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Short Communication · Volume 2, Issue 10, Pages 592–598, 21 November 2014 · Open Access

Reduced Minimum Inhibitory Concentration of Antibiotics Associated with DT 104 Phage Type of <i>Salmonella</i> <i>Enterica</i> <i>Serovar</i> <i>Typhimurium</i>

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Journal:
Advances in Animal and Veterinary Sciences
Published:
21 November 2014
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From the article

  • Reduced Minimum Inhibitory Concentration of Antibiotics Associated with DT 104 Phage Type of Salmonella Enterica Serovar Typhimurium.
  • A study was undertaken to illuminate the MIC levels of five antibiotics among Indian isolates of S. typhimurium.
  • In this study, we used HiComb MIC test for determining the minimum inhibitory concentration of selected antibiotics (ampicillin, chloramphenicol, streptomycin, sulfisoxazole and tetracyclin) against which S. typhimurium isolates were found to be sensitive.
  • MICs of sulfisoxazole sensitive strains ranged between 3.0 μg/ml to >240 μg/ml. MIC level of 3 μg/ml was recorded in 6 isolates, 5 μg/ml in 1, greater than 240 μg/ml in 1 isolate.

Selected from the article's own text.

Abstract

Mudasir Ahmad1, Rajesh Kumar Agarwal2, Jinu Mary John3, Joy Lalmuanpuia Kataria1, Blessa Sailo4
1Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India; 2National Salmonella Centre (Vet), Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India; 3College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut; 4ICAR Research Complex for NEH region, Manipur Center, Lamphelpat, Imphal, India.

*Correspondence | Rajesh Kumar Agarwal, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., India; Email: grace_bly@yahoo.com

Abstract
There has been worldwide emergence of a multi-drug resistant S. typhimurium phage type DT104. It is reported to be resistant to five antibiotics viz., ampicillin, chloramphenicol, streptomycin, sulphonamide and tetracycline (ACSSuT) and a major health concern for human and animals alike. A study was undertaken to illuminate the MIC levels of five antibiotics among Indian isolates of S. typhimurium. Examination of 100 strains of S. typhimurium revealed that all the strains were sensitive to chloremphenicol and streptomycin and 95% of tested strains were sensitive to ampicilline. However, sulfisoxazole and tetracycline expressed resistance of 93% and 57%, respectively. The MIC values of all the antibiotics determined in this study were much below the break point except for one isolate, which showed- MICvalue of >240 μg/ml for sulfisoxazole. The study provides valuable information on the MIC value of S. typhimurium and it appears that this organism circulating in India have not yet acquired resistance against most of these antibiotics. Judicious use of these antibiotics in human and veterinary practice and regular monitoring is required to keep a check on the situation.

Keywords | S. typhimurium, DT 104 phage type, Indian, Antibiogram, Multi drug resistance

Acknowledgements

The authors are thankful to the Director, Indian Veterinary Research Institute, Izatnagar (U.P) for providing all the necessary facilities to carry out the above research work.

Conflict of Interest

There is no conflict of interest.

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Reduced Minimum Inhibitory Concentration of Antibiotics Associated with DT 104 Phage Type of <i>Salmonella</i> <i>Enterica</i> <i>Serovar</i> <i>Typhimurium</i> · ResearchersLinks