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Molecular Cloning and Function Characterization in Feeding of Neuropeptide Y in Gibel Carp, Carassius auratus gibelio

Molecular Cloning and Function Characterization in Feeding of Neuropeptide Y in Gibel Carp, Carassius auratus gibelio

Luo Lei1, Xingxing Deng1, Dengyue Yuan2, Zonglin Zheng1, Chengke Zhu1, Hui Luo1, Baohai Li3, Hua Ye1,* and Chaowei Zhou1,3,*

1College of Animal Sciences, Southwest University, Chongqing 402460, People’s Republic of China
2Department of Aquaculture, College of Life Sciences, Neijiang Normal University, Neijiang, Sichuan, 641000, People’s Republic of China
3Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, People’s Republic of China

Luo Lei and Xingxing Deng have contributed equally to this work.

*      Corresponding authors: 442589528@qq.com; 421807518@qq.com

 

ABSTRACT

To elucidate the mechanism of neuropeptide Y (npy) gene in feeding in gibel carp, we cloned and identified the npy cDNA sequence. Furthermore, we examined the npy mRNA distribution in 21 tissues and investigated the effect of its expression at different nutritional levels. Herein, we found that gibel carp npy has an Open Reading Frame (ORF) containing 291 bp. Moreover, the expression of npy was detected in all tested tissues, with the hypothalamus showing the highest expression in gibel carp. The npy mRNA expression in the hypothalamus significantly decreased 1 and 3 h after feeding (P < 0.01). Furthermore, its expression in the hypothalamus was significantly increased after fasting for 7 days and declined 2 days after refeeding (at 11-day and 14 -day; P < 0.01), suggesting that npy is an orexigenic peptide in gibel carp. In conclusion, these results indicated that npy is a conserved peptide within vertebrates and might exert orexigenic role in gibel carp.
 

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Pakistan Journal of Zoology

April

Vol. 51, Iss. 2, Pages 399-799

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