The present study aimed to explore firstly the changes in physiological parameters and oocyte quality after ovarian transplantation upon dietary supplementation of 1.0 and 2.0% of Nigella sativa (N. sativa) oil to female mice and secondly to explore if dietary N. sativa is effective in alleviation of hypothermia and hyperglycemia due to general anesthesia. Seventy-five albino female mice (21.74 ± 0.19) were distributed into three groups; control (G1; N=25; not receive N. sativa oil) and two N. sativa oil groups supplemented with either 1.0% (G2; N=25) or 2.0% (G3; N=25) for 30 days. Changes of body temperature, heart rate, blood parameters (RBCs, WBCs, hematocrit, hemoglobin, glucose and total protein), oocyte quality after ovarian transplantation (cumlus enclosed, brilliant cresyl blue stain and diameter) and reproductive performances (litter size and weight) were determined and recorded. In addition, values of body temperature and blood glucose were determined after general anesthesia. The results indicated that N. sativa oil supplementation resulted in significant (P < 0.05) increase of RBCs, hematocrit, WBCs and plasma total protein values in addition to significant hypoglycemia compared to control. The quality of oocytes and reproductive performances were improved due to N. sativa oil supplementation. N. sativa oil rescued the depression in oocytes number and quality after ovarian transplantation. N. sativa oil could not improve hypothermia and hyperglycemia due to general anesthesia. In conclusion, supplementation of N. sativa oil could benefit animals’ health and reproduction through the improvement of physiological parameters and oocyte quality.