Assessment of Sugarcane Genotypes for Cane Yield

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SJA_33_4_668-673

Research Article

Assessment of Sugarcane Genotypes for Cane Yield

Amjad Ali1*, Sher Aslam Khan2, Abid Farid2, Ayub khan2, Shah Masaud Khan2 and Naushad Ali2

1Sugar Crops Research Institute Mardan, Pakistan; 2Department of Agricultural Sciences, University of Haripur, Haripur, Pakistan.

Abstract | Fourteen sugarcane genotypes and two check cultivars were evaluated in randomizedcomplete block design with three replications at Sugar Crops Research Institute Mardan during 2012-13 and 2013-14. The studies were conducted in two plant crops to calculate broad sense heritability (repeatability), genetic gain and correlations among the parameters, and establish selection criteria.Highly significant (p ≤ 0.01) differences were found among the genotypes for number of tillers, plant height, cane length,number of nodes, internodes length,number of millablecane and cane yield while non-significant differences were recorded for cane diameter. The effect of genotype and crop interaction was highly significant fornumber of tillers while non-significant for other characters. Broad sense heritability for different characters showed varying levels and it was higher for internodes length (43%), number of nodes (39%),cane length (39%), plant height (30%), number of tillers (12%), cane yield (41%) andnumber of millablecane (35%),respectively indicating that these traits could be selected in the improvement of crop yield. Genetic gain was higher for cane length (36.53 cm), plant height (31.84 cm) and number of tillers (12.98 tillers per 9 m2). Number of tillers (rg=0.96, rp =0.83), plant height (rg =0.95, rp = 0.81), cane length (rg =0.90, rp = 0.76), number of nodes (rg =0.79, rp = 0.67), internodes length (rg =0.80, rp =0.74) and number of millablecane (rg =0.96, rp = 0.87) exhibited highly significant and positive correlation with cane yield at genotypic and phenotypic levels.

Received | March 29, 2017; Accepted | October 23, 2017; Published | November 18, 2017

*Correspondence | Amjad Ali, Sugar Crops Research Institute Mardan, Pakistan; Email: [email protected]

Citation | Ali, A., S.A. Khan, A. Farid, A. khan, S.M. Khan and N. Ali.2017. Assessment of sugarcane genotypes for cane yield. Sarhad Journal of Agriculture, 33(4): 668-673.

DOI | http://dx.doi.org/10.17582/journal.sja/2017/33.4.668.673

Keywords | Sugarcane, Heritability, Correlation, Selection, Cane yield

Introduction

Sugarcane (Saccharum officinarum L.) is an important cash crop (Falcon, 1964). Sugarcane varieties tend to run out or decline after some years in a specific area (Khan et al., 2009). To obtain high yield on sustainable basis, it has been essential to substitute varieties regularly with new clones. The sugarcane varieties is clonally propagated and is not expected to undergo genetic change as occur in a seed propagated crop though the variety decline occurs due to disease incidence and thus need to be replaced (Poehlman,1959). In Pakistan due to low yield condition it has been necessary to develop sugarcane varieties with high cane and sugar yield (Baloch et al., 2002; Arain et al., 2011). It is important that the germplasm must contain sufficient amount of variations for the varietal development program (Chaudhary, 2001). Like other vegetatively propagated crops introduction and clonal selection have been the principal breeding procedures and are playing an important role in varietal development program at SCRI Mardan, Khyber Pakhtunkhwa (Tahir et al., 2013). In any breeding population, there are genetic alterations present and these variations are the basic mechanisms to develop selection and breeding approaches that eventually lead to better genetic gains. In the selection procedure, identification and separation of the dependent and independent characters are important. For a successful selection program developmental criterion is mandatory. Genotypes with better yield and quality parameters and broader adoptability can be selected and suggested for farming. Repeatability (broad sense heritability) and genetic gains study can be largely used for a good breeding programs study (Chaudhary, 2001). The characters with higher repeatability and genetic gain can be used for selection. The correlations among various characters with yield and their mutual correlation study is important and also gives an insight between the parameters relation (Kang et al., 1983).

Based on the above facts, the present research study was designed to evaluate the genotypes through repeatability, estimated genetic gain and path coefficient analysis. This will help understand the importance of genetic potential of various growth, cane, and yield traits. It will help in developing a selection criterion for sugarcane breeding programs in Khyber Pakhtunkhwa, Pakistan.

Materials and Methods

The study was conducted at the SCRI, Mardan, located at 34o North latitude and 72o East longitude, altitude 283 meter, total rainfall 696mm (summer 488mm, winter 208mm), summer mean temperature 39.8oC , winter mean temperature 1.33oC with a mean relative humidity of 60.8% Pakistan onsugarcane plant crop during 2012-13 and 2013-14. Materials comprised 14 sugarcane genotypes and two check cultivars (Table 1) arranged in a randomized complete block design with three replications. These genotypes were advanced from previous selection stages with diverse origins. A plot for each genotype was 10 m long and 6.7m wide, having 7 rows (150 buds per row) with a row-to-row distance of 90 cm. Recommended dose of fertilizer i.e. N, P2O5 and K2O at rates of 150: 100:100 was given to the crop. Data were recorded, on the following growth, cane and yield charactersof sugarcane.

Growth traits

Data on number of tillers was recorded by counting numbers of tillers in the central row having 10 meter length in each plot.

The data on plant height wererecorded on five randomly selected plants with the help of a tap in centimeters and then were averaged.

Table 1: List of sugarcane genotypes and their sources.

S. No.	Genotype	Source
1.	MS-2000-Ho-535	Houma , Louisiana, USA
2.	MS-99-Ho-6	Houma , Louisiana, USA
3.	MS-2000-Ho-115	Houma , Louisiana, USA
4.	MS-2000-Ho-357	Houma , Louisiana, USA
5.	S-98-SSG-363	Guatemala
6.	S-98-SSG-612	Guatemala
7.	MS-91-CP-248	Canal Point, USA
8.	MS-91-CP-249	Canal Point, USA
9.	S-92-US-72	Canal Point, USA
10.	MS-91-CP-523	Canal Point, USA
11.	MS-92-CP-99	Canal Point, USA
12.	MS-2000-Ho-360	Houma , Louisiana, USA
13.	MS-2003-HS-274	Habib Sugar Mill Research Farm, Sindh, Pakistan
14.	MS-2003-HS-366	Habib Sugar Mill Research Farm, Sindh, Pakistan
15.	CP-77/400	Check cultivar
16.	Mardan-93	Check cultivar

Cane traits

Five plants were selected at randomin each genotype. The trash was removed and the length in centimeters was measured in the month of October and the average lengths were noted.

Number of nodes was recorded by counting nodes in5 plants taken in random for each genotype and were averaged.

Data on internode length was determined by measuring internodes length of the 5th internode from the baseof five randomly selected stalks and then averaged.

Cane diameter of the stalk was measured usingdigital Vernier Caliper (0-150mm digital caliper. Stainless Hardened. Germany)for each of the 5 randomly selected stalk at the 5th internode from the base.

Yield traits

Number of millablecane was taken by counting the number of millablecane in the mid row (without the undeveloped tillers).

Table 3: Basic statistics of 16 sugarcane genotypes evaluated as plant crops at SCRI, Mardan.

Traits	Mean	δ2	δ	Minimum	Maximum	δ2 as % of mean
Number of Tillers	222.70	805.50	28.38	163.33	287.50	361.70
Plant Height (cm)	200.98	722.82	26.89	164.17	273.33	359.65
Cane length (cm)	163.50	656.77	25.63	122.00	240.00	401.69
Number of nodes	13.09	3.51	1.87	9.50	18.00	26.79
Internodes length (cm)	14.69	1.64	1.28	12.28	16.57	11.19
Cane diameter (cm)	0.94	0.00	0.05	0.83	1.03	0.30
Number of millablecane	78.45	41.39	6.43	65.67	94.67	52.76
Cane yield (tha-1)	77.39	17.13	4.14	70.33	88.33	22.13

Data on cane yield was taken by weighing the cane without trash per plot in kilograms and converted in to tonsha-1 by using the following formula.

Where;

x: yield in kg per plot (Tahir et al., 2014a).

Heritability was worked using the mean squares(Singh et al., 1993).Genetic advance was estimated for important traits using 10% selection intensity (Johnson et al., 1955):

Genetic gain= K ×σp× h2

Where;

K: 1.75 at 10% selection intensity; σp: Phenotypic standard deviation; h2: Heritability.

PLABSTAT version 3A (Utz, 2011) was used to determine correlations at phenotypic and genotypic levels. Standard errors for both correlations were calculated as defined by Mode and Robinson (1959).

Results and Discussion

Statistical analysis

Mean squares pertaining to crops were highly significant for number of tillers, significant for number of millablecane and cane yield while non-significant for the remaining characters (Table 2). Similarly, among genotypes highly significant differences were present for number of tillers, plant height, cane length, number of nodes, internodes length, number of millablecane and cane yield while non-significant for cane diameter. The effect of genotype and crop interaction was found non-significant for all parameters except number of tillers.

Basic statistics of all the parameters

The number of tillersper row ranged from 163.33to 287.50with mean value of 222.69. Plant height ranged from 164.16 cm to 273.33 cm with a mean value of 200.98 cm, (Table 3). Cane length ranged from 122 cm to 240 cm with mean value of 163.5 cm. Number of nodes ranged from 9.5 to 18 with mean value of 13.09. Internodes length ranged from 12.28 cm to 16.56 cm with mean value of 14.69 cm. Cane diameter ranged from 0.83 cm to 1.03 cm with mean value of 0.93 cm. The number of millablecane ranged from 65.66 to 94.66 with mean value of 78.45 while cane yield ranged from 70.33 to 88.33 9 t ha-1 with mean of 77.38 (t ha-1). Higher variance and standard deviation was shown by tillering (805.50 and 28.38), plant height (722.82 and 26.89) and cane length (656.77 and 25.63). Almost similar trend was found in these characters as means of percentof variance.

Table 4: Variances, heritability and expected genetic gain for growth, cane and yield traits of sugarcane genotypes.

Traits	Vg	Vgxc	Ve	h2	Exp. GG
Number of tillers	237.56	858.86	831	0.12	12.98
Plant height (cm)	580.31	-238.45	1570.37	0.30	31.84
Cane length (cm)	589.95	-255.72	1168.03	0.39	36.53
Number of nodes	2.90	-0.456	5.00	0.39	2.55
Internodes length (cm)	1.38	-0.131	1.94	0.43	1.86
Cane diameter (cm)	0.0017	-0.0017	0.0115	0.15	0.04
Number of millablecane	28.84	10.47	43.85	0.35	7.59
Cane yield (t ha-1)	13.798	-0.022	20.052	0.41	5.69

Table 5: Phenotypic (above diagonal) and genotypic (below diagonal) correlations among growth, cane and yield characters of sugarcane genotypes.

	Number of tillers	Plant height	Cane length	Number of nodes	Internodes length	Cane diameter	Number of millablecane	Cane yield
Number of Tillers	1	0.77**	0.74**	0.68**	0.81**	0.29	0.86**	0.83**
Plant height	0.93++	1	0.93**	0.78**	0.80**	0.44	0.84**	0.81**
Cane length	0.86++	0.99++	1	0.85**	0.72**	0.27	0.79**	0.76**
Number of nodes	0.81++	0.91++	0.97++	1	0.62**	0.43	0.67**	0.67**
Intern-odes length	0.93++	0.92++	0.79++	0.69++	1	0.51*	0.78**	0.74**
Cane diameter	0.41	0.60+	0.23	0.56+	0.75+	1	0.41	0.26
Number of millable-cane	0.98++	0.99++	0.92++	0.74++	0.89++	0.62+	1	0.87**
Cane yield	0.96++	0.95++	0.90++	0.79++	0.80++	0.39	0.96++	1

Repeatability (Broad Sense Heritability) and genetic gain

Heritability showed the effectiveness of selection, the breeding method adopted and response of various traits under a particular selection pressure (Jamoza et al., 2014). Genetic gain is the measure which predicts gain from selection. Higher genetic gain results in higher probability of selecting individuals with better performance (Tahir et al., 2014 b).The values of the environmental variances were larger than genetic variances for almost all the parameters studied (Table 4). High broad sense heritability was recorded for internodes length (43%), cane yield (41%), number of nodes (39%), cane length (39%) and number of millablecane (35%). Higher genetic advances were noted for cane length (36.53 cm), plant height (31.84 cm) and number of tillers (12.98 tillers per 9 m2).

Correlation

All parameters except quality traits showed positive and highly significant correlation both at phenotypic and genotypic levels with cane yield (Table 5). The highest phenotypic correlation was shown by number of millablecane (0.87), number of tillers (0.83) and plant height (0.81) while the highest genotypic correlation was shown by number of tillers (0.96), number of millablecane (0.96), plant height (0.95) and cane length (0.90), respectively. Cane diameter was positively associated with cane yield at both the levels and its association was lower than other cane parameters.

The significant genotypic effects showed that there are sufficient variability among the genotypes and the possibility of genetic improvement (Punia, 1982; Khan et al., 2004). The relatively large genotypic mean squares showed that clones have a broader background and differed in their potential for the individualities. Significant crops interactions for number of tillers, number of millablecane and cane yield showed that mean performances of the genotypes were influenced by 1st and 2nd plant crop i.e. cropping year. Across the years relative ranking of the genotypes changes which resulted significant crops interaction. The genotype and yearswere not significant for all the traits except tilleringshowing that the mean performance of the genotypes was similar over the year suggesting locational trials rather than the years (Chang, 1996).

A varietal improvement program depends on the genetic variability present in a population.The amount of genetic variation for a trait present in a genotype can beefficiently estimated by heritability (Chaudhary, 2001). High broad sense heritability estimates were obtained for internodes length (43%), cane yield (41%), cane length (39%), number of nodes (39%) and number of millablecane (35%) (Table 4).It is evident that some proportion of the total variance is heritable and these traits must be given importance during selection. The study showed thatinternodes length, cane length and number of nodes were reliable selection parameters.

Both heritability and genetic gain made the selection process effective (Shoba et al., 2009). The high genetic gain observed for cane length (36.53 cm) followed by plant height (31.84 cm) and number of tillers per 9m2 (12.84) was the result of high broad sense heritability for these traits (Bakshi, 2005). These results suggest that considerable scope for improvement in these traits is present. High genetic advance has also been reported for single stalk weight and number of millablecane by Tyagi and Singh, (1998), Kamat and Singh (2001). Gravois and Milligan (1992) found that single stalk weight and number of millablecane gave larger value for broad sense heritability suggesting that these traits are under the control of additive genetic effects.

The strong genetic correlation between agronomic traits suggests that selection of these traits could simultaneously improve these traits. Sanghera et al. (2015) found positive phenotypic and genotypic correlation of different morphological and cane characters with cane yield. Cane length and weight, number of nodes and internodes length were positively and substantially correlated with cane yield as reported by Chaudhary et al. (2003).

Conclusion

This study revealed genetic and phenotypic association of cane yieldwith different parameters. Due to diverse background sufficient variability were present among the genotypes. The results suggest that assessment of sugarcane genotypes for cane yield in the plant crop in many years should identify superior clones. This testing approach coupled with a selection strategy based on internodes length, number of nodes, cane length, number of millablecane and plant height might result in significant genetic improvement in cane yield on the basis of higher heritability and genetic gain.

Authors Contribution

The manuscript was the part of PhD thesis of Amjad Ali and Sher Aslam Khan supervise him. Abid Farid critically reviewed the first draft. Ayub Khan helped in compilation of results. Shah Masud Khan helped in relevant literature search. Naushad Ali helped during fieldwork.

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