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Molecular Characterization of Cry11 Crystal Protein Gene from Bacillus thuringiensis Isolated from Different Soil Samples

Molecular Characterization of Cry11 Crystal Protein Gene from Bacillus thuringiensis Isolated from Different Soil Samples

Dilara A. Bukhari1,*, Naureen Fatima1 and Abdul Rehman2

 

1Department of Zoology, Government College University, Lahore
2Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590

*      Corresponding author: dilaraabbas@yahoo.com

 

ABSTRACT

The present study was aimed at screening the local Bacilllus thuringiensis (B.t.) isolates for Cry11 and assess their potential use for mosquito control. A total of 15 B.t. isolates were collected, of which 75% were from dry, leaf litter, garden soil samples, 15% were from animal waste and 10 % were from moist soil of the crop area. A 650bp of cry11 gene was amplified by PCR and seven isolateswere found positive for cry11 gene. The 16S rDNA study exposed that these screened B.t. confirmed 99% homology with B.t. serovar tolworthi, B.t. str. Al Hakam, B.t. serover thuringiensis, B.t. serovar konkukian, B.t. serovar Chinensis. B.t. serover Indiana, and B.t. serover kurstuki. The toxicity bioassays with B.t. spores proved that seven B.t. isolates harboring cry11 gene (viz., NF B.t.,1,2,3,4,5,6,7) were toxic to 3rd instar larvae of mosquito, Aedes aegypti. Among seven B.t. isolates, three isolates NF5 B.t. 7.2, NF1 B.t. 1.1 and NF2 B.t. 4.2 were found most toxic which were isolated from moist soil containing decaying cattle waste, dry waste animal dung and leaf litter soil, respectively. So, these isolates have a great potential to grow into a biopesticidal formulation for control of mosquitoes.
 

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Pakistan Journal of Zoology

December

Vol. 50, Iss. 6, Pages 1999-2398

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