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Immunolocalization and Expression of Androgen Receptor in the Oviduct of Laying Hens

Immunolocalization and Expression of Androgen Receptor in the Oviduct of Laying Hens

Bian Xunguang1,2, Li Jiancheng1, Xu Chu1 and Yang Li2,3,4,*

1School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng, Jiangsu Province, People’s Republic of China
2Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng Teachers University, Yancheng 224051, Jiangsu Province, People’s Republic of China
3Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Yancheng Teachers University, Yancheng, Jiangsu Province, People’s Republic of China
4Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng, Jiangsu Province, People’s Republic of China

*      Corresponding author: bxguang8311@126.com

Fig. 1.

Light micrographs in different regions of the hens oviduct. A, infundibulum; B, magnum; C, isthmus; D, uterus; E, vagina; F, the SST in the anterior vagina. Ep, epithelium; Gl, gland; Lu, lumen; St, sperm-storage tubul; Mu, muscle; Bv, blood vessel; Se, serous. Bar=100 μm (A), bar=500 μm (D), bar=200 μm (B, E), bar=50 μm (C, F).

Fig. 2. 

Relative mRNA expression levels of AR in different regions of oviduct, determined by qPCR. The β-actin gene was used as an internal standard. Different letters indicate significant differences between regions (p<0.05). Values represent means ± SE (n=5).

Fig. 3.

Immunostaining of AR in five regions of the hens oviduct. A and B, infundibulum; C, magnum; D, isthmus; E, uterus; F, vagina. Ep, epithelium; Gl, gland; Lu, lumen; Bv, blood vessel. Bar=100 μm (A, D); bar=50 μm (B, C, E, F).

Pakistan Journal of Zoology

December

Vol. 49, Iss. 6, Pages 1937-2341

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