ResearchersLinks
Hosts and VirusesA ResearchersLinks journal
Submit manuscriptReview for this journal

Mini-Review · Volume 2, Issue 3, 9 June 2015 · Open Access

RT-NASBA: An Ideal Approach for the Detection of Diarrhoea-Related Viruses

· ·

Affiliations & Notes ⌄
  1. 1Key Laboratory of Diarrhea Disease Detection, Zhuhai International Travel Healthcare Center, Zhuhai Entry-Exit Inspection and Quarantine Bureau, Zhuhai, Guangdong, China. E-mail | zhithc-lab@hotmail.com
Article Info ⌄
Journal:
Hosts and Viruses
Published:
9 June 2015
Download PDF ePub eFLIP Set Alert Get Rights Reprints

From the article

  • Abstract | RT-NASBA is a rapid and sensitive diagnostic system, which combines nucleic acid sequence-based amplification (NASBA) and molecular beacon technique.
  • Clinical evaluation indicated that the assay was 100% concordant to multiplex RT-PCR and was reliable for the detection of both single infection and multiple infections in real stool samples.
  • Real-time molecular beacon NASBA for rapid and sensitive detection of norovirus GII in clinical samples.
  • A nucleic acid sequence-based amplification assay for real-time detection of norovirus genogroup II.

Selected from the article's own text.

Abstract

RT-NASBA is a rapid and sensitive diagnostic system, which combines nucleic acid sequence-based amplification (NASBA) and molecular beacon technique. Recently there have been two major progresses in the field, in which RT-NASBA technique was applied for the detection of diarrhoea related viruses. This mini review overviewed the basic principle of RT-NASBA and discussed the significance of these new findings.

Acknowledgement

This work was supported by the Science and Technology Grant from Zhuhai, China (grant#PC20081002) and special grant from Zhuhai International Travel Healthcare Center. No conflict of interest exists.

Figures

Figure 1
Figure 1.

Principle of RT-NASBA assay

References

  1. Atmar, R.L., Estes, M.K., 2001. Diagnosis of noncultivatable gastroenteritis viruses, the human caliciviruses. Clinical microbiology reviews 14, 15-37. http://dx.doi.org/10.1128/CMR.14.1.15-37.2001[CrossRef]
  2. Churruca, E., Girbau, C., Martinez, I., Mateo, E., Alonso, R., Fernandez-Astorga, A., 2007. Detection of Campylobacter jejuni and Campylobacter coli in chicken meat samples by real-time nucleic acid sequence-based amplification with molecular beacons. International journal of food microbiology 117, 85-90. http://dx.doi.org/10.1016/j.ijfoodmicro.2007.02.007[CrossRef]
  3. Compton, J., 1991. Nucleic acid sequence-based amplification. Nature 350, 91-92. http://dx.doi.org/10.1038/350091a0[CrossRef]
  4. Costa, A.M., Lamb, D., Garland, S.M., Tabrizi, S.N., 2008. Evaluation of LightCycler as a platform for nucleic acid sequence-based amplification (NASBA) in real-time detection of enteroviruses. Current microbiology 56, 80-83. http://dx.doi.org/10.1007/s00284-007-9043-2[CrossRef]
  5. Farkas, T., Jiang, X., Guerrero, M.L., Zhong, W., Wilton, N., Berke, T., Matson, D.O., Pickering, L.K., Ruiz-Palacios, G., 2000. Prevalence and genetic diversity of human caliciviruses (HuCVs) in Mexican children. Journal of medical virology 62, 217-223. http://dx.doi.org/10.1002/1096-9071(200010)62:2<217::AID-JMV13>3.3.CO;2-6[CrossRef]
  6. Forbi, J.C., Gabadi, S., Iperepolu, H.O., Esona, M.D., Agwale, S.M., 2010. Quantification of human immunodeficiency virus-1 viral load using nucleic acid sequence-based amplification (NASBA) in north central Nigeria. Nigerian journal of clinical practice 13, 284-287.
  7. Fukuda, S., Sasaki, Y., Seno, M., 2008. Rapid and sensitive detection of norovirus genomes in oysters by a two-step isothermal amplification assay system combining nucleic acid sequence-based amplification and reverse transcription-loop-mediated isothermal amplification assays. Applied and environmental microbiology 74, 3912-3914. http://dx.doi.org/10.1128/AEM.00127-08[CrossRef]
  8. Guatelli, J.C., Whitfield, K.M., Kwoh, D.Y., Barringer, K.J., Richman, D.D., Gingeras, T.R., 1990. Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proceedings of the National Academy of Sciences of the United States of America 87, 1874-1878. http://dx.doi.org/10.1073/pnas.87.5.1874[CrossRef]
  9. He, X.Q., Cheng, L., Zhang, D.Y., Xie, X.M., Wang, D.H., Wang, Z., 2011. One-year monthly survey of rotavirus, astrovirus and norovirus in three sewage treatment plants in Beijing, China and associated health risk assessment. Water science and technology : a journal of the International Association on Water Pollution Research 63, 191-198.
  10. Jean, J., Blais, B., Darveau, A., Fliss, I., 2002. Rapid detection of human rotavirus using colorimetric nucleic acid sequence-based amplification (NASBA)-enzyme-linked immunosorbent assay in sewage treatment effluent. FEMS microbiology letters 210, 143-147. http://dx.doi.org/10.1016/S0378-1097(02)00614-6[CrossRef]
  11. Koh, H., Baek, S.Y., Shin, J.I., Chung, K.S., Jee, Y.M., 2008. Coinfection of viral agents in Korean children with acute watery diarrhea. Journal of Korean medical science 23, 937-940. http://dx.doi.org/10.3346/jkms.2008.23.6.937[CrossRef]
  12. Lamhoujeb, S., Charest, H., Fliss, I., Ngazoa, S., Jean, J., 2009. Real-time molecular beacon NASBA for rapid and sensitive detection of norovirus GII in clinical samples. Canadian journal of microbiology 55, 1375-1380. http://dx.doi.org/10.1139/W09-105[CrossRef]
  13. Lau, L.T., Feng, X.Y., Lam, T.Y., Hui, H.K., Yu, A.C., 2010. Development of multiplex nucleic acid sequence-based amplification for detection of human respiratory tract viruses. Journal of virological methods 168, 251-254. http://dx.doi.org/10.1016/j.jviromet.2010.04.027[CrossRef]
  14. Le Guyader, F.S., Le Saux, J.C., Ambert-Balay, K., Krol, J., Serais, O., Parnaudeau, S., Giraudon, H., Delmas, G., Pommepuy, M., Pothier, P., Atmar, R.L., 2008. Aichi virus, norovirus, astrovirus, enterovirus, and rotavirus involved in clinical cases from a French oyster-related gastroenteritis outbreak. Journal of clinical microbiology 46, 4011-4017. http://dx.doi.org/10.1128/JCM.01044-08[CrossRef]
  15. Lindell, A.T., Grillner, L., Svensson, L., Wirgart, B.Z., 2005. Molecular epidemiology of norovirus infections in Stockholm, Sweden, during the years 2000 to 2003: association of the GGIIb genetic cluster with infection in children. Journal of clinical microbiology 43, 1086-1092. http://dx.doi.org/10.1128/JCM.43.3.1086-1092.2005[CrossRef]
  16. Mo, Q.H., Wang, H.B., Dai, H.R., Lin, J.C., Tan, H., Wang, Q., Yang, Z., 2015a. Rapid and simultaneous detection of three major diarrhea-causing viruses by multiplex real-time nucleic acid sequence-based amplification. Archives of virology 160, 719-725. http://dx.doi.org/10.1007/s00705-014-2328-4[CrossRef]
  17. Mo, Q.H., Wang, H.B., Tan, H., Wu, B.M., Feng, Z.L., Wang, Q., Lin, J.C., Yang, Z., 2015b. Comparative detection of rotavirus RNA by conventional RT-PCR, TaqMan RT-PCR and real-time nucleic acid sequence-based amplification. Journal of virological methods 213, 1-4. http://dx.doi.org/10.1016/j.jviromet.2014.11.006[CrossRef]
  18. Mollasalehi, H., Yazdanparast, R., 2013. Development and evaluation of a novel nucleic acid sequence-based amplification method using one specific primer and one degenerate primer for simultaneous detection of Salmonella Enteritidis and Salmonella Typhimurium. Analytica chimica acta 770, 169-174. http://dx.doi.org/10.1016/j.aca.2013.01.053[CrossRef]
  19. Parashar, U.D., Gibson, C.J., Bresee, J.S., Glass, R.I., 2006. Rotavirus and severe childhood diarrhea. Emerging infectious diseases 12, 304-306. http://dx.doi.org/10.3201/eid1202.050006[CrossRef]
  20. Patterson, S.S., Smith, M.W., Casper, E.T., Huffman, D., Stark, L., Fries, D., Paul, J.H., 2006. A nucleic acid sequence-based amplification assay for real-time detection of norovirus genogroup II. Journal of applied microbiology 101, 956-963. http://dx.doi.org/10.1111/j.1365-2672.2006.02934.x[CrossRef]
  21. Rohayem, J., Berger, S., Juretzek, T., Herchenroder, O., Mogel, M., Poppe, M., Henker, J., Rethwilm, A., 2004. A simple and rapid single-step multiplex RT-PCR to detect Norovirus, Astrovirus and Adenovirus in clinical stool samples. Journal of virological methods 118, 49-59. http://dx.doi.org/10.1016/j.jviromet.2004.01.016[CrossRef]
  22. Roman, E., Negredo, A., Dalton, R.M., Wilhelmi, I., Sanchez-Fauquier, A., 2002. Molecular detection of human calicivirus among Spanish children with acute gastroenteritis. Journal of clinical microbiology 40, 3857-3859. http://dx.doi.org/10.1128/JCM.40.10.3857-3859.2002[CrossRef]
  23. Tai, J.H., Ewert, M.S., Belliot, G., Glass, R.I., Monroe, S.S., 2003. Development of a rapid method using nucleic acid sequence-based amplification for the detection of astrovirus. Journal of virological methods 110, 119-127. http://dx.doi.org/10.1016/S0166-0934(03)00108-3[CrossRef]
  24. Tran, A., Talmud, D., Lejeune, B., Jovenin, N., Renois, F., Payan, C., Leveque, N., Andreoletti, L., 2010. Prevalence of rotavirus, adenovirus, norovirus, and astrovirus infections and coinfections among hospitalized children in northern France. Journal of clinical microbiology 48, 1943-1946. http://dx.doi.org/10.1128/JCM.02181-09[CrossRef]
  25. van der Meide, W.F., Schoone, G.J., Faber, W.R., Zeegelaar, J.E., de Vries, H.J., Ozbel, Y., Lai, A.F.R.F., Coelho, L.I., Kassi, M., Schallig, H.D., 2005. Quantitative nucleic acid sequence-based assay as a new molecular tool for detection and quantification of Leishmania parasites in skin biopsy samples. Journal of clinical microbiology 43, 5560-5566. http://dx.doi.org/10.1128/JCM.43.11.5560-5566.2005[CrossRef]
  26. van Maarseveen, N.M., Wessels, E., de Brouwer, C.S., Vossen, A.C., Claas, E.C., 2010. Diagnosis of viral gastroenteritis by simultaneous detection of Adenovirus group F, Astrovirus, Rotavirus group A, Norovirus genogroups I and II, and Sapovirus in two internally controlled multiplex real-time PCR assays. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 49, 205-210.
  27. Wahnschaffe, U., Schulzke, J.D., Zeitz, M., Ullrich, R., 2007. Predictors of clinical response to gluten-free diet in patients diagnosed with diarrhea-predominant irritable bowel syndrome. Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association 5, 844-850; quiz 769.
  28. Waters, V., Ford-Jones, E.L., Petric, M., Fearon, M., Corey, P., Moineddein, R., 2000. Etiology of community-acquired pediatric viral diarrhea: a prospective longitudinal study in hospitals, emergency departments, pediatric practices and child care centers during the winter rotavirus outbreak, 1997 to 1998. The Pediatric Rotavirus Epidemiology Study for Immunization Study Group. The Pediatric infectious disease journal 19, 843-848. http://dx.doi.org/10.1097/00006454-200009000-00007[CrossRef]
  29. Zhao, Y., Park, S., Kreiswirth, B.N., Ginocchio, C.C., Veyret, R., Laayoun, A., Troesch, A., Perlin, D.S., 2009. Rapid real-time nucleic Acid sequence-based amplification-molecular beacon platform to detect fungal and bacterial bloodstream infections. Journal of clinical microbiology 47, 2067-2078. http://dx.doi.org/10.1128/JCM.02230-08[CrossRef]

Related articles

Services

Article usage statistics

Loading…

RT-NASBA: An Ideal Approach for the Detection of Diarrhoea-Related Viruses · ResearchersLinks