Submit or Track your Manuscript LOG-IN

A New Species of the Genus Pseudostigmaeus Wood (Acari: Prostigmata: Stigmaeidae) from Pakistan

PJAR_33_2_311-315
                                         
               
 

 

 

 

Research Article

A New Species of the Genus Pseudostigmaeus Wood (Acari: Prostigmata: Stigmaeidae) from Pakistan

Bilal Saeed Khan1*, Muhammad Afzal2, Muhammad Asif Qayyoum1, Imran Ali3 and Abdul Ghaffar3

1Department of Entomology, University of Agriculture, Faisalabad, Pakistan; 2Professor and Dean, College of Agriculture, University of Sargodha, Sargodha, Pakistan; 3Ayub Agricultural Research Institute, Faisalabad, Pakistan.

Abstract | Mites of the family Stigmaeidae are well known as predators against phytophagous mites, lepidopterous eggs and bodies of small soft insects. A random survey was conducted to explore the predatory mite fauna from Punjab, Pakistan. The holotype female (male unknown) of the new mite species Pseudostigmaeus sorghumus sp. nov was collected from city Dera Ghazi Khan on Sorghum bicolor (L.) and described. Fifteen paratypes were also collected from same collection data and seven were from Muzaffargarh.


Received | November 24, 2019; Accepted | January 30, 2020; Published | June 15, 2020

*Correspondence | Bilal Saeed Khan, Department of Entomology, University of Agriculture, Faisalabad, Pakistan; Email: bilalentomologyuaf@gmail.com; dr.bilal.saeed@uaf.edu.pk

Citation | Khan, B.S., M. Afzal, M.A. Qayyoum, I. Ali and A. Ghaffar. 2020. A new species of the genus Pseudostigmaeus wood (Acari: Prostigmata: Stigmaeidae) from Pakistan. Pakistan Journal of Agricultural Research, 33(2): 311-315.

DOI | http://dx.doi.org/10.17582/journal.pjar/2020/33.2.311.315

Keywords | Stigmaeidae, Pseudostigmaeus, Acari, New species, Predatory mite



Introduction

Stigmaeidae is considered as major family within Raphignathoidea and comprise a large cosmopolitan of near about 500 species grouped into 32 valid genera (Dogaan et al., 2011; Khanjaani et al., 2012a) including the genus Ueckermann in 2008. Stigmaeid mites naturally minute, often red to yellow orange arachnids, that occur in many habitat and giving an important slice of the soil texture, leaf litter and aerial plant parts. Stigmaeids are proved to be a large cluster of predators that feed on variety of arthropod pest, ectoparasites of flies and also pollen feeders (Summers, 1966; Meyer and Ueckerman, 1987; Walter et al., 2009). Stigmaeid mites are considered as efficient feeders against the tetranychid, tenuipalpid and eriophyid mites (Santos and Laing, 1985). Stigmaeid mites are well known predators against the phytophagous mites, soft small insect bodies and feeding on a variety of arthropods (Swift, 1987; Akyol and Kocc, 2007; Khanjani et al., 2010). The total eight species of genus Pseudostigmaeus were introduced from world (Pseudostigmaeus wood as type specimen) including one from Pakistan i.e., Pseudostigmaeus jhangensis. All specimens were deposited in the Acarology Research Laboratory, Department of Entomology, University of Agriculture, Faisalabad, Pakistan.

Diagnosis

Wood (1967) created the genus Pseudostigmaeus, distinguishing it by the presence of 3 pairs of setae on median propodosomal plate, the presence of inter coxal plates and 1-2 setae on coxa II. Body soft and weakly reticulated, chelicerae separated, Palptibia with 2 simple setae, main claw sometimes with 1 accessory claw, palptarsus bearing 4 setae, 1 soledion, 1 spine and 1 trifid eupathidium. Dorsal setae 11-13 pairs (sometimes vi absent as in this new species). Dorsum covered by 1 propodosomal shield and 1 pair of small suranal shield. Propodosoma 1 pair of eyes. Mostly genital area provided with a single genital pore, 3 pairs of anogenital setae and 3 pairs of paraproctal setae. Mites of this genus often collected from living plants or soil leaf litter. The mites of this genus were recorded from New Zealand, China, Ireland, South Africa and Pakistan, respectively. A new species has been collected by the author from Punjab, Pakistan and described here in this manuscript which is compared with Pseudostigmaeus capensis Meyer and P. ueckermanni Ueckermann for differences.

Materials and Methods

Mite of the genus Pseudistigmaeus (Acari: Prostigmata: Stigmaeidae) were collected by (Bilal Saeed Khan) from Sorghum bicolor (L.) through sieve collection method. The slides were equipped by using the solution of Hoyer’s and drawn with the help of eye piece /microscope. The species have been identified with the help of the accessible keys and text. Grandjean’s system of terminology (Grandjean, 1944) with modifications/additions made by (Summers, 1960; Gonzalez, 1965) were used. All measurements are in micrometers (µm). The abbreviations used in this manuscript as:

ve (be): pre-ocular dorsal setae; sci (ce): post ocular dorsal setae; c1 (a): dorsocentral dorsal setae (l); d1 (b): dorsocentral dorsal setae (lI); e1 (c): mediozonal setae; h1 (e): central suranal setae; c2 (he): humeral setae; d2 (la): dorsolateral dorsal setae; f1 (Ii): intercalary dorsal setae; e2 (Im): lateral zonal setae; ag1, ag2, ag3: agenital setae; ps1, ps2, ps3: paraproctal setae; he: humeral seta (ventrally visible); vi (ae): vertical dorsal setae (absent in this new species). Notes: i. Kethley (1990) has given dorsal setal nomenclature and shield designations respectively. ii. Oldsetal abbreviations are given in brackets.

Results and Discussion

Wood 1967 erected the genus Pseudostigmaeus based on type species as P. collyerae Wood and P. striatus Wood at the same time. Meyer and Ryke (1969) described P. capensis Meyer from South Africa and redefined the genus with a new diagnosis. But later, P. capensis removed and designated as the genotype of a new genus Parastigmaeus Kuznetsov (Kuznetsov and Petrov, 1984). Wood (1970) described 1 species (Pseudostigmaeus longisetis) Campell Island from moss. Later Wood provided a key to species from New Zealand in 1971. Momen (1987) described Pseudostigmaeus arboricolus from apple tree in Ireland. A new species P. ueckermanni (1987) was collected from China.

Description of Pseudostigmaeus sorghumus sp. nov

Female: (Dorsum) Body long slender pear shape 435 µm (without gnathosoma), 230 µm wide, chelicerae length 92 µm, stylets 42 µm long. Palptarsus slightely longer than main claw. palptarsus well developed and reaches past the tibial claw. The setae disposed on the palpal segment provided femur 2, genu 2, tibia 2, tarsus with 4 minute setae and provided with trifid sensillum. Base of the chelicerae are not fused together. Peritreme obvious having the length of 73 µm, eyes 1 pair large in size (Figure 1). Post-ocular body absent. Propodosomal shield having rounded corners. The 1st pair of propodosomal seta ve is not located on anterior margin of propodosomal plate. The 2nd pair of propodosomal seta sci situated immediately in front of eyes. The 3rd pair of propodosomal seta sce shorter in length than the seta sci and not present on the propodosomal plate. Humeral seta he absent dorsally. Metapodal shield completely invisible and whole area covered with straight and vertical striations. Dorsal idiosomal setae provided with tiny platelets except f1; seta f1 present. 5 pairs of setae on hysterosoma shorter in length than the setae h1 and h2. The respective distances between ve-ve 33, sci-sci 68, sce-sce 145, c1-c1 85, d1-d1 93, e1-e1 50, f1-f1 85, c1-d1 82, d1-e1 60, e1-f1 37, d2-d2 180, e2-e2 155. The length of dorsal setae ve 17, sci 23, sce 15, he 17; seta h1 16, d1 15, e1 15, d2 15, e2 13, f1 18, h2 20, h1 28 and f118.


Venter: Ventral gnathosoma provided 3 pairs of setae, 3rd pair of seta longest. In 5 pairs of idiosomal setae, 1st pair much longer than others and this 1st pair present between coxae I and II. Humeral seta he present ventrally. 3 pairs of anogenital setae (ag1, ag2, ag3) smooth while regarding paraproctal setae, the 2nd and 3rd one is barbed except Ist pair (Figure 2).


Legs: Chaetotaxy of legs I-IV as (Figure 3): coxae 2-1-1-1; trochanters 0-0-1-1; femora 4-4-2-2; genua 3-1-0-0; tibiae 3-4-3-4 and tarsi 10-6-5-5.


Etymology: This new species refers to the crop name from where it was collected.

Adult male: Unknown.

Type: Holotype, female collected from Dera Ghazi Khan on Sorghum bicolor by (Bilal Saeed Khan). 15 paratype females, 8 same collection data and 7 from date palm in Muzaffargarh. All specimens were deposited in Acarology Research Laboratory, Department of Entomology, University of Agriculture, Faisalabad.

Remarks: This new species closely related with P. capensis but separated it due to following points:

  1. 1. Postocular body present in P. capensis while absent in this sp. nov.
  2. 2. 1st pair of seta ve is not located on anterior margin of propodosomal plate in this sp. nov. while ve present very anteriorly in P. capensis.
  3. 3. sce shorter than seta sci in this n.sp. but sce longer in P. capensis.
  4. 4. Humeral seta he absent dorsally in this n.sp. while present in Pseudostigmaeus capensis.
  5. 5. f1 present in this n.sp. while absent in P. capensis.
  6. 6. 3 pairs of anogenital setae in this n.sp. while 4 in P. capensis.

Note: Dendrogram of 5 species of the genus P. from Punjab, Pakistan is also given in manuscript.

Key to genus Pseudostigmaeus form Punjab, Pakistan (Females)

  1. 1. Tridentate spine absent on tarsalpeg; palptibia with 1 seta; palp tarsalpeg equal with main claw; dotted striations on propodosomal shield; 11-pairs dorsal setae..…. jhangensis
  2. 2. Tridentate spine present; palptibia with more than 1 seta; palp tarsalpeg longer than main claw; propodosomal shield without dotted striations; 12-13 pairs dorsal setae…… 2
  3. 3. Propodosomal shield incompletely visible; metapodal shield visible; ventral idiosoma partially striated; anogenital setae serrate……solanumus (n.sp) (Figures 1 and 2)
  4. 4. Propodosomal shield completely visible; metapodal shield not visible; ventral idiosoma completely striated; anogenital setae not serrate………3
  5. 5. Dorsal setae smooth; humeral seta ventrally present; anogenital plate not rounded in shape; smooth paragenital setae; barbed anogenital setae; 3 setae on tibia-I ..……sorghumus sp.nov.
  6. 6. Dorsal setae not smooth; humeral seta ventrally absent; anogenital plate rounded in shape; paragenital setae not smooth; anogenital setae not barbed; more than 3 setae on tibia-I…….4
  7. 7. Palpgenu with 2 setae; postocular body absent; seta f1 present; 4 pairs of anogenital setae; ventral idiosoma provided with 5 setae…...ueckermanni Fan and Liu
  8. 8. Palpgenu without setae; postocular body present; seta f1 absent; 3 pairs of anogenital setae; ventral idiosoma provided with 6 setae………capensis (Meyer)

 


Acknowledgement

Authors acknowledge Higher Education Commission of Pakistan for financial support to conduct this research work. We are thankful to Dr. Eddi Ueckerman from South Africa for literature help and also grateful for the guidance of Prof. Dr. Shamshad Akbar (Late) regarding preparation of manuscript. This paper is also presented by author # 1 during European Congress of Entomology Napoli, Italy 2018.

Author’s Contribution

Bilal Saeed Khan: Principal and Corresponding author. Wrote the abstract and introduction. Did methodology of work ad data collection.

Muhammad Afzal: P.I of whole research work project. Plan the idea of research work.

Muhamma Asif Qayyoum: Development of key formation and character data entry. Technical inputs.

Imran Ali: Write up of key characters and analysis and improve discussions.

Abdul Ghaffar: Revised study if genus and literature help with results/conclusion.

References

Akyol, M. and K. Koçc. 2007. Four new species of the genus Stigmaeus (Acari, Stigmaeidae) from Turkey. Arch. des Sci. 60: 41–50.

Dogan, S., G. Donel and S. Ozcelik. 2011. A new eyeless mite species of the genus Eustigmaeus Berlese (Acari: Stigmaeidae) from Turkey. Turk. J. Zool. 35(2): 175-181.

Gonzalez, R.H., 1965. A taxonomic study of the genera Mediolata, Zetzellia and Agistemus (Acari: Stigmaeidae). Univ. Calif. Publ. Ent., 41: 1‒64.

Grandjean, F., 1944. Observations sur les acariens de la famille des stigmaeidae. Arch. Sci. Phys. Nat., 26: 103-131.

Kethley, J., 1990. Acarina: Prostigmata (Actinedida). In: soil biology guide. In: Dindal DL, editor. USA: John Wiley and Sons: pp. 1376.

Khanjani, M., H. Raisii, H. Izadi and E.A. Ueckermann. 2010. A new species of genus Chelostigmaeus Willmann (Acari: Stigmaeidae) from eastern Iran. Int. J. Acarol., 36(1): 7-13. https://doi.org/10.1080/01647950903473323

Khanjani, M., B.A.A. Fayaz and S. Dogan. 2012a. A new species of the genus Prostigmaeus Kuznetzov (Acari: Stigmaeidae) from western Iran. North-western J. Zool., 8(1): 27-30.

Kuznetzov, N.N. and V.M. Petrov. 1984. Predacious mites of the blatic republics (Parasitiformes: Phytoseiidae, Acariformes: Prostigmata). Zinatne, Riga, USSR, pp.144.

Meyer, M.K.P. and E.A. Ueckermann. 1987. Afrotropical stigmaeidae (Acari: Prostigmata). Phytophylactica. 19: 371-397.

Meyer, M.K.P. and P.A.J. Ryke. 1969. Some stigmaeid mites from South Africa (Acari: Trombidiformes). Acarologia.11(2): 207‒217.

Momen, F.M., 1987. Three new species of stigmaeid mites (Acari; Prostigmata) from Ireland. Z. Angew. Zool. 74(2): 223‒229.

Santos, M.A. and J.E. Laing. 1985. Stigmaeid predators. In: Helle W. and Sabelis M.W. (Eds) Spider Mites: Their Biology, Natural Enemies, and Control. Vol. 1B. Elsevier, Amsterdam, Netherlands: 197–203.

Summers, F.M., 1966. Genera of the mite family Stigmaeidae Oudemans (Acari). Acarologia, 8(2): 230-250.

Summers, F.M., 1960. Several stigmaeid mites formerly included in Mediolatare described in Zetzellia Oudemans and Agistemus, 1960. New genus. Proc. Ent. Soc. Wash. 62(4): 233‒247.

Swift, S.F., 1987. A new species of Stigmaeus (Acari: Prostigmata: Stigmaeidae) parasitic on phlebotomine flies (Diptera: Psychodidae), Int. J. Acrol., 13(4): 239-243. https://doi.org/10.1080/01647958708683778

Walter, D.E., E.E. Lindquist, I.M. Smith, D.R. Cook and G.W. Krantz. 2009. Order Trombidiformes. In: Krantz, G.W. and Walter, D.E. (Eds.) A Manual of Acarology. 3rd ed. Lubbock: Texas Tech Univ. Press, pp. 5–53.

Wood, T.G., 1967. New Zealand mites of the family Stigmaeidae (Acari: Prostigmata). Trans. R. Soc. N. Z. Zool., 9(9): 93‒139.

Wood, T.G., 1970. Stigmaeidae (Acari: Prostigmata) from Campbell Island. Acarologia. 12(4): 677‒ 683.

Wood, T.G., 1971. New species and records of stigmaeidae (Acari: Prostigmata) from New Zealand. ID. Genus Stigmaeus Koch. N.Z. J. Sci., 14(1): 54‒61.

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Agricultural Research

June

Vol. 33, Iss. 2, Pages 192-421

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe