nitrogen from urea delayed maturity by twelve days over control plots. The delayed phenological stages (tasseling, silking, and maturity) in fertilized plot are associated with greater nutrients availability (Bowles et al., 2014) as a result of mineral N (urea) or N use efficiency or enhanced mineralization (Bowles et al., 2014). The application of FYM in conjunction with urea might have increased the SOM (Zhao et al., 2012), which could be another possible reasons for greater nutrients availability (Li et al., 2014) and hence delayed phenology.

Interactive response of mulching and nitrogen management for phenological stages

Regarding M × NM interaction (Figure 2), decreasing the proportion of N derived from FYM from 100% (FYM) to 0% (sole urea), the days to tasseling decreased in no-mulch plots, whereas in plastic mulched plot, days to tasseling increased. However, in case of residue mulched plots, days to tasseling delayed when the proportion of N derived from FYM decreased from 100 to 50%. The M × NM interaction (Figure 3) for days to silking indicated that days to silking remained unchanged in no-mulch plots with decreasing N proportion derived from FYM. Similarly, the decreasing proportion of N derived from FYM from 100% (FYM) to 0% (sole urea), the days to silking delayed in plastic and residue mulched plots. However, a sharp decrease was observed in residue plots as compared to plastic mulched plots. The M × NM interaction for physiological maturity (Figure 4) showed that in plastic and residue mulched plots, days to physiological maturity was more strongly affected by decreasing N proportion from FYM as compared to no-mulch plots.

Crop stand of maize

Neither emergence m-2 nor plant density was significantly affected by season. However mulching had significant effects on both emergence m-2 (Figure 5) and plant density (Figure 6) whereas nitrogen management treatments have significant effects on plant density (Figure 6).

Plots having no mulch practices had less emergence m-2 over mulch practices plots (Figure 5). Emergence m-2 was increased by 5% (residue mulch), 10% (transparent plastic mulch) and 9% (colour plastic mulch) over no mulch practice plots (Figure 5). Germination (Vashisth and Nagarajan, 2010), soil temperature, moisture content (Materechera et al., 2007), and soil physical characters (Moraru et al., 2013) affect the maize emergence. Plastic mulched plots had more germination percentage (Moraru et al., 2013), and moisture accumulation (Rodriguez, 1997) which allow the easy emergence of plumule and thus might have increased the emergence m-2. Similarly, mulching possibly protect the soil aggregates from direct rainfall impact (Rodriguez, 1997), reducing the soil hardpan and thus might have improved emergence. Greater emergence in mulch plots (87%) over no-mulch (64%) were reported by Materechera et al. (2007).

 

The emergence was minimum in control plot (22), and maximum (23) in plots having 50% N from both sources of N i.e. urea and FYM (Figure 6). Plots having 50% N derived from urea and 50% from FYM had higher emergence m-2 than control plots. The greater emergence m-2 is related to better moisture regime in the root zone in plots having FYM (Chiromaet al., 2006). The reduced evaporation demand and soften soil by manure application (Jama and Ottman, 1993) might have fulfilled the crop water demands, and thus had improved emergence m-2. Similarly, the plant population (Figure 6) was significantly higher in plots having 50% N derived from each FYM/urea (65754), followed by plots receiving sole urea (65352) as compared to the minimum plant population observed in control plots (64434). Plant population depends on soil properties (Chen et al., 2011), row spacing (Soratto et al., 2012), environmental condition and fertilizer applications (Chen et al., 2011). The improved plant nutrients availability in the fertilized plots (Ali et al., 2015) might have resulted in maintaining the optimum plant population. However, in case of control plots, the lack of nutrients might have resulted in more plants mortality (McDowell, 2011), which had decreased the plant population. The integrated application of nutrients have promoted the soil physical and biological properties (Tadesse et al., 2013). It has improved water holding capacity (Lawal and Girei, 2013), and balanced nutrient provision (Tadesse et al., 2013). All these improved roots development for nutrients uptake and thus might have decreased the plant mortality rate, and consequently, improved the plant population.

Conclusions and Recommendations

It was concluded from the experiments that phenology was delayed in 2014 compared to 2013, however the crop stand was not different in both years. Plastic mulching had enhanced emergence, whereas no-mulching have early tasseling, silking and physiological maturity. Mulching had no effects on plant density, but increased emergence over no-mulching. The control plots have earlier tasselling, silking, and maturity but decreased both emergence m-2 and plant density. Delayed phenological stages and reduced crop stand in all mulching practices were noted with increase in N proportion from urea up to 100%. The application of 50% N from FYM had 2% more plant at harvest than control plot. Thus, an optimum combination of using 50% N from farmyard manure and 50% from urea was more suitable for appropriate phenological stages and improved crop stand.

Authors Contribution

MI coonducted field experiemtns, worked on date collection, tabulation and compilation with draft preparation of the manuscript. AK supervised him during collection, analysis, preparation of draft and made corrections before and during publication process.

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