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Molecular Cloning and Expression of Taste Receptor Gene T2R1 of Obscure Puffer, Takifugu fasciatus

Molecular Cloning and Expression of Taste Receptor Gene T2R1 of Obscure Puffer, Takifugu fasciatus

Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and  Kui Chen2,*

1Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Road, Wuxi 214081, China
2School of Life Sciences, Soochow University, Suzhou 215123, China

*      Corresponding author: chenk@suda.edu.cn;  liub@ffrc.cn

Fig 1

Amplification of taste receptor T2R1 gene of Takifugu fasciatus.

Fig 2

Relative expression of T2R1 in different tissues of Takifugu fasciatus, A, gill; B, spleen; C, heart; D, lips; E, skin; F, tongue.

Fig 3

The similarities of T2R1 amino acid sequences between T. fasciatus with other animals. Note: the amino acids are numbered along the left and right margin. The transmembrane domains are underlined.

Fig 4

Phylogenetic tree revealing the relationship of T2R1 in T. fasciatus relative to other T2Rs family members of other species. Note: the horizontal branch length is proportional to amino acid substitution rate per site. The numbers represent the frequencies (%) with the tree topology presented was replicated after 1000 iterations.

Fig 5

The structural domain of T. fasciatus T2R1 protein
Note: transmembrane region 1. 10-32bp; 2. 39- 61bp; 3. 81-103bp; 4. 124- 146bp; 5. 179- 201bp; 6. 222- 244bp; 7. 259- 278bp

Fig 6

Signal peptide analysis of T2R1 protein of T. fasciatus.

Fig 7

Transmembrane helices prediction of T2R1 by TMpred online tool.

Pakistan Journal of Zoology (Associated Journals)

August

Vol. 49, Iss. 4, Pages 1151-1546

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