Antimicrobial and Phytochemical Evaluation of Ziziphus mauritiana var. spontanea Edgew. and Oenothera biennis L.
Antimicrobial and Phytochemical Evaluation of Ziziphus mauritiana var. spontanea Edgew. and Oenothera biennis L.
Ambrin1*, Ghulam Dastagir1, Jehan Bakht2 and Muhammad Adil1
ABSTRACT
The present study investigates antimicrobial activities of different solvent extracted samples of Ziziphus mauritiana var. spontanea and Oenothera biennis against different microbes. Ethyl acetate and ethanolic extracts of Z. mauritiana var. spontanea measured the highest zone of inhibition against Staphylococcus epidermidis, Escherichia coli and Salmonella typhi. The MIC values of ethyl acetate and ethanolic extracts of Z. mauritiana var. spontanea were the lowest for Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Shigella flexneri and Staphylococcus aureus. Highest zone of inhibition against Klebsiella pneumonia and Pseudomonas aeruginosa was shown by ethyl acetate and ethanolic extracts of O. biennis. Lowest MIC value was noted for ethyl acetate and ethanolic extracts of O. biennis against Staphylococcus epidermidis, Staphylococcus aureus, Salmonella typhi and Shigella flexneri. The ethyl acetate and ethanolic extracts of Z. mauritiana var. spontanea showed good antifungal activity against Aspergillus niger, Penicillium notatum. Minimum MIC value was shown by ethyl acetate and ethanolic extracts of Z. mauritiana against Aspergillus fumigatus and Aspergillus niger. The ethyl acetate and ethanolic extract of O. biennis revealed maximum antifungal activity against Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus. The MIC value of ethyl acetate and ethanolic extracts of O. biennis was lowest for Fusarium solani and Penicillium notatum. Phytochemical screening of Z. mauritiana var. spontanea and O. biennis revealed the presence of amino acids, reducing sugar, proteins, triterpenoids, gums and mucilages, fats, steroids, phenols and phytosterol.
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