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Identification of Differentially Expressed Long Noncoding RNAs and mRNAs Involved with Dominant Follicle Selection in Goats using RNA-seq

Identification of Differentially Expressed Long Noncoding RNAs and mRNAs Involved with Dominant Follicle Selection in Goats using RNA-seq

Guang-Xin E1, Yong-Ju Zhao1, Yue-Hui Ma2, Ming-Xing Chu2, Jia-Hua Zhang1, Zhong-Quan Zhao1, Hui-Jiang Gao2, Huai-Zhi Jiang3, Di Liu4, Li Liu5, Yan-Bin Zhu6, Wang-Dui Basang6, Luo-Bu Danjiu7, Tian-Wu An8, Xiao-Lin Luo8, Shi-Cheng He7 and Yong-Fu Huang1,*

1Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chongqing 400716, China
2Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China
3College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
4Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Science, Harbin 150086, China
5College of Animal Science and Technology, China Agricultural University, Beijing 100083, China
6Tibet Academy of Agriculture and Animal Husbandry Science, Lasa 850001, China
7Nagqu grassland station, Naqu 852000, China
8Sichuan Academy of Grassland Sciences, Chengdu, Sichuan 611731, China

Guang-Xin E and Yong-Ju Zhao contributed equally in this article.

*      Corresponding author: H67738337@swu.edu.cn

 

ABSTRACT

In this study, we used high-throughput technology to provide the first transcriptome dataset for differentially expressed genes in mixed pools of dominant and nondominant follicles of goats. These data will contribute to research on the molecular mechanisms of dominant follicle selection in goats. In this study, 90276370 and 115579236 clear reads in dominant and nondominant follicles of goat were generated through Illumina paired-end sequencing, and their mapping rate was 84.99% and 84.47%, respectively. A total of 12577 differentially expressed genes (DEGs) were identified, including 6009 upregulated and 6568 downregulated genes in dominant follicles compared with nondominant follicles of goat. Of the 1026 significantly differentially expressed, long noncoding RNAs (lncRNAs) found, 419 were upregulated and 607 were downregulated. The DEGs related to 56 GO categories, and pathway analysis revealed that these DEGs were significantly enriched in 41 of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including signaling pathways regulating the pluripotency of stem cells, the p53 signaling pathway, and oxidative phosphorylation. The results of the present study confirmed that the selection of the dominant follicle involved the regulation of various physiological systems. These results provided helpful data to understand on the molecular mechanisms of dominant follicle selection in goats.
 

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Pakistan Journal of Zoology

December

Vol. 49, Iss. 6, Pages 1937-2341

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