Immunoglobulin G and Total Protein Concentration in Blood and Colostrum of Different Cattle Breeds and its Passive Transfer to Neonatal Calves

Muhammad Shafiq1,2,*, Rajwali Khan1, Ilyas Ali3, Sadeeq Ur Rahman4, Saif Ullah3,

Shah Jan Mohammad2, Mohammad Jan2 and Jinhu Huang2

1Department of Livestock Management and Animal Breeding and Genetics, Faculty of Animal Husbandry and Veterinary Sciences, University of Agriculture, Peshawar, Pakistan

2College of Veterinary Medicine, Nanjing Agriculture University, Nanjing, People Republic of China

3College of Animal Science and Technology, Nanjing Agriculture University, Nanjing, People Republic of China

4College of Veterinary Sciences and Animal Husbandry, Section Microbiology, Abdul Wali Khan University, Mardan, Khyber Pakhtunkhwa, Pakistan

ABSTRACT

The aim of this study was to determine the effect of cattle breed type on (a) cow serum IgG and serum total protein concentration (b) colostrum immunoglobulin level and (c) their respective calves’ serum immunoglobulin and serum total protein concentration. Three breeds of cattle were observed: Jersey, Holstein Friesian (HF) and local Pakistani cow breed Achai. To assess serum IgG, sodium sulphite precipitation technique was used while IgG in colostrum were determined using digital Brix refractometer (Atago RX-1000) and serum total protein (STP) in cows and calves were analyzed by chemistry analyzer (Procan PS-520). Overall Achai breed of cattle showed highest mean value for pre and postpartum serum immunoglobulin (11.78 ± 0.92 mg/ml, 10.00 ± 1.09 mg/ml) than Jersey breed (9.80 ± 1.30 mg/ml, 7.86 ± 1.01 mg/ml) and HF breed (7.86 ± 1.30 mg/ml, 6.43 ± 0.92mg/ml) respectively. Calf serum IgG was higher (P < 0.05) for Achai cow breed than other two cow breeds. The mean Brix (%) value for colostrum of Achai breed was higher (24.03% Brix) than Jersey and HF breeds. In the current study, there was a positive significant (P<0.05) correlation found for pre-partum IgG with postpartum STP and calf serum IgG. The results revealed that Achai breed had better potentials for serum and colostrum immunoglobulins (IgG) and total proteins (STP) production under local environment than HF and Jersey. Calf serum immunoglobulin concentration was affected by cattle breed type and showed a positive correlation with cow serum IgG decrease before parturition. It was found that the Partum stage of different cattle breeds has a significant effect on IgG in colostrum and serum of their respective calves. Therefore, the present study provided a baseline for the improvement of passive transfer of immunity to newborn calves while manipulating IgG concentration in the pre-partum stage of cattle.

Article Information

Received 02 June 2019

Revised 30 July 2019

Accepted 17 September 2019

Available online 22 February 2021

Authors’ Contribution

MS and RK designed the study and planned the experiments. IA, JH, SUR and SU helped in the analysis. MS wrote the manuscript. RK, SJM and MJ revised the manuscript.

Key words

Refractometer, Achai, Pre-partum, Post-partum, IgG

DOI: https://dx.doi.org/10.17582/journal.pjz/20190602170613

* Corresponding author: [email protected]

0030-9923/2021/0002-0653 $ 9.00/0

Copyright 2021 Zoological Society of Pakistan

INTRODUCTION

Calf is one of the saleable products in dairy industry and therefore a basic management objective is to make sure that the calf survives and remains healthy. Tolerable resistance to infection after post-parturient period is vital for the health and existence of a calf (Woods and Roussel, 1993). Neonatal calf morbidity and mortality are the major economic losses to livestock industry. In Peshawar commercial dairy farms (17.98%) neonatal calves’ mortality in buffalo and calves were reported by (Khan et al., 2007). Mortality rate in cattle and buffalo calves in Pakistan ranged from 29.1% to 39.8% (Afzal et al., 1983). (Martin) reported that calf mortality of 20% decrease the profit of livestock farm to 38%. The main cause of high mortality in commercial dairy farms is the inadequate feeding and health facilities like colostrum feeding, inadequate milk feeding, naval cord disinfection and timely treatment (Tiwari et al., 2007). Calf mortality can only be reduced by finding and targeting its specific reasons (Khan and Khan, 1995).

Neo-born calves in cattle are born lacking of circulating immunoglobulins, because of syndesmochorial type of placenta and due to the placental barriers the larger immunoglobulin G cannot pass through placenta so the neonatal calves achieve their passive immunity by feeding maternal colostrum (Blum and Baumrucker, 2008). It has been described by (Hernandez-Castellano et al., 2015), that the immune status can influence the transfer of immune components from blood to colostrum. According to (Arthington et al., 2000) unsucckled neonatal calves’ serum IgG concentration is enormously low (<0.1 g/L). The ability of the new-born calf to absorb colostral IgG decreases rapidly after birth. Therefore, they must absorb maternal immunoglobulins via intestine from colostrum for passive immunity during the neonatal period. Transport of these immunoglobulins from maternal serum to colostrum starts 5 weeks prior to parturition and reach to its optimum peak level at 1 to 3 days before parturition while cessation of these immunoglobulins occur immediately just after parturition (Sasaki et al., 1976).

Effects of breed have been reported for blood immunoglobulin’s concentration in cow serum (Norman et al., 1981; McGee, 1997; Guy et al., 1994) and serum immunoglobulin G concentration of calves (Vann et al., 1995a). Breed effects have also been reported for colostrum immunoglobulin concentration (Muller and Ellinger, 1981). They revealed that HF and Jersey breeds have significant effect on colostrum immunoglobulin G concentration compared with Arshyre cows. According to the results obtained by (Hernandez-Castellano et al., 2015; Poulsen et al., 2010; Tyler et al., 1996) if neonatal calf does not receive sufficient amount of colostrum or if the colostrum is deprived of immunoglobulin’s, then the new born calf may suffer from the failure of passive immunity transfer (FPIT). Failure of passive immunity transfer is defined as that serum which have less than 10 mg/ml of immunoglobulin G (IgG) or calf serum which have less than 5.2 gm/dL serum total protein (STP) (Calloway et al., 2002; Moore et al., 2005; Tyler et al., 1996). The objective of this study was to determine the effect of cattle breed type on (a) cow serum and colostrum immunoglobulin level and (b) their respective calves’ serum immunoglobulin concentration.

MATERIALS AND METHODS

Ethics statement

This study has been reviewed and approved by the institutional animal care and use committee of the University of Agriculture Peshawar, Faculty of Animal Husbandry and Veterinary Sciences, and was performed in accordance with the relevant guidelines and regulations.

Animals selection and sampling

A total of 168 mature advance pregnant cows were selected of Achai, Jersey and Holstein Friesian (HF) cattle breeds. Achai are small-sized dairy cattle breed found in Khyber Pakhtunkhwa province and are famous for its high resistant to extreme environmental conditions. Each breed of cattle group has been comprised of 56 cows with parity range from 2-5 with known expected dates of parturitions. The samples were collected from Cattle Breeding and Dairy Farm Hari Chand. Serum and colostrum samples were collected from April 2015 to September 2015. Two times of 5ml blood samples were collected from 168 cows at pre-partum (15 days prior to parturition) and post-partum stages (within 2 h of parturition) with no (EDTA) solution in test tubes. Immediately after post-partum and prior to suckling a 20 ml of fresh-pooled colostrum were collected from the right front quarter or hindquarter of the udder. The colostrum samples were placed in plastic bottles labeled with each cow identification number. Calves were fed whole milk at a rate of 5% of birth weight, twice daily. After calving, the calves received 1.5 liters of colostrum from their mother through teat bottle. After colostrum feeding to neonatal calves 5ml of blood samples were collected in test tubes with no anticoagulant from a jugular vein after 24 hours of their 1st colostrum feeding.

Centrifugation and transportation of samples

After collection of all blood samples, were then kept undisturbed at room temperature for 15 to 30 minutes for clot formation. Then the samples were centrifuged at 3,000 rpm for 15 minutes. Serum and colostrum samples were collected with universal safety measures and put into leak proof container. Samples were properly packed, labeled with a specific codes and collection date and kept in a container having dry ice pieces. The container was sent within 2 h to laboratory. All samples were transported to Pathology lab at UAP and kept frozen at -20˚Cuntil analysis.

Immunological measurements of serum

Analysis of immunoglobulin G (IgG) in cow and calves’ serum were performed by sodium sulphite precipitation method as described by (Dawes et al., 2002). This test uses 14%, 16% and 18% sodium sulphite solutions prepared by dissolving 14, 16 and 18 gm of anhydrous salt each in a total volume of 100 ml distilled water. Then 9 ml of each solution samples were added in three test tubes. Finally, 0.9 ml of serum sample were added, in each test tube and properly mixed, then allowed to stand undisturbed for 30 min.

Analysis of serum total protein

For estimation of total protein (TP) in serum samples Chemistry Analyzer (Procan PS-520) and protein kit (Human Total Protein liquid color) was used. Serum total proteins (STP) analyzed by the Biuret method as described by (Josephson and Gyllensward, 1957).

Colostrum analysis through brix refractometer

Analysis of IgG in colostrum samples digital brix refractometer (ATAGO RX-1000) used to assess failure of passive transfer (FPT). For the digital Brix refractometer, approximate 250 µL of colostrum sample were used, and then the Brix score of the liquid determined by shining a light through the sample in the prism, measuring the refraction index, and representing the reading (%Brix) on a digital scale (Bielmann et al., 2010; Quigley et al., 2013).

Statistical analysis of data

The data was statistically analyzed for the effect of breed and partum stage on serum immunoglobulin and %Brix of colostrum using General linear model (GLM) technique in statistical program (SAS, 2002). Analysis of variance was carried out using PROC of SAS (SAS, 2002). The means compared by Duncan multiple range test (DMR). Computer software Excel (Microsoft 2016) was used for storage of data. Values considered significant when P < 0.05.

RESULTS

The immunoglobulin (IgG) concentration in serum samples of cows and calves.

The data of serum IgG concentration of cows with different partum stages and calves at 24 h after colostrum feeding is presented in Table I. In prepartum stage of cattle, the highest mean concentration of IgG was found in Achai breed of cattle followed by Jersey breed while lowest mean concentration was found in Holstein Friesian breed of cattle. Breed effect on postpartum serum IgG was found significant (P<0.05). Mean value of IgG for Achai breed of cattle was higher than the mean value of Jersey breeds of cattle, while the lowest concentration was recorded in Holstein Friesian breed of cattle. Effect of breed on calf serum immunoglobulin was found significant (P<0.05) as shown in Table I. The mean value of IgG in Achai calves showed highest concentration followed by Jersey calves and the lowest mean value of IgG was found in HF calves.

Serum total protein (STP) concentration of cows and calves and Brix% values of fresh pooled colostrum.

The data of STP concentration in serum samples of cows and calves and Brix% values of colostrum is presented in Table II. Effect of breed have found highly significant (P<0.001) on prepartum and postpartum STP. Highest mean value of serum total protein was found for Achai breed followed by Jersey breed and the lowest mean value was found for Holstein Friesian breed of cattle at pre and postpartum stages respectively. While the effect of breed on calf serum total protein was found highly significant (P<0.05) for calves’ serum total protein. Mean value for Jersey calves was highest in concentration followed by Achai calves and the lowest mean value was found in HF.

For the estimation of colostrum IgG Brix refractometer was used. The data of Brix (%) values are presented in Table II. Similarly effect of breed on colostrum IgG %Brix value was found significant (P<0.05). Mean %Brix value of colostrum in Achai breed of cattle was found highest, second highest %Brix value was found for Jersey breed of cattle, while the lowest mean value was found for HF breed of cattle.

 

Table I. The immunoglobulin (IgG) concentration (mg/ml) (Mean±SEM) in serum samples of cows and calves.

Breed	Prepartum serum IgG in Cows	Postpartum serum IgG in Cows	Calves serum IgG at 24 h
Jersey	9.80 ± 0.92	7.86 ± 1.01	11.43 ± 0.92
HF	7.86 ± 1.30	6.43 ± 0.92	9.29 ± 1.30
Achai	11.78 ± 0.92	10.00 ± 1.09	12.14 ± 1.49
P-Value	<0.05	<0.05	<0.05

 

Abbreviations: IgG, immunoglobulin G; SEM, standard error of mean; significant difference (P<0.05).

 

DISCUSSION

The transition period is considered the most critical and challenging for the dam health status during the lactation cycle (Hernandez-Castellano et al., 2017) and (Kessel et al., 2008). In the present study effect of different breeds was evaluated on cow serum IgG and serum total protein concentration, colostrum IgG and their respective calves’ serum immunoglobulin and serum total protein concentration. The findings of the present study are in consistent with the findings of (Murphy et al., 2009) and (Bayram et al., 2016). They studied that there is significant variation of breed in the concentration of pre-partum serum immunoglobulin G. According to Hernandez-Castellano et al. (2018) and Barrington et al. (1997) variations are present in serum immunoglobulin’s concentration for different cattle breeds. The high concentration of serum immunoglobulin concentration in Achai breed may be due to their high adoptability and acclimatization to local

 

Table II. Serum total protein (STP) concentration (mg/ml) (Mean±SEM) of cows and calves and Brix% values of fresh pooled colostrum.

Breed	Prepartum serum total protein (STP) in Cow	Postpartum serum total protein (STP) in Cows	Calves serum total protein (STP) at 24 h	Colostrum Brix (%) for Cow
Jersey	5.71 ± 0.42	5.28 ± 0.26	7.57 ± 0.37	21.84 ± 1.05
HF	5.28 ± 0.46	4.85 ± 0.57	5.00 ± 0.53	17.13 ± 1.49
Achai	7.28 ± 0.58	7.00 ± 0.48	7.14 ± 0.51	24.03 ± 0.72
P-Value	<0.05	<0.05	<0.05	<0.05

 

Abbreviations: STP, serum total protein; SEM, standard error of mean; significant difference (P<0.05).

 

environment compared with HF and Jersey breed of cattle. The current study is in consistent with the arguments of (Tao and Dahl, 2013). They studied that high ambient temperature effect the performance of dairy cattle’s. The present findings are in line with the results of (Hernandez-Castellano et al., 2017; Logan et al.,1981; Shell et al., 1995). Their results show that immunoglobulin G concentration in serum of cow decreases after 1-day postpartum and relatively low up to 2 to 4 weeks. (Norman et al., 1981) also justify our findings. He found that breed differences are present in cow serum IgG concentration.

The current results of serum total protein are similar with the previous findings (Calloway et al., 2002; Tyler et al., 1996). They recommended that serum total protein cut-point ranges of 5.0 to 5.5 gm/dL equal to serum immunoglobulin G level of 8.9 to 13.4mg/ml (Tyler et al., 1998) found that serum total protein below 5.0m/dL will be at risk of failure of passive immunity transfer. Previous studies of Ozcelik et al. (2017), Ali et al. (2019) and Tao and Dahl (2013) showed that performance of dairy animals are affected by several factors including season, latitude. The high concentration of serum total protein concentration in Achai breed may be due to their high adoptability and familiarization to native environment compared with HF and Jersey breed of cattle.

Ambient temperature and stress are important factors in the absorption of immunoglobulin and total protein in newborn calves. The present findings are in line with the arguments of Campbell et al. (2007). They opined that environmental stress has a significant effect on the absorption of immunoglobulin and total protein in newborn calves. The probable reason of high serum immunoglobulin in newborn Achai calves may be due to their adoptability to local environment. These finding supports the study of Deelen et al. (2014). He concluded that the calves of Jersey cattle had highest concentration of serum total protein (4.4 to 8.8 g/dl). Benavides-Varela et al. (2013) also studied the concentration of total serum protein in calves. He concluded that the calves of HF breed have lowest concentration of serum total protein (5.4 g/dl) compared with other cross breed. Villarroel et al. (2013) results shows that Jersey calves have significantly higher serum total protein than HF calve. Tendency exist that Jersey breed calves have higher STP concentration than HF calves after ingestion of colostrum (Tennant et al., 1969).

Effect of breed on colostrum IgG was not significant (P>0.05). Mean value for jersey breed was found highest, second highest mean value of colostrum for Achai breed of cattle while mean value for HF cattle breed was lowest than the other two breed. These findings are in line with the findings of Muller and Ellinger (1981). They concluded that there is no significant effect of breed on colostrum IgG however trends were existed that Jersey breed having highest IgG concentration. Similarly, Hernandez-Castellano et al. (2017) and (Hernandez-Castellano et al., 2018) also concluded that colostrum IgG in did not differ between two groups of cattle throughout the experimental period. (Vann et al., 1995b) observed the same findings that any breed type did not affect IgG concentration in colostrum. However, breed variations for colostrum immunoglobulin G in Holstein Friesian were found by (Pritchett et al., 1991) and in Jersey Cows (Logan et al., 1981).

The present study mean Brix% for HF cow’s colostrum is similar to the findings of Quigley et al. (2013) and Hernandez et al. (2016). He concluded that when Brix refractometer is used for colostrum IgG than 21% Brix will be measured the break point for high quality (>50 gm of IgG/L) maternal colostrum. However, variation may exist in colostrum Brix% value among different cattle breeds (Morrill et al., 2012; Bielmann et al., 2010). Their findings of Brix% values for maternal colostrum are identical in line with the current study’s findings. Brix % for Achai was found higher than other two breeds. There are many possible explanations for the difference between brix% reported in the present study and previous studies. In previous studies of Morrill et al. (2012), Bielmann et al. (2010), Quigley et al. (2013) a wide range of environmental conditions, feeding practices and management were involved which are known to affect colostrum quality. In the current study 168 milking animals were studied whereas the Morrill et al. (2012) having flock size of 80 to 5000 dairy cows were studied. Previous studies show that time variation in colostrum collection may also affect colostrum brix%. Chigerwe et al. (2008) and Moore et al. (2005) showed that colostrum collected more than 2 h after parturition than brix% in colostrum is significantly lower than the early collection.

CONCLUSION

In this study, the partum stage of different cattle breeds has a significant effect on IgG and total protein in colostrum and serum of their respective calves. Therefore, the current study provided a base line for the enhancement of passive transfer of immunity to newborn calves while manipulating IgG andserum total protein level in the pre-partum stage of cattle. Similarly, it was concluded that Achai and Jersey calves were in low risk for failure of immunity transfer (>10 mg/ml of IgG) while in HF calves medium risk of failure of immunity transfer (<10 mg/ml) was found. Brix refract meter was used among different cow breeds for colostrum IgG analysis which was considered easy and good indicator for IgG estimation in the field.

RECOMMENDATIONS

1. Research needed to be conducted to find out the sources of IgG and total protein supplementation for the cattle’s in advance pregnant stages.
2. IgG estimation of cow and calves’ serum through sodium sulfite precipitation test and serum total protein was considered good indicators of passive immunity in calves at laboratory.
3. Brix refractometer for colostrum analysis is considered easy and good indicator for estimation of passive immunity transfer.
4. In the current study HF calves were on risk due to low serum IgG in their dam serum and colostrum so supplementation is needed to the dam at advance pregnancy or treated colostrum is given to their calves to prevent from the failure of passive immunity transfer.

ACKNOWLEDGEMENTS

This work was supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). We would like to acknowledge Dr. Frank (The University of Bradford, UK) for critical reading and editing this manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

REFERENCES

Afzal, M., Javed, M.H. and Anjum, A.D., 1983. Calf mortality: seasonal pattern, age distribution and causes of mortality (buffaloes, dairy cattle). Pakistan Vet. J., 3: 30-33..

Ali, I., Suhail, S.M. and Shafiq, M., 2019. Heritability estimates and genetic correlations of various production and reproductive traits of different grades of dairy cattle reared under subtropical condition. Reprod. Domest. Anim., 54: 1026– 1033. https://doi.org/10.1111/rda.13458

Arthington, J.D., Cattell, M.B. and Quigley, J.D., 3rd. 2000. Effect of dietary IgG source (colostrum, serum, or milk-derived supplement) on the efficiency of Ig absorption in newborn Holstein calves. J. Dairy Sci., 83: 1463-1467. https://doi.org/10.3168/jds.S0022-0302(00)75018-1

Barrington, G.M., Besser, T.E., Davis, W.C., Gay, C.C., Reeves, J.J., and McFadden, T.B., 1997. Expression of immunoglobulin G1 receptors by bovine mammary epithelial cells and mammary leukocytes. J. Dairy Sci., 80: 86-93. https://doi.org/10.3168/jds.S0022-0302(97)75915-0

Bayram, B., Aksakal, V., Turan, I., Demir, S., Mazlum, H. and Coşar, I., 2016. Comparison of immunoglobulin (IgG, IgM) concentrations in calves raised under organic and conventional conditions. Indian J. Anim. Res. 50: 995-999. https://doi.org/10.18805/ijar.11472

Benavides-Varela, D., Elizondo-Salazar, J.A. and González-Arias, E., 2013. Immune status of dairy calves in the Northern Plains of Costa Rica. Year II. Agron. Mesoam., 24: 285-291. https://doi.org/10.15517/am.v24i2.12527

Bielmann, V., Gillan, J., Perkins, N.R., Skidmore, A.L., Godden, S. and Leslie, K.E., 2010. An evaluation of Brix refractometry instruments for measurement of colostrum quality in dairy cattle. J. Dairy Sci., 93: 3713-3721. https://doi.org/10.3168/jds.2009-2943

Blum, J.W. and Baumrucker, C.R., 2008. Insulin-like growth factors (IGFs), IGF binding proteins, and other endocrine factors in milk: role in the newborn. Adv. Exp. Med. Biol., 606: 397-422. https://doi.org/10.1007/978-0-387-74087-4_16

Calloway, C.D., Tyler, J.W., Tessman, R.K., Hostetler, D. and Holle, J., 2002. Comparison of refractometers and test endpoints in the measurement of serum protein concentration to assess passive transfer status in calves. J. Am. Vet. Med. Assoc., 221:1605-1608. https://doi.org/10.2460/javma.2002.221.1605

Campbell, J.M., Russell, L.E., Crenshaw, J.D., Weaver, E.M., Godden, S., Quigley, J.D., Coverdale, J., and Tyler, H., 2007. Impact of irradiation and immunoglobulin G concentration on absorption of protein and immunoglobulin G in calves fed colostrum replacer. J. Dairy Sci., 90: 5726-5731. https://doi.org/10.3168/jds.2007-0151

Chigerwe, Munashe, Tyler, J.W., Schultz, L.G., Middleton, J.R., Steevens, B.J. and Spain. J.N., 2008. Effect of colostrum administration by use of oroesophageal intubation on serum IgG concentrations in Holstein bull calves. Am. J. Vet. Res. 69: 1158-1163. https://doi.org/10.2460/ajvr.69.9.1158

Dawes, M.E., Tyler, J.W., Hostetler, D., Lakritz, J. and Tessman, R., 2002. Evaluation of a commercially available immunoassay for assessing adequacy of passive transfer in calves. J. Am. Vet. Med. Assoc. 220 : 791-793. https://doi.org/10.2460/javma.2002.220.791

Deelen, S.M., Ollivett, T.L., Haines, D.M. and Leslie, K.E., 2014. Evaluation of a brix refractometer to estimate serum immunoglobulin G concentration in neonatal dairy calves. J. Dairy Sci., 97: 3838-3844. https://doi.org/10.3168/jds.2014-7939

Guy, M.A., McFadden, T.B., Cockrell, D.C. and Besser, T.E., 1994. Regulation of colostrum formation in beef and dairy cows. J. Dairy Sci., 77: 3002-3007. https://doi.org/10.3168/jds.S0022-0302(94)77241-6

Hernandez-Castellano, L.E., Hernandez L.L., Sauerwein, H. and Bruckmaier, R.M., 2017. Endocrine and metabolic changes in transition dairy cows are affected by prepartum infusions of a serotonin precursor. J. Dairy Sci., 100: 5050-5057. https://doi.org/10.3168/jds.2016-12441

Hernandez-Castellano, L.E., Hernandez, L.L., Weaver, S. and Bruckmaier, R.M., 2017. Increased serum serotonin improves parturient calcium homeostasis in dairy cows. J. Dairy Sci., 100: 1580-1587. https://doi.org/10.3168/jds.2016-11638

Hernandez-Castellano, L.E., Morales-delaNuez, A., Sanchez-Macias, D., Moreno-Indias, I., Torres, A., Capote, J., Arguello, A. and Castro, N., 2015. The effect of colostrum source (goat vs. sheep) and timing of the first colostrum feeding (2h vs. 14h after birth) on body weight and immune status of artificially reared newborn lambs. J. Dairy Sci., 98: 204-210. https://doi.org/10.3168/jds.2014-8350

Hernandez-Castellano, L.E., Ozcelik, R., Hernandez, L.L. and Bruckmaier, R.M., 2018. Short communication: Supplementation of colostrum and milk with 5-hydroxy-l-tryptophan affects immune factors but not growth performance in newborn calves. J. Dairy Sci., 101:794-800. https://doi.org/10.3168/jds.2017-13501

Hernandez, D., Nydam, D.V., Godden, S.M., Bristol, L.S., Kryzer, A., Ranum, J. and Schaefer, D., 2016. Brix refractometry in serum as a measure of failure of passive transfer compared to measured immunoglobulin G and total protein by refractometry in serum from dairy calves. Vet. J., 211:82-87. https://doi.org/10.1016/j.tvjl.2015.11.004

Josephson, B., and Gyllensward, C., 1957. The development of the protein fractions and of cholesterol concentration in the serum of normal infants and children. Scand. J. Clin. Lab. Invest., 9: 29-38. https://doi.org/10.3109/00365515709088110

Kessel, S., Stroehl, M., Meyer, H.H., Hiss, S., Sauerwein, H., Schwarz, F.J. and Bruckmaier, R.M., 2008. Individual variability in physiological adaptation to metabolic stress during early lactation in dairy cows kept under equal conditions. J. Anim. Sci., 86: 2903-2912. https://doi.org/10.2527/jas.2008-1016

Khan, A., and Khan, M.Z., 1995. Epidemiological aspects of neonatal calf mortality in the Nili-Ravi buffalo (Bubalus bubalis). Pakistan Vet. J., 15:163-168.

Khan, Z.U., Khan, S., Ahmad, N. and Raziq, A., 2007. Investigation of mortality incidence and managemental practices in buffalo calves at commercial dairy farms in Peshawar City. J. Agric. Biol. Sci., 2: 16-22.

Logan, E.F., Meneely, D.J. and Lindsay, A., 1981. Colostrum and serum immunoglobulin levels in Jersey cattle. Br. Vet. J., 137: 279-282. https://doi.org/10.1016/S0007-1935(17)31689-5

Martin, SW., Wiggin. 1973. Perinatal diseases. Am. J. Vet. Res., 34: 107-112.

McGee, M., 1997. Defining suckler systems in terms of efficiency of lean meat production and market requuirements. University College Dublin.

Moore, M., Tyler, J.W., Chigerwe, M., Dawes, M.E. and Middleton, J.R., 2005. Effect of delayed colostrum collection on colostral IgG concentration in dairy cows. J. Am. Vet. Med. Assoc., 226: 1375-1377. https://doi.org/10.2460/javma.2005.226.1375

Morrill, K.M., Conrad, E., Polo, J., Lago, A., Campbell, J., Quigley, J. and Tyler, H., 2012. Estimate of colostral immunoglobulin G concentration using refractometry without or with caprylic acid fractionation. J. Dairy Sci., 95: 3987-3996. https://doi.org/10.3168/jds.2011-5104

Muller, L.D. and D.K. Ellinger. 1981. Colostral immunoglobulin concentrations among breeds of dairy cattle. J. Dairy Sci., 64: 1727-1730. https://doi.org/10.3168/jds.S0022-0302(81)82754-3

Murphy, B.M., Drennan, M.J, O’Mara, F.P. and Earley, B., 2005. Cow serum and colostrum immunoglobulin (IgG1) concentration of five suckler cow breed types and subsequent immune status of their calves. Irish J. Agric. Fd Res., 44: 205–212.

Norman, L.M., Hohenboken, W.D. and Kelley, K.W., 1981. Genetic differences in concentration of immunoglobulins G1 and M in serum and colostrum of cows and in serum of neonatal calves. J. Anim. Sci., 53: 1465-1472. https://doi.org/10.2527/jas1982.5361465x

Ozcelik, R., Bruckmaier, R.M. and Hernandez-Castellano, L.E., 2017. Prepartum daylight exposure increases serum calcium concentrations in dairy cows at the onset of lactation. J. Anim. Sci., 95: 4440-4447. https://doi.org/10.2527/jas2017.1834

Poulsen, K.P., Foley, A. L., Collins, M.T. and McGuirk, S.M., 2010. Comparison of passive transfer of immunity in neonatal dairy calves fed colostrum or bovine serum-based colostrum replacement and colostrum supplement products. J. Am. Vet. Med. Assoc., 237: 949-954. https://doi.org/10.2460/javma.237.8.949

Pritchett, L.C., Gay, C.C., Besser, T.E. and Hancock, D.D., 1991. Management and production factors influencing immunoglobulin G1 concentration in colostrum from Holstein cows. J. Dairy Sci., 74: 2336-2341. https://doi.org/10.3168/jds.S0022-0302(91)78406-3

Quigley, J.D., Lago, A., Chapman, C., Erickson, P. and Polo, J.. 2013. Evaluation of the Brix refractometer to estimate immunoglobulin G concentration in bovine colostrum. J. Dairy Sci., 96: 1148-1155. https://doi.org/10.3168/jds.2012-5823

Sasaki, M., Davis, C.L. and Larson, B.L., 1976. Production and turnover of IgG1 and IgG2 immunoglobulins in the bovine around parturition. J. Dairy Sci., 59: 2046-2055. https://doi.org/10.3168/jds.S0022-0302(76)84486-4

Shell, T.M., Early, R.J., Carpenter, J.R. and Buckley, B.A., 1995. Prepartum nutrition and solar radiation in beef cattle: II. Residual effects on postpartum milk yield, immunoglobulin, and calf growth. J. Anim. Sci., 73 : 1303-1309. https://doi.org/10.2527/1995.7351303x

Tao, S. and Dahl, G.E., 2013. Invited review: heat stress effects during late gestation on dry cows and their calves. J. Dairy Sci., 96: 4079-4093. https://doi.org/10.3168/jds.2012-6278

Tennant, B., Harrold, D., Reina-Guerra, M. and Laben, B.C., 1969. Neonatal alterations in serum gamma globulin levels of Jersey and Holstein-Friesian calves. Am. J. Vet. Res., 30: 345-354.

Tiwari, R., Sharma, M. and Singh. 2007. Buffalo calf health care in commercial dairy farms: a field study in Uttar Pradesh (India). Livest. Res. Rural Develop., 19: 8.

Tyler, J.W., Hancock, D.D., Parish, S.M., Rea, D.E., Besser, T.E., Sanders, S.G. and Wilson, L.K., 1996. Evaluation of 3 assays for failure of passive transfer in calves. J. Vet. Intern. Med., 10: 304-307. https://doi.org/10.1111/j.1939-1676.1996.tb02067.x

Tyler, J.W., Hancock, D.D., Wiksie, S.E., Holler, S.L., Gay, J.M. and Gay, C.C., 1998. Use of serum protein concentration to predict mortality in mixed-source dairy replacement heifers. J. Vet. Intern. Med., 12: 79-83. https://doi.org/10.1111/j.1939-1676.1998.tb02099.x

Vann, R.C., Holloway, J.W., Carstens, G.E., Boyd, M.E. and Randel, R.D., 1995. Influence of calf genotype on colostral immunoglobulins in Bos taurus and Bos indicus cows and serum immunoglobulins in their calves. J. Anim. Sci., 73: 3044-3050. https://doi.org/10.2527/1995.73103044x

Villarroel, A., Miller, T.B., Johnson, E.D., Noyes, K.R. and Ward, J.K., 2013. Factors affecting serum total protein and immunoglobulin G concentration in replacement dairy calves. Adv. Dairy Res., 1: 106-110.

Woods, P.R. and Roussel, A.J., 1993. Colostrum and passive immunity. In: Current veterinary therapy: Food animal practice (ed. J.L. Howard). WB Saunders Co., Philadelphia, pp. 97-100.